BBa_K336017
type: ComponentDefinition
persistent identity: BBa K336017
display id: BBa_K336017
version: 1
was generated by: iGEM to SBOL conversion
description: Plux α-amylase+ccdB
created: 2010-10-26T11:00:00Z
modified: 2015-07-09T12:59:49Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: Lux protein activates this promoter, and genarates α‐amylase and ccdB. CcdB leads the cell death.
discontinued: true
dominant: false
experience: experience/None
group u list: _458_
m user id: 4206
owner id: 5997
owning group id: 9
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable notes: none
star: false
creator: Yuki Shimahara
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K336016
component: BBa_K336015
sequence annotation: BBa_K336015
sequence annotation: BBa_K336016
sequence: https://synbiohub.org/public/igem/BBa_K336017_sequence/1

BBa_K336016
type: Component
persistent identity: component2107390
display id: component2107390
version: 1
top level: BBa_K336017
definition: BBa_K336016
access: public

BBa_K336015
type: Component
persistent identity: component2107373
display id: component2107373
version: 1
top level: BBa_K336017
definition: BBa_K336015
access: public

BBa_K336015
type: SequenceAnnotation
persistent identity: annotation2107373
display id: annotation2107373
version: 1
top level: BBa_K336017
location: https://synbiohub.org/public/igem/BBa_K336017/annotation2107373/range2107373/1
component: BBa_K336015

https://synbiohub.org/public/igem/BBa_K336017/annotation2107373/range2107373/1
type: Range
persistent identity: range2107373
display id: range2107373
version: 1
top level: BBa_K336017
direction: direction/reverse
start: 1
end: 1226
orientation: inline

BBa_K336016
type: SequenceAnnotation
persistent identity: annotation2107390
display id: annotation2107390
version: 1
top level: BBa_K336017
location: https://synbiohub.org/public/igem/BBa_K336017/annotation2107390/range2107390/1
component: BBa_K336016

https://synbiohub.org/public/igem/BBa_K336017/annotation2107390/range2107390/1
type: Range
persistent identity: range2107390
display id: range2107390
version: 1
top level: BBa_K336017
direction: direction/reverse
start: 1235
end: 1758
orientation: inline

BBa_K336015
type: ComponentDefinition
persistent identity: BBa K336015
display id: BBa_K336015
version: 1
was generated by: iGEM to SBOL conversion
description: p(tetR)+RBS+α-amylase+stop
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:05Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: p(tetR)+RBS+α-amylase
discontinued: false
dominant: false
experience: experience/None
group u list: _458_
m user id: 0
owner id: 5997
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Yuki Shimahara
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
component: BBa_K336013
sequence annotation: BBa_R0040
sequence annotation: BBa_K336013
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K336015_sequence/1

BBa_R0040
type: Component
persistent identity: component2107343
display id: component2107343
version: 1
top level: BBa_K336015
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component2107354
display id: component2107354
version: 1
top level: BBa_K336015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2107352
display id: component2107352
version: 1
top level: BBa_K336015
definition: BBa_B0010
access: public

BBa_K336013
type: Component
persistent identity: component2107351
display id: component2107351
version: 1
top level: BBa_K336015
definition: BBa_K336013
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2107343
display id: annotation2107343
version: 1
top level: BBa_K336015
location: https://synbiohub.org/public/igem/BBa_K336015/annotation2107343/range2107343/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K336015/annotation2107343/range2107343/1
type: Range
persistent identity: range2107343
display id: range2107343
version: 1
top level: BBa_K336015
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_K336013
type: SequenceAnnotation
persistent identity: annotation2107351
display id: annotation2107351
version: 1
top level: BBa_K336015
location: https://synbiohub.org/public/igem/BBa_K336015/annotation2107351/range2107351/1
component: BBa_K336013

https://synbiohub.org/public/igem/BBa_K336015/annotation2107351/range2107351/1
type: Range
persistent identity: range2107351
display id: range2107351
version: 1
top level: BBa_K336015
direction: direction/reverse
start: 63
end: 1089
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2107352
display id: annotation2107352
version: 1
top level: BBa_K336015
location: https://synbiohub.org/public/igem/BBa_K336015/annotation2107352/range2107352/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K336015/annotation2107352/range2107352/1
type: Range
persistent identity: range2107352
display id: range2107352
version: 1
top level: BBa_K336015
direction: direction/forward
start: 1098
end: 1177
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2107354
display id: annotation2107354
version: 1
top level: BBa_K336015
location: https://synbiohub.org/public/igem/BBa_K336015/annotation2107354/range2107354/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K336015/annotation2107354/range2107354/1
type: Range
persistent identity: range2107354
display id: range2107354
version: 1
top level: BBa_K336015
direction: direction/forward
start: 1186
end: 1226
orientation: inline

BBa_K336016
type: ComponentDefinition
persistent identity: BBa K336016
display id: BBa_K336016
version: 1
was generated by: iGEM to SBOL conversion
description: Plux+RBS+ccdB+stop
created: 2010-10-26T11:00:00Z
modified: 2015-07-09T01:00:37Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: Plux+RBS+ccdB+stop
discontinued: true
dominant: false
experience: experience/None
group u list: _458_
m user id: 4206
owner id: 5997
owning group id: 9
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable notes: none
star: false
creator: Yuki Shimahara
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_K145151
component: BBa_B0034
component: BBa_K091156
sequence annotation: BBa_K091156
sequence annotation: BBa_B0034
sequence annotation: BBa_K145151
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K336016_sequence/1

BBa_B0012
type: Component
persistent identity: component2104615
display id: component2104615
version: 1
top level: BBa_K336016
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2104613
display id: component2104613
version: 1
top level: BBa_K336016
definition: BBa_B0010
access: public

BBa_K145151
type: Component
persistent identity: component2104612
display id: component2104612
version: 1
top level: BBa_K336016
definition: ccdB
access: public

BBa_B0034
type: Component
persistent identity: component2104608
display id: component2104608
version: 1
top level: BBa_K336016
definition: BBa_B0034
access: public

BBa_K091156
type: Component
persistent identity: component2104606
display id: component2104606
version: 1
top level: BBa_K336016
definition: BBa_K091156
access: public

BBa_K091156
type: SequenceAnnotation
persistent identity: annotation2104606
display id: annotation2104606
version: 1
top level: BBa_K336016
location: https://synbiohub.org/public/igem/BBa_K336016/annotation2104606/range2104606/1
component: BBa_K091156

https://synbiohub.org/public/igem/BBa_K336016/annotation2104606/range2104606/1
type: Range
persistent identity: range2104606
display id: range2104606
version: 1
top level: BBa_K336016
direction: direction/reverse
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2104608
display id: annotation2104608
version: 1
top level: BBa_K336016
location: https://synbiohub.org/public/igem/BBa_K336016/annotation2104608/range2104608/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K336016/annotation2104608/range2104608/1
type: Range
persistent identity: range2104608
display id: range2104608
version: 1
top level: BBa_K336016
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_K145151
type: SequenceAnnotation
persistent identity: annotation2104612
display id: annotation2104612
version: 1
top level: BBa_K336016
location: https://synbiohub.org/public/igem/BBa_K336016/annotation2104612/range2104612/1
component: BBa_K145151

https://synbiohub.org/public/igem/BBa_K336016/annotation2104612/range2104612/1
type: Range
persistent identity: range2104612
display id: range2104612
version: 1
top level: BBa_K336016
direction: direction/reverse
start: 82
end: 387
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2104613
display id: annotation2104613
version: 1
top level: BBa_K336016
location: https://synbiohub.org/public/igem/BBa_K336016/annotation2104613/range2104613/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K336016/annotation2104613/range2104613/1
type: Range
persistent identity: range2104613
display id: range2104613
version: 1
top level: BBa_K336016
direction: direction/forward
start: 396
end: 475
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2104615
display id: annotation2104615
version: 1
top level: BBa_K336016
location: https://synbiohub.org/public/igem/BBa_K336016/annotation2104615/range2104615/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K336016/annotation2104615/range2104615/1
type: Range
persistent identity: range2104615
display id: range2104615
version: 1
top level: BBa_K336016
direction: direction/forward
start: 484
end: 524
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa K145151
display id: BBa_K145151
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB coding region
created: 2008-08-06T11:00:00Z
modified: 2015-07-08T03:14:50Z
mutable provenance: P1010
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the ccdB (control of cell death) gene.
discontinued: true
dominant: false
experience: experience/None
group u list: _257_
m user id: 4206
owner id: 2970
owning group id: 9
sample status: In stock
status: status/Deleted
bookmark: true
mutable notes: just one stop codon in the end
star: true
creator: Jonas Demeulemeester
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: cds
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K145151_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1970251
display id: annotation1970251
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970251/range1970251/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K145151/annotation1970251/range1970251/1
type: Range
persistent identity: range1970251
display id: range1970251
version: 1
top level: ccdB
direction: direction/forward
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1970253
display id: annotation1970253
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970253/range1970253/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K145151/annotation1970253/range1970253/1
type: Range
persistent identity: range1970253
display id: range1970253
version: 1
top level: ccdB
direction: direction/forward
start: 1
end: 303
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1970252
display id: annotation1970252
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970252/range1970252/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K145151/annotation1970252/range1970252/1
type: Range
persistent identity: range1970252
display id: range1970252
version: 1
top level: ccdB
direction: direction/forward
start: 304
end: 306
orientation: inline

BBa_K091156
type: ComponentDefinition
persistent identity: BBa K091156
display id: BBa_K091156
version: 1
was generated by: iGEM to SBOL conversion
description: pLux
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: This part was adapted from BBa_R0062.
owned by: james
owned by: myers
mutable description: The pLux' promoter is a modified version of BBa_R0062 containing the -35 region TTTAAT in place of TTTACG. Transcriptional activation is expected to occur in the presence of LuxR and AI-1, just as with R0062. The modified -35 region was designed to serve as a control for the evaluation of BBa_K091156, the pLux+ Las- promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This promoter is identical to pLux+ Las- with the exception of the Las box's absence
star: false
creator: Erin Feeney
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: LuxR
sequence annotation: -35 Region
sequence annotation: -10 Region
sequence: https://synbiohub.org/public/igem/BBa_K091156_sequence/1

LuxR
type: SequenceAnnotation
persistent identity: annotation1968422
display id: annotation1968422
version: 1
top level: BBa_K091156
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968422/range1968422/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K091156/annotation1968422/range1968422/1
type: Range
persistent identity: range1968422
display id: range1968422
version: 1
top level: BBa_K091156
direction: direction/forward
start: 1
end: 20
orientation: inline

-35 Region
type: SequenceAnnotation
persistent identity: annotation1968420
display id: annotation1968420
version: 1
top level: BBa_K091156
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968420/range1968420/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K091156/annotation1968420/range1968420/1
type: Range
persistent identity: range1968420
display id: range1968420
version: 1
top level: BBa_K091156
direction: direction/forward
start: 20
end: 25
orientation: inline

-10 Region
type: SequenceAnnotation
persistent identity: annotation1968421
display id: annotation1968421
version: 1
top level: BBa_K091156
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968421/range1968421/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K091156/annotation1968421/range1968421/1
type: Range
persistent identity: range1968421
display id: range1968421
version: 1
top level: BBa_K091156
direction: direction/forward
start: 42
end: 47
orientation: inline

BBa_K336013
type: ComponentDefinition
persistent identity: BBa K336013
display id: BBa_K336013
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+α-amylase
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:05Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: RBS+α-amylase
discontinued: false
dominant: false
group u list: _458_
m user id: 0
owner id: 5997
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Yuki Shimahara
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K336012
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K336012
sequence: https://synbiohub.org/public/igem/BBa_K336013_sequence/1

BBa_K336012
type: Component
persistent identity: component2106748
display id: component2106748
version: 1
top level: BBa_K336013
definition: α-amy
access: public

BBa_B0034
type: Component
persistent identity: component2106747
display id: component2106747
version: 1
top level: BBa_K336013
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2106747
display id: annotation2106747
version: 1
top level: BBa_K336013
location: https://synbiohub.org/public/igem/BBa_K336013/annotation2106747/range2106747/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K336013/annotation2106747/range2106747/1
type: Range
persistent identity: range2106747
display id: range2106747
version: 1
top level: BBa_K336013
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K336012
type: SequenceAnnotation
persistent identity: annotation2106748
display id: annotation2106748
version: 1
top level: BBa_K336013
location: https://synbiohub.org/public/igem/BBa_K336013/annotation2106748/range2106748/1
component: BBa_K336012

https://synbiohub.org/public/igem/BBa_K336013/annotation2106748/range2106748/1
type: Range
persistent identity: range2106748
display id: range2106748
version: 1
top level: BBa_K336013
direction: direction/reverse
start: 19
end: 1027
orientation: inline

α-amy
type: ComponentDefinition
persistent identity: BBa K336012
display id: BBa_K336012
version: 1
was generated by: iGEM to SBOL conversion
description: α-amylase
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:05Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: α-amylase from Aspergillus oryzae
discontinued: false
dominant: false
experience: experience/None
group u list: _458_
m user id: 0
owner id: 5997
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Yuki Shimahara
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_K336012
sequence: https://synbiohub.org/public/igem/BBa_K336012_sequence/1

BBa_K336012
type: SequenceAnnotation
persistent identity: annotation2104228
display id: annotation2104228
version: 1
top level: α-amy
location: https://synbiohub.org/public/igem/BBa_K336012/annotation2104228/range2104228/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K336012/annotation2104228/range2104228/1
type: Range
persistent identity: range2104228
display id: range2104228
version: 1
top level: α-amy
direction: direction/reverse
start: 1
end: 1009
orientation: inline

https://synbiohub.org/public/igem/BBa_K336017_sequence/1
type: Sequence
persistent identity: BBa K336017 sequence
display id: BBa_K336017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgttcatggtgttttgatcattttaaatttttatatggcgggtggtgggcaactcgcttgcgcgggcaactcgcttaccgattacgttagggctgatatttacgtaaaaatcgtcaagggatgcaagaccaaagtagtaaaaccccggagtcaacagcatccaagcccaagtccttcacggagaaaccccagcgtccacatcacgagcgaaggaccacctctaggcatcggacgcaccatccaattagaagcagcaaagcgaaacagcccaagaaaaaggtcggcccgtcggccttttctgcaacgctgatcacgggcagcgatccaaccaacaccctccagagtgactaggggcggaaatttaaagggattaatttccactcaaccacaaatcacagtcgtccccggtattgtcctgcagaatgcaatttaaactcttctgcgaatcgcttggattccccgcccctggccgtagagcttaaagtatgtcccttgtcgatgcgatgtatcacaacatataaatactagcaagggatgccatgcttggaggatagcaaccgacaacatcacatcaagctctcccttctctgaacaataaaccccacagaaggcatttatgatggtcgcgtggtggtctctatttctgtacggccttcaggtcgcggcacctgctttggctgcaacgcctgcggactggcgatcgcaatccatttatttccttctcacggatcgatttgcaaggacggatgggtcgacgactgcgacttgtaatactgcggatcaggtgtgttgttacctactagctttcagaaagaggaatgtaaactgacttgatatagaaatactgtggtggaacatggcagggcatcatcgacaaggtaaattgcccctttatcaaaaaaaaagaaggaaaagcagaagaaaaataaaataaaaagaactctagtcctaaccatcacatagttggactatatccagggaatgggcttcacagccatctggtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttaatcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K336015_sequence/1
type: Sequence
persistent identity: BBa K336015 sequence
display id: BBa_K336015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgttcatggtgttttgatcattttaaatttttatatggcgggtggtgggcaactcgcttgcgcgggcaactcgcttaccgattacgttagggctgatatttacgtaaaaatcgtcaagggatgcaagaccaaagtagtaaaaccccggagtcaacagcatccaagcccaagtccttcacggagaaaccccagcgtccacatcacgagcgaaggaccacctctaggcatcggacgcaccatccaattagaagcagcaaagcgaaacagcccaagaaaaaggtcggcccgtcggccttttctgcaacgctgatcacgggcagcgatccaaccaacaccctccagagtgactaggggcggaaatttaaagggattaatttccactcaaccacaaatcacagtcgtccccggtattgtcctgcagaatgcaatttaaactcttctgcgaatcgcttggattccccgcccctggccgtagagcttaaagtatgtcccttgtcgatgcgatgtatcacaacatataaatactagcaagggatgccatgcttggaggatagcaaccgacaacatcacatcaagctctcccttctctgaacaataaaccccacagaaggcatttatgatggtcgcgtggtggtctctatttctgtacggccttcaggtcgcggcacctgctttggctgcaacgcctgcggactggcgatcgcaatccatttatttccttctcacggatcgatttgcaaggacggatgggtcgacgactgcgacttgtaatactgcggatcaggtgtgttgttacctactagctttcagaaagaggaatgtaaactgacttgatatagaaatactgtggtggaacatggcagggcatcatcgacaaggtaaattgcccctttatcaaaaaaaaagaaggaaaagcagaagaaaaataaaataaaaagaactctagtcctaaccatcacatagttggactatatccagggaatgggcttcacagccatctggtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K336016_sequence/1
type: Sequence
persistent identity: BBa K336016 sequence
display id: BBa_K336016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttaatcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145151_sequence/1
type: Sequence
persistent identity: BBa K145151 sequence
display id: BBa_K145151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091156_sequence/1
type: Sequence
persistent identity: BBa K091156 sequence
display id: BBa_K091156_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttaatcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K336013_sequence/1
type: Sequence
persistent identity: BBa K336013 sequence
display id: BBa_K336013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgttcatggtgttttgatcattttaaatttttatatggcgggtggtgggcaactcgcttgcgcgggcaactcgcttaccgattacgttagggctgatatttacgtaaaaatcgtcaagggatgcaagaccaaagtagtaaaaccccggagtcaacagcatccaagcccaagtccttcacggagaaaccccagcgtccacatcacgagcgaaggaccacctctaggcatcggacgcaccatccaattagaagcagcaaagcgaaacagcccaagaaaaaggtcggcccgtcggccttttctgcaacgctgatcacgggcagcgatccaaccaacaccctccagagtgactaggggcggaaatttaaagggattaatttccactcaaccacaaatcacagtcgtccccggtattgtcctgcagaatgcaatttaaactcttctgcgaatcgcttggattccccgcccctggccgtagagcttaaagtatgtcccttgtcgatgcgatgtatcacaacatataaatactagcaagggatgccatgcttggaggatagcaaccgacaacatcacatcaagctctcccttctctgaacaataaaccccacagaaggcatttatgatggtcgcgtggtggtctctatttctgtacggccttcaggtcgcggcacctgctttggctgcaacgcctgcggactggcgatcgcaatccatttatttccttctcacggatcgatttgcaaggacggatgggtcgacgactgcgacttgtaatactgcggatcaggtgtgttgttacctactagctttcagaaagaggaatgtaaactgacttgatatagaaatactgtggtggaacatggcagggcatcatcgacaaggtaaattgcccctttatcaaaaaaaaagaaggaaaagcagaagaaaaataaaataaaaagaactctagtcctaaccatcacatagttggactatatccagggaatgggcttcacagccatctggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K336012_sequence/1
type: Sequence
persistent identity: BBa K336012 sequence
display id: BBa_K336012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcatggtgttttgatcattttaaatttttatatggcgggtggtgggcaactcgcttgcgcgggcaactcgcttaccgattacgttagggctgatatttacgtaaaaatcgtcaagggatgcaagaccaaagtagtaaaaccccggagtcaacagcatccaagcccaagtccttcacggagaaaccccagcgtccacatcacgagcgaaggaccacctctaggcatcggacgcaccatccaattagaagcagcaaagcgaaacagcccaagaaaaaggtcggcccgtcggccttttctgcaacgctgatcacgggcagcgatccaaccaacaccctccagagtgactaggggcggaaatttaaagggattaatttccactcaaccacaaatcacagtcgtccccggtattgtcctgcagaatgcaatttaaactcttctgcgaatcgcttggattccccgcccctggccgtagagcttaaagtatgtcccttgtcgatgcgatgtatcacaacatataaatactagcaagggatgccatgcttggaggatagcaaccgacaacatcacatcaagctctcccttctctgaacaataaaccccacagaaggcatttatgatggtcgcgtggtggtctctatttctgtacggccttcaggtcgcggcacctgctttggctgcaacgcctgcggactggcgatcgcaatccatttatttccttctcacggatcgatttgcaaggacggatgggtcgacgactgcgacttgtaatactgcggatcaggtgtgttgttacctactagctttcagaaagaggaatgtaaactgacttgatatagaaatactgtggtggaacatggcagggcatcatcgacaaggtaaattgcccctttatcaaaaaaaaagaaggaaaagcagaagaaaaataaaataaaaagaactctagtcctaaccatcacatagttggactatatccagggaatgggcttcacagccatctggtaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z