LOCUS BBa_K336019 3632 bp DNA linear 23-Dec-2024 DEFINITION α-amylase　generating all in one model ACCESSION BBa_K336019 VERSION BBa_K336019.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..1089 /label=BBa_K336012 CDS 81..1089 /label=BBa_K336012 misc_feature 63..1089 /label=BBa_K336013 stem_loop 1109..1152 /label=stem_loop misc_feature 1098..1177 /label=BBa_B0010 terminator 1098..1177 /label=BBa_B0010 stem_loop 1193..1212 /label=T7 TE misc_feature 1219..1219 /label=stop polyA_site 1213..1226 /label=polya misc_feature 1186..1226 /label=BBa_B0012 terminator 1186..1226 /label=BBa_B0012 misc_feature 1..1226 /label=BBa_K336015 misc_feature 1235..1253 /label=TetR 1 misc_feature 1235..1288 /label=BBa_R0040 promoter 1254..1259 /label=-35 misc_feature 1260..1278 /label=TetR 2 promoter 1277..1282 /label=-10 promoter 1235..1288 /label=BBa_R0040 misc_feature 1301..1304 /label=conserved RBS 1297..1308 /label=BBa_B0034 misc_feature 1894..1925 /label=LVA CDS 1315..1893 /label=luxI misc_feature 1315..1932 /label=BBa_C0061 misc_feature 1933..1957 /label=Help:Barcodes CDS 1315..1932 /label=BBa_C0061 stem_loop 1977..2020 /label=stem_loop misc_feature 1966..2045 /label=BBa_B0010 terminator 1966..2045 /label=BBa_B0010 stem_loop 2061..2080 /label=T7 TE misc_feature 2087..2087 /label=stop polyA_site 2081..2094 /label=polya misc_feature 2054..2094 /label=BBa_B0012 terminator 2054..2094 /label=BBa_B0012 misc_feature 2103..2121 /label=TetR 1 misc_feature 2103..2156 /label=BBa_R0040 promoter 2122..2127 /label=-35 misc_feature 2128..2146 /label=TetR 2 promoter 2145..2150 /label=-10 promoter 2103..2156 /label=BBa_R0040 misc_feature 2169..2172 /label=conserved RBS 2165..2176 /label=BBa_B0034 misc_feature 2183..2184 /label=prefix misc_feature 2356..2356 /label=T misc_feature 2674..2674 /label=A CDS 2183..2932 /label=luxR misc_feature 2183..2938 /label=BBa_C0062 misc_feature 2939..2963 /label=Help:Barcodes CDS 2183..2938 /label=BBa_C0062 stem_loop 2983..3026 /label=stem_loop misc_feature 2972..3051 /label=BBa_B0010 terminator 2972..3051 /label=BBa_B0010 stem_loop 3067..3086 /label=T7 TE misc_feature 3093..3093 /label=stop polyA_site 3087..3100 /label=polya misc_feature 3060..3100 /label=BBa_B0012 terminator 3060..3100 /label=BBa_B0012 misc_feature 1235..3100 /label=BBa_K336018 misc_feature 3128..3133 /label=-35 Region misc_feature 3150..3155 /label=-10 Region misc_feature 3109..3128 /label=LuxR promoter 3109..3163 /label=BBa_K091156 misc_feature 3176..3179 /label=conserved RBS 3172..3183 /label=BBa_B0034 misc_feature 3190..3192 /label=start misc_feature 3493..3495 /label=stop CDS 3190..3492 /label=cds CDS 3190..3495 /label=BBa_K145151 stem_loop 3515..3558 /label=stem_loop misc_feature 3504..3583 /label=BBa_B0010 terminator 3504..3583 /label=BBa_B0010 stem_loop 3599..3618 /label=T7 TE misc_feature 3625..3625 /label=stop polyA_site 3619..3632 /label=polya misc_feature 3592..3632 /label=BBa_B0012 terminator 3592..3632 /label=BBa_B0012 misc_feature 3109..3632 /label=BBa_K336016 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgttcatgg tgttttgatc attttaaatt tttatatggc 121 gggtggtggg caactcgctt gcgcgggcaa ctcgcttacc gattacgtta gggctgatat 181 ttacgtaaaa atcgtcaagg gatgcaagac caaagtagta aaaccccgga gtcaacagca 241 tccaagccca agtccttcac ggagaaaccc cagcgtccac atcacgagcg aaggaccacc 301 tctaggcatc ggacgcacca tccaattaga agcagcaaag cgaaacagcc caagaaaaag 361 gtcggcccgt cggccttttc tgcaacgctg atcacgggca gcgatccaac caacaccctc 421 cagagtgact aggggcggaa atttaaaggg attaatttcc actcaaccac aaatcacagt 481 cgtccccggt attgtcctgc agaatgcaat ttaaactctt ctgcgaatcg cttggattcc 541 ccgcccctgg ccgtagagct taaagtatgt cccttgtcga tgcgatgtat cacaacatat 601 aaatactagc aagggatgcc atgcttggag gatagcaacc gacaacatca catcaagctc 661 tcccttctct gaacaataaa ccccacagaa ggcatttatg atggtcgcgt ggtggtctct 721 atttctgtac ggccttcagg tcgcggcacc tgctttggct gcaacgcctg cggactggcg 781 atcgcaatcc atttatttcc ttctcacgga tcgatttgca aggacggatg ggtcgacgac 841 tgcgacttgt aatactgcgg atcaggtgtg ttgttaccta ctagctttca gaaagaggaa 901 tgtaaactga cttgatatag aaatactgtg gtggaacatg gcagggcatc atcgacaagg 961 taaattgccc ctttatcaaa aaaaaagaag gaaaagcaga agaaaaataa aataaaaaga 1021 actctagtcc taaccatcac atagttggac tatatccagg gaatgggctt cacagccatc 1081 tggtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1141 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1201 ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag agattgacat 1261 ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata ctagatgact 1321 ataatgataa aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta 1381 agtcttcgtt atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac 1441 cttgaatcag atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact 1501 gaaaatgtaa gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt 1561 gtttttcctg aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta 1621 agtcgttttg ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca 1681 atgaaactat ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta 1741 acagtaacat caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt 1801 attggagaca aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct 1861 attaatgaac agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta 1921 gtagcttaat aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa 1981 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 2041 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 2101 agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact gagcactact 2161 agagaaagag gagaaatact agatgaaaaa cataaatgcc gacgacacat acagaataat 2221 taataaaatt aaagcttgta gaagcaataa tgatattaat caatgcttat ctgatatgac 2281 taaaatggta cattgtgaat attatttact cgcgatcatt tatcctcatt ctatggttaa 2341 atctgatatt tcaatcctag ataattaccc taaaaaatgg aggcaatatt atgatgacgc 2401 taatttaata aaatatgatc ctatagtaga ttattctaac tccaatcatt caccaattaa 2461 ttggaatata tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa ttaaagaagc 2521 gaaaacatca ggtcttatca ctgggtttag tttccctatt catacggcta acaatggctt 2581 cggaatgctt agttttgcac attcagaaaa agacaactat atagatagtt tatttttaca 2641 tgcgtgtatg aacataccat taattgttcc ttctctagtt gataattatc gaaaaataaa 2701 tatagcaaat aataaatcaa acaacgattt aaccaaaaga gaaaaagaat gtttagcgtg 2761 ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata ttaggttgca gtgagcgtac 2821 tgtcactttc catttaacca atgcgcaaat gaaactcaat acaacaaacc gctgccaaag 2881 tatttctaaa gcaattttaa caggagcaat tgattgccca tactttaaaa attaataaca 2941 ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag 3001 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 3061 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagac ctgtaggatc 3121 gtacaggttt aatcaagaaa atggtttgtt atagtcgaat aaatactaga gaaagaggag 3181 aaatactaga tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt 3241 gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc 3301 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg 3361 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg 3421 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg 3481 ttctggggaa tataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 3541 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 3601 ctcaccttcg ggtgggcctt tctgcgttta ta //