LOCUS       BBa_K331028              988 bp    DNA     linear       25-Nov-2024
DEFINITION  Lac Inducible N-terminal Arg Tagged ECFP (no terminator)
ACCESSION   BBa_K331028
VERSION     BBa_K331028.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     misc_feature    230..268
                     /label=Arginine Tag
     misc_feature    983..988
                     /label=double stop
     misc_feature    227..988
                     /label=BBa_K331001
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gccgccgccg
      241 ccgccgccgc cgccgccgca ctagaatggt gagcaagggc gaggagctgt tcaccggggt
      301 ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc cacaagttca gcgtgtccgg
      361 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      421 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt
      481 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      541 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      601 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      661 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta
      721 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
      781 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      841 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
      901 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      961 cggcatggac gagctgtaca agtaataa
//