LOCUS       BBa_K332032             1836 bp    DNA     linear       17-May-2024
DEFINITION  Constitutive promoter+37℃ induced RBS+tetR+double 
            terminator+Ptet+RBS+GFP+double terminator
ACCESSION   BBa_K332032
VERSION     BBa_K332032.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             90..95
                     /label=rbs
     misc_feature    66..71
                     /label=scar adaptation
     stem_loop       44..61
                     /label=stem_loop
     stem_loop       67..95
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    44..95
                     /label=BBa_K115002
     CDS             105..721
                     /label=tetR
     misc_feature    722..755
                     /label=SsrA
     misc_feature    762..786
                     /label=Help:Barcodes
     CDS             102..786
                     /label=BBa_C0040
     terminator      795..907
                     /label=BBa_J61048
     misc_feature    1..907
                     /label=BBa_K332031
     misc_feature    916..934
                     /label=TetR 1
     misc_feature    916..969
                     /label=BBa_R0040
     promoter        935..940
                     /label=-35
     misc_feature    941..959
                     /label=TetR 2
     promoter        958..963
                     /label=-10
     promoter        916..969
                     /label=BBa_R0040
     misc_feature    982..985
                     /label=conserved
     RBS             978..989
                     /label=BBa_B0034
     CDS             996..1715
                     /label=GFP protein
     CDS             996..1715
                     /label=BBa_E0040
     terminator      1724..1836
                     /label=BBa_J61048
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagggacaag caatgcttgc
       61 cttgaatagt aacttttgaa tagtgattca ggaggtacta gatgtccaga ttagataaaa
      121 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
      181 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa
      241 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact
      301 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta
      361 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag
      421 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac
      481 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg
      541 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc
      601 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct
      661 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt
      721 ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta
      781 gatcactact agagccggct tatcggtcag tttcacctga tttacgtaaa aacccgcttc
      841 ggcgggtttt tgcttttgga ggggcagaaa gatgaatgac tgtccacgac gctataccca
      901 aaagaaatac tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat
      961 actgagcact actagagaaa gaggagaaat actagatgcg taaaggagaa gaacttttca
     1021 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     1081 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     1141 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc
     1201 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
     1261 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
     1321 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
     1381 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
     1441 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
     1501 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     1561 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     1621 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     1681 ttacacatgg catggatgaa ctatacaaat aataatacta gagccggctt atcggtcagt
     1741 ttcacctgat ttacgtaaaa acccgcttcg gcgggttttt gcttttggag gggcagaaag
     1801 atgaatgact gtccacgacg ctatacccaa aagaaa
//