##gff-version 3
##sequence-region BBa_K336019 1 3632
BBa_K336019	.	engineered_region	3109	3632	.	+	0	ID=BBa_K336016;Name=BBa_K336016
BBa_K336019	.	ribosome_entry_site	3172	3183	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K336016
BBa_K336019	.	conserved_region	3176	3179	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K336019	.	CDS	3190	3495	.	+	0	ID=BBa_K145151;Name=BBa_K145151;Parent=BBa_K336016
BBa_K336019	.	stop_codon	3493	3495	.	+	0	ID=annotation1970252;Name=stop;Parent=BBa_K145151
BBa_K336019	.	start_codon	3190	3192	.	+	0	ID=annotation1970251;Name=start;Parent=BBa_K145151
BBa_K336019	.	CDS	3190	3492	.	+	0	ID=annotation1970253;Name=cds;Parent=BBa_K145151
BBa_K336019	.	terminator	3504	3583	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K336016
BBa_K336019	.	stem_loop	3515	3558	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K336019	.	engineered_region	3504	3583	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K336019	.	terminator	3592	3632	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K336016
BBa_K336019	.	stem_loop	3599	3618	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K336019	.	polyA_site	3619	3632	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K336019	.	stop_codon	3625	3625	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K336019	.	engineered_region	3592	3632	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K336019	.	promoter	3109	3163	.	+	0	ID=BBa_K091156;Name=BBa_K091156;Parent=BBa_K336016
BBa_K336019	.	non_covalent_binding_site	3109	3128	.	+	0	ID=annotation1968422;Name=LuxR ;Parent=BBa_K091156
BBa_K336019	.	sequence_feature	3150	3155	.	+	0	ID=annotation1968421;Name=-10 Region;Parent=BBa_K091156
BBa_K336019	.	sequence_feature	3128	3133	.	+	0	ID=annotation1968420;Name=-35 Region;Parent=BBa_K091156
BBa_K336019	.	engineered_region	1	1226	.	+	0	ID=BBa_K336015;Name=BBa_K336015
BBa_K336019	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K336015
BBa_K336019	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K336019	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K336019	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K336019	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K336019	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K336019	.	terminator	1098	1177	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K336015
BBa_K336019	.	stem_loop	1109	1152	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K336019	.	engineered_region	1098	1177	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K336019	.	terminator	1186	1226	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K336015
BBa_K336019	.	stem_loop	1193	1212	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K336019	.	polyA_site	1213	1226	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K336019	.	stop_codon	1219	1219	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K336019	.	engineered_region	1186	1226	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K336019	.	engineered_region	63	1089	.	+	0	ID=BBa_K336013;Name=BBa_K336013;Parent=BBa_K336015
BBa_K336019	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K336013
BBa_K336019	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K336019	.	CDS	81	1089	.	+	0	ID=BBa_K336012;Name=BBa_K336012;Parent=BBa_K336013
BBa_K336019	.	engineered_region	81	1089	.	+	0	ID=annotation2104228;Name=BBa_K336012;Parent=BBa_K336012
BBa_K336019	.	engineered_region	1235	3100	.	+	0	ID=BBa_K336018;Name=BBa_K336018
BBa_K336019	.	terminator	3060	3100	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K336018
BBa_K336019	.	stem_loop	3067	3086	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K336019	.	polyA_site	3087	3100	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K336019	.	stop_codon	3093	3093	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K336019	.	engineered_region	3060	3100	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K336019	.	ribosome_entry_site	1297	1308	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K336018
BBa_K336019	.	conserved_region	1301	1304	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K336019	.	terminator	1966	2045	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K336018
BBa_K336019	.	stem_loop	1977	2020	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K336019	.	engineered_region	1966	2045	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K336019	.	promoter	1235	1288	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K336018
BBa_K336019	.	promoter	1254	1259	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K336019	.	engineered_region	1235	1288	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K336019	.	non_covalent_binding_site	1235	1253	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K336019	.	non_covalent_binding_site	1260	1278	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K336019	.	promoter	1277	1282	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K336019	.	promoter	2103	2156	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K336018
BBa_K336019	.	promoter	2122	2127	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K336019	.	engineered_region	2103	2156	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K336019	.	non_covalent_binding_site	2103	2121	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K336019	.	non_covalent_binding_site	2128	2146	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K336019	.	promoter	2145	2150	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K336019	.	ribosome_entry_site	2165	2176	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K336018
BBa_K336019	.	conserved_region	2169	2172	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K336019	.	terminator	2972	3051	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K336018
BBa_K336019	.	stem_loop	2983	3026	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K336019	.	engineered_region	2972	3051	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K336019	.	CDS	1315	1932	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K336018
BBa_K336019	.	engineered_region	1315	1932	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K336019	.	CDS	1315	1893	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K336019	.	tag	1894	1925	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K336019	.	engineered_tag	1933	1957	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K336019	.	terminator	2054	2094	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K336018
BBa_K336019	.	stem_loop	2061	2080	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K336019	.	polyA_site	2081	2094	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K336019	.	stop_codon	2087	2087	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K336019	.	engineered_region	2054	2094	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K336019	.	CDS	2183	2938	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K336018
BBa_K336019	.	engineered_region	2183	2184	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K336019	.	engineered_tag	2939	2963	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K336019	.	point_mutation	2674	2674	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K336019	.	point_mutation	2356	2356	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K336019	.	engineered_region	2183	2938	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K336019	.	CDS	2183	2932	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
>BBa_K336019
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgttcatggtgttttgatcattttaaatttttatatggcgggtggtgggcaactcgcttgcgcgggcaactcgctta
ccgattacgttagggctgatatttacgtaaaaatcgtcaagggatgcaagaccaaagtagtaaaaccccggagtcaaca
gcatccaagcccaagtccttcacggagaaaccccagcgtccacatcacgagcgaaggaccacctctaggcatcggacgc
accatccaattagaagcagcaaagcgaaacagcccaagaaaaaggtcggcccgtcggccttttctgcaacgctgatcac
gggcagcgatccaaccaacaccctccagagtgactaggggcggaaatttaaagggattaatttccactcaaccacaaat
cacagtcgtccccggtattgtcctgcagaatgcaatttaaactcttctgcgaatcgcttggattccccgcccctggccg
tagagcttaaagtatgtcccttgtcgatgcgatgtatcacaacatataaatactagcaagggatgccatgcttggagga
tagcaaccgacaacatcacatcaagctctcccttctctgaacaataaaccccacagaaggcatttatgatggtcgcgtg
gtggtctctatttctgtacggccttcaggtcgcggcacctgctttggctgcaacgcctgcggactggcgatcgcaatcc
atttatttccttctcacggatcgatttgcaaggacggatgggtcgacgactgcgacttgtaatactgcggatcaggtgt
gttgttacctactagctttcagaaagaggaatgtaaactgacttgatatagaaatactgtggtggaacatggcagggca
tcatcgacaaggtaaattgcccctttatcaaaaaaaaagaaggaaaagcagaagaaaaataaaataaaaagaactctag
tcctaaccatcacatagttggactatatccagggaatgggcttcacagccatctggtaataatactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccct
atcagtgatagagatactgagcactactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattt
tttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggac
ttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactg
aaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttgg
tcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaat
aactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatg
taacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaat
tcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgct
gcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatcccta
tcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatac
agaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtac
attgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccc
taaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcat
tcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacat
caggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcaga
aaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataat
tatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcat
gcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgc
gcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatac
tttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttaatcaagaaaatggtttgttatagtcgaat
aaatactagagaaagaggagaaatactagatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtt
tgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtca
gataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggcca
gtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacct
gatgttctggggaatataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata