LOCUS       BBa_K332031              907 bp    DNA     linear       17-May-2024
DEFINITION  Constitutive promoter + 37℃ induced RBS + tetR + double 
            terminator
ACCESSION   BBa_K332031
VERSION     BBa_K332031.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             90..95
                     /label=rbs
     misc_feature    66..71
                     /label=scar adaptation
     stem_loop       44..61
                     /label=stem_loop
     stem_loop       67..95
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    44..95
                     /label=BBa_K115002
     CDS             105..721
                     /label=tetR
     misc_feature    722..755
                     /label=SsrA
     misc_feature    762..786
                     /label=Help:Barcodes
     CDS             102..786
                     /label=BBa_C0040
     terminator      795..907
                     /label=BBa_J61048
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagggacaag caatgcttgc
       61 cttgaatagt aacttttgaa tagtgattca ggaggtacta gatgtccaga ttagataaaa
      121 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
      181 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa
      241 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact
      301 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta
      361 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag
      421 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac
      481 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg
      541 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc
      601 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct
      661 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt
      721 ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta
      781 gatcactact agagccggct tatcggtcag tttcacctga tttacgtaaa aacccgcttc
      841 ggcgggtttt tgcttttgga ggggcagaaa gatgaatgac tgtccacgac gctataccca
      901 aaagaaa
//
LOCUS       BBa_K332034              907 bp    DNA     linear       17-May-2024
DEFINITION  Constitutive promoter + 37℃ induced RBS + tetR + double 
            terminator
ACCESSION   BBa_K332034
VERSION     BBa_K332034.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             90..95
                     /label=rbs
     misc_feature    66..71
                     /label=scar adaptation
     stem_loop       44..61
                     /label=stem_loop
     stem_loop       67..95
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    44..95
                     /label=BBa_K115002
     CDS             105..721
                     /label=tetR
     misc_feature    722..755
                     /label=SsrA
     misc_feature    762..786
                     /label=Help:Barcodes
     CDS             102..761
                     /label=BBa_C0040
     terminator      795..907
                     /label=BBa_J61048
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagggacaag caatgcttgc
       61 cttgaatagt aacttttgaa tagtgattca ggaggtacta gatgtccaga ttagataaaa
      121 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
      181 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa
      241 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact
      301 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta
      361 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag
      421 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac
      481 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg
      541 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc
      601 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct
      661 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt
      721 ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta
      781 gatcactact agagccggct tatcggtcag tttcacctga tttacgtaaa aacccgcttc
      841 ggcgggtttt tgcttttgga ggggcagaaa gatgaatgac tgtccacgac gctataccca
      901 aaagaaa
//