LOCUS       BBa_K336053             3018 bp    DNA     linear       17-May-2024
DEFINITION  Over expression system (T7 polymerase induced by lac promoter)
ACCESSION   BBa_K336053
VERSION     BBa_K336053.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     misc_feature    2876..2878
                     /label=stop
     misc_feature    2879..2881
                     /label=stop
     CDS             230..2875
                     /label=protein
     CDS             227..2881
                     /label=BBa_K145001
     stem_loop       2901..2944
                     /label=stem_loop
     misc_feature    2890..2969
                     /label=BBa_B0010
     terminator      2890..2969
                     /label=BBa_B0010
     stem_loop       2985..3004
                     /label=T7 TE
     misc_feature    3011..3011
                     /label=stop
     polyA_site      3005..3018
                     /label=polya
     misc_feature    2978..3018
                     /label=BBa_B0012
     terminator      2978..3018
                     /label=BBa_B0012
     terminator      2890..3018
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga acacgattaa
      241 catcgctaag aacgacttct ctgacatcga actggctgct atcccgttca acactctggc
      301 tgaccattac ggtgagcgtt tagctcgcga acagttggcc cttgagcatg agtcttacga
      361 gatgggtgaa gcacgcttcc gcaagatgtt tgagcgtcaa cttaaagctg gtgaggttgc
      421 ggataacgct gccgccaagc ctctcatcac taccctactc cctaagatga ttgcacgcat
      481 caacgactgg tttgaggaag tgaaagctaa gcgcggcaag cgcccgacag ccttccagtt
      541 cctgcaagaa atcaagccgg aagccgtagc gtacatcacc attaagacca ctctggcttg
      601 cctaaccagt gctgacaata caaccgttca ggctgtagca agcgcaatcg gtcgggccat
      661 tgaggacgag gctcgcttcg gtcgtatccg tgaccttgaa gctaagcact tcaagaaaaa
      721 cgttgaggaa caactcaaca agcgcgtagg gcacgtctac aagaaagcat ttatgcaagt
      781 tgtcgaggct gacatgctct ctaagggtct actcggtggc gaggcgtggt cttcgtggca
      841 taaggaagac tctattcatg taggagtacg ctgcatcgag atgctcattg agtcaaccgg
      901 aatggttagc ttacaccgcc aaaatgctgg cgtagtaggt caagactctg agactatcga
      961 actcgcacct gaatacgctg aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc
     1021 tccgatgttc caaccttgcg tagttcctcc taagccgtgg actggcatta ctggtggtgg
     1081 ctattgggct aacggtcgtc gtcctctggc gctggtgcgt actcacagta agaaagcact
     1141 gatgcgctac gaagacgttt acatgcctga ggtgtacaaa gcgattaaca ttgcgcaaaa
     1201 caccgcatgg aaaatcaaca agaaagtcct agcggtcgcc aacgtaatca ccaagtggaa
     1261 gcattgtccg gtcgaggaca tccctgcgat tgagcgtgaa gaactcccga tgaaaccgga
     1321 agacatcgac atgaatcctg aggctctcac cgcgtggaaa cgtgctgccg ctgctgtgta
     1381 ccgcaaggac aaggctcgca agtctcgccg tatcagcctt gagttcatgc ttgagcaagc
     1441 caataagttt gctaaccata aggccatctg gttcccttac aacatggact ggcgcggtcg
     1501 tgtttacgct gtgtcaatgt tcaacccgca aggtaacgat atgaccaaag gactgcttac
     1561 gctggcgaaa ggtaaaccaa tcggtaagga aggttactac tggctgaaaa tccacggtgc
     1621 aaactgtgcg ggtgtcgata aggttccgtt ccctgagcgc atcaagttca ttgaggaaaa
     1681 ccacgagaac atcatggctt gcgctaagtc tccactggag aacacttggt gggctgagca
     1741 agattctccg ttctgcttcc ttgcgttctg ctttgagtac gctggggtac agcaccacgg
     1801 cctgagctat aactgctccc ttccgctggc gtttgacggg tcttgctctg gcatccagca
     1861 cttctccgcg atgctccgag atgaggtagg tggtcgcgcg gttaacttgc ttcctagtga
     1921 aaccgttcag gacatctacg ggattgttgc taagaaagtc aacgagattc tacaagcaga
     1981 cgcaatcaat gggaccgata acgaagtagt taccgtgacc gatgagaaca ctggtgaaat
     2041 ctctgagaaa gtcaagctgg gcactaaggc actggctggt caatggctgg cttacggtgt
     2101 tactcgcagt gtgactaagc gttcagtcat gacgctggct tacgggtcca aagagttcgg
     2161 cttccgtcaa caagtgctgg aagataccat tcagccagct attgattccg gcaagggtct
     2221 gatgttcact cagccgaatc aggctgctgg atacatggct aagctgattt gggaatctgt
     2281 gagcgtgacg gtggtagctg cggttgaagc aatgaactgg cttaagtctg ctgctaagct
     2341 gctggctgct gaggtcaaag ataagaagac tggagagatt cttcgcaagc gttgcgctgt
     2401 gcattgggta actcctgatg gtttccctgt gtggcaggaa tacaagaagc ctattcagac
     2461 gcgcttgaac ctgatgttcc tcggtcagtt ccgcttacag cctaccatta acaccaacaa
     2521 agatagcgag attgatgcac acaaacagga gtctggtatc gctcctaact ttgtacacag
     2581 ccaagacggt agccaccttc gtaagactgt agtgtgggca cacgagaagt acggaatcga
     2641 atcttttgca ctgattcacg actccttcgg taccattccg gctgacgctg cgaacctgtt
     2701 caaagcagtg cgcgaaacta tggttgacac atatgagtct tgtgatgtac tggctgattt
     2761 ctacgaccag ttcgctgacc agttgcacga gtctcaattg gacaaaatgc cagcacttcc
     2821 ggctaaaggt aacttgaacc tccgtgacat cttagagtcg gacttcgcgt tcgcgtaata
     2881 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2941 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     3001 ggcctttctg cgtttata
//