LOCUS       BBa_K332033              864 bp    DNA     linear       03-May-2024
DEFINITION  37℃ induced RBS + tetR + double terminator
ACCESSION   BBa_K332033
VERSION     BBa_K332033.1  
FEATURES             Location/Qualifiers
     RBS             47..52
                     /label=rbs
     misc_feature    23..28
                     /label=scar adaptation
     stem_loop       1..18
                     /label=stem_loop
     stem_loop       24..52
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    1..52
                     /label=BBa_K115002
     CDS             62..678
                     /label=tetR
     misc_feature    679..712
                     /label=SsrA
     misc_feature    719..743
                     /label=Help:Barcodes
     CDS             59..743
                     /label=BBa_C0040
     terminator      752..864
                     /label=BBa_J61048
ORIGIN
        1 ggacaagcaa tgcttgcctt gaatagtaac ttttgaatag tgattcagga ggtactagat
       61 gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg
      121 aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt
      181 gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga
      241 taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa
      301 taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt
      361 aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg
      421 ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac
      481 tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc
      541 tactactgat agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg
      601 tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact
      661 taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac gctttagtag cttaataaca
      721 ctgatagtgc tagtgtagat cactactaga gccggcttat cggtcagttt cacctgattt
      781 acgtaaaaac ccgcttcggc gggtttttgc ttttggaggg gcagaaagat gaatgactgt
      841 ccacgacgct atacccaaaa gaaa
//