BBa_K334000
1
BBa_K334000
Oxygen Dependent Degradation Domain
2010-07-20T11:00:00Z
2015-05-08T01:12:05Z
HIF-1a Gene
Part of the degradation domain taken from the HIF-1a protein in humans. Under normal oxygen conditions, prolyl hydroxylases target this domain for hydroxylation. The hydroxylated subunit makes the resultant protein a target for E3 ubiquin ligase when bound by the VHL gene product, leading to its proteasomal degradation. This can be used, when fused to a reporter protein, to indicate the activity of endogenous HIF-1a in real-time.
false
false
_464_
0
6891
9
Not in stock
false
Consulted literature assessing the activity of this domain under certain degrees of oxygen distress.
false
Sean Kearney
BBa_K334000_sequence
1
atgaacccgtttagcacccaggataccgatctggatctggaaatgctggcgccgtatattccgatggatgatgattttcagctgcgtagctttgatcagctgagcccgctggaaagcagcagcgcgagcccggaaagcgcgagcccgcagagcaccgtgaccgtgtttcagtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z