LOCUS       BBa_K336017             1758 bp    DNA     linear       23-May-2024
DEFINITION  Plux α-amylase+ccdB
ACCESSION   BBa_K336017
VERSION     BBa_K336017.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..1089
                     /label=BBa_K336012
     CDS             81..1089
                     /label=BBa_K336012
     misc_feature    63..1089
                     /label=BBa_K336013
     stem_loop       1109..1152
                     /label=stem_loop
     misc_feature    1098..1177
                     /label=BBa_B0010
     terminator      1098..1177
                     /label=BBa_B0010
     stem_loop       1193..1212
                     /label=T7 TE
     misc_feature    1219..1219
                     /label=stop
     polyA_site      1213..1226
                     /label=polya
     misc_feature    1186..1226
                     /label=BBa_B0012
     terminator      1186..1226
                     /label=BBa_B0012
     misc_feature    1..1226
                     /label=BBa_K336015
     misc_feature    1254..1259
                     /label=-35 Region
     misc_feature    1276..1281
                     /label=-10 Region
     misc_feature    1235..1254
                     /label=LuxR 
     promoter        1235..1289
                     /label=BBa_K091156
     misc_feature    1302..1305
                     /label=conserved
     RBS             1298..1309
                     /label=BBa_B0034
     misc_feature    1316..1318
                     /label=start
     misc_feature    1619..1621
                     /label=stop
     CDS             1316..1618
                     /label=cds
     CDS             1316..1621
                     /label=BBa_K145151
     stem_loop       1641..1684
                     /label=stem_loop
     misc_feature    1630..1709
                     /label=BBa_B0010
     terminator      1630..1709
                     /label=BBa_B0010
     stem_loop       1725..1744
                     /label=T7 TE
     misc_feature    1751..1751
                     /label=stop
     polyA_site      1745..1758
                     /label=polya
     misc_feature    1718..1758
                     /label=BBa_B0012
     terminator      1718..1758
                     /label=BBa_B0012
     misc_feature    1235..1758
                     /label=BBa_K336016
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgttcatgg tgttttgatc attttaaatt tttatatggc
      121 gggtggtggg caactcgctt gcgcgggcaa ctcgcttacc gattacgtta gggctgatat
      181 ttacgtaaaa atcgtcaagg gatgcaagac caaagtagta aaaccccgga gtcaacagca
      241 tccaagccca agtccttcac ggagaaaccc cagcgtccac atcacgagcg aaggaccacc
      301 tctaggcatc ggacgcacca tccaattaga agcagcaaag cgaaacagcc caagaaaaag
      361 gtcggcccgt cggccttttc tgcaacgctg atcacgggca gcgatccaac caacaccctc
      421 cagagtgact aggggcggaa atttaaaggg attaatttcc actcaaccac aaatcacagt
      481 cgtccccggt attgtcctgc agaatgcaat ttaaactctt ctgcgaatcg cttggattcc
      541 ccgcccctgg ccgtagagct taaagtatgt cccttgtcga tgcgatgtat cacaacatat
      601 aaatactagc aagggatgcc atgcttggag gatagcaacc gacaacatca catcaagctc
      661 tcccttctct gaacaataaa ccccacagaa ggcatttatg atggtcgcgt ggtggtctct
      721 atttctgtac ggccttcagg tcgcggcacc tgctttggct gcaacgcctg cggactggcg
      781 atcgcaatcc atttatttcc ttctcacgga tcgatttgca aggacggatg ggtcgacgac
      841 tgcgacttgt aatactgcgg atcaggtgtg ttgttaccta ctagctttca gaaagaggaa
      901 tgtaaactga cttgatatag aaatactgtg gtggaacatg gcagggcatc atcgacaagg
      961 taaattgccc ctttatcaaa aaaaaagaag gaaaagcaga agaaaaataa aataaaaaga
     1021 actctagtcc taaccatcac atagttggac tatatccagg gaatgggctt cacagccatc
     1081 tggtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1141 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1201 ttcgggtggg cctttctgcg tttatatact agagacctgt aggatcgtac aggtttaatc
     1261 aagaaaatgg tttgttatag tcgaataaat actagagaaa gaggagaaat actagatgca
     1321 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     1381 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     1441 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     1501 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     1561 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     1621 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1681 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1741 ggcctttctg cgtttata
//