LOCUS       BBa_K332022              908 bp    DNA     linear       17-May-2024
DEFINITION  Plux + RBS + mRFP + double terminator
ACCESSION   BBa_K332022
VERSION     BBa_K332022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..756
                     /label=mrfp1
     misc_feature    763..787
                     /label=Help:Barcodes
     CDS             82..787
                     /label=BBa_E1010
     terminator      796..908
                     /label=BBa_J61048
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt
      121 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt
      181 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg
      241 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt
      301 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg
      361 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg
      421 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt
      481 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa
      541 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac
      601 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac
      661 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag
      721 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt
      781 agatcgctac tagagccggc ttatcggtca gtttcacctg atttacgtaa aaacccgctt
      841 cggcgggttt ttgcttttgg aggggcagaa agatgaatga ctgtccacga cgctataccc
      901 aaaagaaa
//