BBa_K332032
type: ComponentDefinition
persistent identity: BBa K332032
display id: BBa_K332032
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive promoter+37℃ induced RBS+tetR+double terminator+Ptet+RBS+GFP+double terminator
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: We want to utilize a temperature-regulated mechanism to control the expression period of a crystal protein. Therefore, we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???.
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: KUO, CHIA-CHUN
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_J61048
component: BBa_E0040
component: BBa_B0034
component: BBa_R0040
component: BBa_K332031
sequence annotation: BBa_K332031
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K332032_sequence/1

BBa_J61048
type: Component
persistent identity: component2273256
display id: component2273256
version: 1
top level: BBa_K332032
definition: BBa_J61048
access: public

BBa_E0040
type: Component
persistent identity: component2273255
display id: component2273255
version: 1
top level: BBa_K332032
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component2273253
display id: component2273253
version: 1
top level: BBa_K332032
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component2273247
display id: component2273247
version: 1
top level: BBa_K332032
definition: p(tetR)
access: public

BBa_K332031
type: Component
persistent identity: component2273246
display id: component2273246
version: 1
top level: BBa_K332032
definition: BBa_K332031
access: public

BBa_K332031
type: SequenceAnnotation
persistent identity: annotation2273246
display id: annotation2273246
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
component: BBa_K332031

https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
type: Range
persistent identity: range2273246
display id: range2273246
version: 1
top level: BBa_K332032
direction: direction/reverse
start: 1
end: 907
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2273247
display id: annotation2273247
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
type: Range
persistent identity: range2273247
display id: range2273247
version: 1
top level: BBa_K332032
direction: direction/forward
start: 916
end: 969
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273253
display id: annotation2273253
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
type: Range
persistent identity: range2273253
display id: range2273253
version: 1
top level: BBa_K332032
direction: direction/reverse
start: 978
end: 989
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2273255
display id: annotation2273255
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
type: Range
persistent identity: range2273255
display id: range2273255
version: 1
top level: BBa_K332032
direction: direction/reverse
start: 996
end: 1715
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2273256
display id: annotation2273256
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
type: Range
persistent identity: range2273256
display id: range2273256
version: 1
top level: BBa_K332032
direction: direction/reverse
start: 1724
end: 1836
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K332031
type: ComponentDefinition
persistent identity: BBa K332031
display id: BBa_K332031
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive promoter + 37℃ induced RBS + tetR + double terminator
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
mutable provenance: iGEM08_TUDelft
owned by: james
owned by: myers
mutable description: we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???. We speculate that when the ???Regulator??? and ???Production??? sections are combined, we can achieve a high bacteria growth rate and close to no target protein producing rate at T>37??C, and low bacteria growth rate and high target protein producing rate at T<37??C.
discontinued: false
dominant: true
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: To regulate following gene by temperature
star: false
creator: KUO, CHIA-CHUN
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0040
component: BBa_K115002
component: BBa_J23101
component: BBa_J61048
sequence annotation: BBa_J23101
sequence annotation: BBa_K115002
sequence annotation: BBa_C0040
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K332031_sequence/1

BBa_C0040
type: Component
persistent identity: component2250406
display id: component2250406
version: 1
top level: BBa_K332031
definition: tetR
access: public

BBa_K115002
type: Component
persistent identity: component2250402
display id: component2250402
version: 1
top level: BBa_K332031
definition: BBa_K115002
access: public

BBa_J23101
type: Component
persistent identity: component2250397
display id: component2250397
version: 1
top level: BBa_K332031
definition: BBa_J23101
access: public

BBa_J61048
type: Component
persistent identity: component2250407
display id: component2250407
version: 1
top level: BBa_K332031
definition: BBa_J61048
access: public

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2250397
display id: annotation2250397
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
type: Range
persistent identity: range2250397
display id: range2250397
version: 1
top level: BBa_K332031
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2250402
display id: annotation2250402
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
type: Range
persistent identity: range2250402
display id: range2250402
version: 1
top level: BBa_K332031
direction: direction/reverse
start: 44
end: 95
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2250406
display id: annotation2250406
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
type: Range
persistent identity: range2250406
display id: range2250406
version: 1
top level: BBa_K332031
direction: direction/reverse
start: 102
end: 786
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2250407
display id: annotation2250407
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
type: Range
persistent identity: range2250407
display id: range2250407
version: 1
top level: BBa_K332031
direction: direction/reverse
start: 795
end: 907
orientation: inline

BBa_J61048
type: ComponentDefinition
persistent identity: BBa J61048
display id: BBa_J61048
version: 1
was generated by: iGEM to SBOL conversion
description: [rnpB-T1] Terminator
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bob
star: true
creator: John Anderson
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_J61048_sequence/1

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_K115002
type: ComponentDefinition
persistent identity: BBa K115002
display id: BBa_K115002
version: 1
was generated by: iGEM to SBOL conversion
description: RNA thermometer (FourU)
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:27Z
mutable provenance: Salmonella Enterica Tyhpy (CP000886.1)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Thermo sensitive small RNA (ROSE structure) which can be used as a RNA regulator.
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3006
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Part of the sequence is altered because of the scar...
star: true
creator: Bastiaan van den Berg
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: stem_loop
sequence annotation: scar adaptation
sequence annotation: predicted stem-loop extending to one position before start codon
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K115002_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1966930
display id: annotation1966930
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966930/range1966930/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K115002/annotation1966930/range1966930/1
type: Range
persistent identity: range1966930
display id: range1966930
version: 1
top level: BBa_K115002
direction: direction/forward
start: 1
end: 18
orientation: inline

scar adaptation
type: SequenceAnnotation
persistent identity: annotation1966899
display id: annotation1966899
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966899/range1966899/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K115002/annotation1966899/range1966899/1
type: Range
persistent identity: range1966899
display id: range1966899
version: 1
top level: BBa_K115002
direction: direction/forward
start: 23
end: 28
orientation: inline

predicted stem-loop extending to one position before start codon
type: SequenceAnnotation
persistent identity: annotation1966931
display id: annotation1966931
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966931/range1966931/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K115002/annotation1966931/range1966931/1
type: Range
persistent identity: range1966931
display id: range1966931
version: 1
top level: BBa_K115002
direction: direction/forward
start: 24
end: 52
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1966897
display id: annotation1966897
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966897/range1966897/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K115002/annotation1966897/range1966897/1
type: Range
persistent identity: range1966897
display id: range1966897
version: 1
top level: BBa_K115002
direction: direction/forward
start: 47
end: 52
orientation: inline

https://synbiohub.org/public/igem/BBa_K332032_sequence/1
type: Sequence
persistent identity: BBa K332032 sequence
display id: BBa_K332032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K332031_sequence/1
type: Sequence
persistent identity: BBa K332031 sequence
display id: BBa_K332031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61048_sequence/1
type: Sequence
persistent identity: BBa J61048 sequence
display id: BBa_J61048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K115002_sequence/1
type: Sequence
persistent identity: BBa K115002 sequence
display id: BBa_K115002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggagg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z