BBa_K115002
1
BBa_K115002
RNA thermometer (FourU)
2008-07-14T11:00:00Z
2015-05-08T01:09:27Z
Salmonella Enterica Tyhpy (CP000886.1)
Released HQ 2013
Thermo sensitive small RNA (ROSE structure) which can be used as a RNA regulator.
false
false
_223_
0
3006
9
In stock
true
Part of the sequence is altered because of the scar...
true
Bastiaan van den Berg
annotation1966931
1
predicted stem-loop extending to one position before start codon
range1966931
1
24
52
annotation1966930
1
stem_loop
range1966930
1
1
18
annotation1966899
1
scar adaptation
range1966899
1
23
28
annotation1966897
1
rbs
range1966897
1
47
52
BBa_J61048
1
BBa_J61048
[rnpB-T1] Terminator
2007-02-20T12:00:00Z
2015-08-31T02:03:00Z
bob
bob
false
false
_95_
0
483
95
In stock
false
bob
true
John Anderson
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_C0040
1
tetR
tetracycline repressor from transposon Tn10 (+LVA)
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
Released HQ 2013
Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.</P>
false
true
_1_
0
24
7
In stock
false
References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P> References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P>BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
annotation23329
1
tetR
range23329
1
4
620
annotation23330
1
SsrA
range23330
1
621
654
annotation2213989
1
Help:Barcodes
range2213989
1
661
685
BBa_K332034
1
BBa_K332034
Constitutive promoter + 37℃ induced RBS + tetR + double terminator
2010-10-23T11:00:00Z
2015-05-08T01:12:05Z
the 37℃ induced RBS is from iGEM08_TUDelft
To regulate following genes by temperature.
To maintain cells at 37?? C ↑ : the tetR protein be generated and repress Ptet's circuit.
To release cells at 37?? C ↓ : the tetR protein not be generated and do not repress Ptet's circuit.
The theoretical switching temperature of BBa_k115002, which was designed by Team TUDelft in 2008, is 37℃.
Thus, our thermosensing device A can be induced at this temperature. (The switching temperature can be modified by changing RBS according to different conditions.)
Once being swiched on, tetR will inhibit the corresponding promoter, pTet, which enables us to regulate the expression of cry gene and quorum sensing gene devices.
true
false
_452_
0
7059
9
Discontinued
false
To regulate following genes by temperature.
false
KUO, CHIA-CHUN
component2094017
1
BBa_J23101
component2094025
1
BBa_C0040
component2094027
1
BBa_J61048
component2094022
1
BBa_K115002
annotation2094027
1
BBa_J61048
range2094027
1
795
907
annotation2094017
1
BBa_J23101
range2094017
1
1
35
annotation2094022
1
BBa_K115002
range2094022
1
44
95
annotation2094025
1
BBa_C0040
range2094025
1
102
761
BBa_K332031
1
BBa_K332031
Constitutive promoter + 37℃ induced RBS + tetR + double terminator
2010-10-21T11:00:00Z
2015-05-08T01:12:05Z
iGEM08_TUDelft
we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit.
The following is a description of our prescribed strategy.
First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators.
We will call this part the ???Regulator???.
We speculate that when the ???Regulator??? and ???Production??? sections are combined, we can achieve a high bacteria growth rate and close to no target protein producing rate at T>37??C, and low bacteria growth rate and high target protein producing rate at T<37??C.
false
true
_452_
0
7059
9
It's complicated
true
To regulate following gene by temperature
false
KUO, CHIA-CHUN
component2250402
1
BBa_K115002
component2250397
1
BBa_J23101
component2250407
1
BBa_J61048
component2250406
1
BBa_C0040
annotation2250406
1
BBa_C0040
range2250406
1
102
786
annotation2250397
1
BBa_J23101
range2250397
1
1
35
annotation2250407
1
BBa_J61048
range2250407
1
795
907
annotation2250402
1
BBa_K115002
range2250402
1
44
95
BBa_K332034_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
BBa_J61048_sequence
1
ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
BBa_C0040_sequence
1
atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
BBa_K115002_sequence
1
ggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggagg
BBa_K332031_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z