LOCUS       BBa_K336007             2107 bp    DNA     linear       22-Dec-2024
DEFINITION  p(tetR)+cinr+p(cinR)+mRFP1
ACCESSION   BBa_K336007
VERSION     BBa_K336007.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=cinR
     misc_feature    804..836
                     /label=LVA
     misc_feature    843..867
                     /label=Help:Barcodes
     CDS             81..867
                     /label=BBa_C0077
     stem_loop       887..930
                     /label=stem_loop
     misc_feature    876..955
                     /label=BBa_B0010
     terminator      876..955
                     /label=BBa_B0010
     stem_loop       971..990
                     /label=T7 TE
     misc_feature    997..997
                     /label=stop
     polyA_site      991..1004
                     /label=polya
     misc_feature    964..1004
                     /label=BBa_B0012
     terminator      964..1004
                     /label=BBa_B0012
     misc_feature    1013..1232
                     /label=unidentified/uncharacterized CinR dependent
     stem_loop       1047..1086
                     /label=stem_loop
     stem_loop       1103..1148
                     /label=stem_loop
     promoter        1013..1237
                     /label=BBa_R0078
     RBS             1246..1258
                     /label=RBS-3\Weak
     RBS             1252..1255
                     /label=RBS
     misc_feature    1246..1258
                     /label=BBa_B0032
     RBS             1246..1258
                     /label=BBa_B0032
     CDS             1265..1939
                     /label=mrfp1
     misc_feature    1946..1970
                     /label=Help:Barcodes
     CDS             1265..1970
                     /label=BBa_E1010
     stem_loop       1990..2033
                     /label=stem_loop
     misc_feature    1979..2058
                     /label=BBa_B0010
     terminator      1979..2058
                     /label=BBa_B0010
     stem_loop       2074..2093
                     /label=T7 TE
     misc_feature    2100..2100
                     /label=stop
     polyA_site      2094..2107
                     /label=polya
     misc_feature    2067..2107
                     /label=BBa_B0012
     terminator      2067..2107
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgattgaga atacctatag cgaaaagttc gagtccgcgt
      121 tcgaacagat caaggcggcg gccaacgtgg atgccgccat ccgtattctc caggcggaat
      181 ataacctcga tttcgtcacc taccatctcg cccagacgat cgcgagcaag atcgattcgc
      241 ccttcgtgcg caccacctat ccggatgcct gggtttcccg ctacctcctc aacagctatg
      301 tgaaggtcga tccgatcgtc aagcagggct tcgaacgcca gctgcccttc gactggagcg
      361 aggtcgaacc gacgccggag gcctatgcca tgctggtcga cgcccagaaa cacggcatcg
      421 gtggcaatgg ctactccatc cccgtcgccg acaaggcgca gcgccgcgcc ctgctgtcgc
      481 tgaatgcccg tataccggcc gacgaatgga ccgagctcgt gcgccgctgc cgcaacgagt
      541 ggatcgagat cgcccatctg atccaccgca aggccgtcta tgagctgcat ggcgaaaacg
      601 atccggtgcc ggcattgtcg ccgcgcgaga tcgagtgtct gcactggacc gccctcggca
      661 aggattacaa ggatatttcg gtcatcctgg gcatatcaga gcataccaca cgcgattacc
      721 tgaagaccgc ccgcttcaag ctcggctgcg ccacgatctc ggccgccgcg tcgcgggctg
      781 ttcaattgcg catcatcaat cccgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      841 aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc
      901 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      961 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agccctttgt
     1021 gcgtccaaac ggacgcacgg cgctctaaag cgggtcgcga tctttcagat tcgctcctcg
     1081 cgctttcagt ctttgttttg gcgcatgtcg ttatcgcaaa accgctgcac acttttgcgc
     1141 gacatgctct gatccccctc atctgggggg gcctatctga gggaatttcc gatccggctc
     1201 gcctgaacca ttctgctttc cacgaacttg aaaacgctac tagagtcaca caggaaagta
     1261 ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga
     1321 aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga
     1381 aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga
     1441 catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat
     1501 cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt
     1561 cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat
     1621 ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa
     1681 aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg
     1741 tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac
     1801 cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact
     1861 ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg
     1921 tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagcc
     1981 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2041 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2101 gtttata
//