##gff-version 3
##sequence-region BBa_K336011 1 1975
BBa_K336011	.	terminator	1847	1926	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K336011	.	stem_loop	1858	1901	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K336011	.	engineered_region	1847	1926	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K336011	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K336011	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K336011	.	CDS	1133	1838	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K336011	.	engineered_tag	1814	1838	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K336011	.	CDS	1133	1807	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K336011	.	terminator	900	940	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K336011	.	polyA_site	927	940	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K336011	.	stem_loop	907	926	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K336011	.	stop_codon	933	933	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K336011	.	engineered_region	900	940	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K336011	.	ribosome_entry_site	1114	1126	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K336011	.	engineered_region	1114	1126	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K336011	.	ribosome_entry_site	1120	1123	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K336011	.	ribosome_entry_site	1114	1126	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K336011	.	CDS	81	803	.	+	0	ID=BBa_C0179;Name=BBa_C0179
BBa_K336011	.	terminator	812	891	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K336011	.	stem_loop	823	866	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K336011	.	engineered_region	812	891	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K336011	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K336011	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K336011	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K336011	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K336011	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K336011	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K336011	.	terminator	1935	1975	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K336011	.	polyA_site	1962	1975	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K336011	.	stem_loop	1942	1961	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K336011	.	stop_codon	1968	1968	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K336011	.	engineered_region	1935	1975	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K336011	.	promoter	949	1105	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_K336011	.	non_covalent_binding_site	994	1011	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K336011	.	promoter	1065	1070	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K336011	.	promoter	1088	1093	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K336011	.	non_covalent_binding_site	1054	1073	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
>BBa_K336011
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatg
gcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcg
tcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactg
tacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcc
tcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcg
tggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgc
actgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtg
ttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttcc
atatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttat
tactctctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagct
actagagtcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtat
ggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgct
aaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaag
cttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttat
gaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaa
ctgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgta
tgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctga
agttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacc
tcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgct
gatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata