##gff-version 3
##sequence-region BBa_K332032 1 1836
BBa_K332032	.	promoter	916	969	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K332032	.	engineered_region	916	969	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K332032	.	non_covalent_binding_site	916	934	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K332032	.	non_covalent_binding_site	941	959	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K332032	.	promoter	935	940	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K332032	.	promoter	958	963	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K332032	.	CDS	996	1715	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K332032	.	CDS	996	1715	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K332032	.	engineered_region	1	907	.	+	0	ID=BBa_K332031;Name=BBa_K332031
BBa_K332032	.	CDS	102	786	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K332031
BBa_K332032	.	tag	722	755	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K332032	.	engineered_tag	762	786	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K332032	.	CDS	105	721	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K332032	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K332031
BBa_K332032	.	terminator	795	907	.	+	0	ID=BBa_J61048;Name=BBa_J61048;Parent=BBa_K332031
BBa_K332032	.	mature_transcript_region	44	95	.	+	0	ID=BBa_K115002;Name=BBa_K115002;Parent=BBa_K332031
BBa_K332032	.	stem_loop	67	95	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K332032	.	sequence_alteration	66	71	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
BBa_K332032	.	ribosome_entry_site	90	95	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K332032	.	stem_loop	44	61	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
BBa_K332032	.	ribosome_entry_site	978	989	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K332032	.	conserved_region	982	985	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K332032	.	terminator	1724	1836	.	+	0	ID=BBa_J61048;Name=BBa_J61048
>BBa_K332032
tttacagctagctcagtcctaggtattatgctagctactagagggacaagcaatgcttgccttgaatagtaacttttga
atagtgattcaggaggtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgag
gtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaa
aaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagg
ggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaa
gtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtt
tttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagca
tcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattattt
gatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtg
aaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactact
agagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaa
agatgaatgactgtccacgacgctatacccaaaagaaatactagagtccctatcagtgatagagattgacatccctatc
agtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagt
tgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgca
acatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactt
tcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccga
aggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggt
gatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaat
acaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagaca
caacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttta
ccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttg
agtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccggcttatcggtca
gtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacg
acgctatacccaaaagaaa