LOCUS BBa_K336061 4533 bp DNA linear 14-May-2024 DEFINITION promoter+RBS+ompA signal+lacZ+double termnator+promoter+RBS+SBFP2+d ouble termnator ACCESSION BBa_K336061 VERSION BBa_K336061.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 227..289 /label=OmpA misc_feature 227..229 /label=Start Codon misc_feature 227..289 /label=BBa_K208003 misc_feature 1..289 /label=BBa_K208017 misc_feature 3319..3319 /label=C->T CDS 296..3370 /label=lacZ misc_feature 296..298 /label=START misc_feature 3368..3370 /label=STOP CDS 296..3370 /label=BBa_I732005 stem_loop 3386..3405 /label=T7 TE misc_feature 3412..3412 /label=stop polyA_site 3406..3419 /label=polya misc_feature 3379..3419 /label=BBa_B0012 terminator 3379..3419 /label=BBa_B0012 stem_loop 3440..3462 /label=stem_loop misc_feature 3428..3473 /label=BBa_B0011 terminator 3428..3473 /label=BBa_B0011 CDS 3482..3569 /label=end of LacI coding region (inactive) misc_feature 3482..3681 /label=BBa_R0010 misc_feature 3570..3607 /label=CAP binding site promoter 3618..3623 /label=-35 promoter 3642..3647 /label=-10 misc_feature 3647..3681 /label=LacI binding site misc_feature 3654..3654 /label=start promoter 3482..3681 /label=BBa_R0010 RBS 3690..3704 /label=RBS-1\Strong RBS 3697..3701 /label=RBS misc_feature 3690..3704 /label=BBa_B0030 RBS 3690..3704 /label=BBa_B0030 CDS 3711..4430 /label=BBa_K156010 stem_loop 4446..4465 /label=T7 TE misc_feature 4472..4472 /label=stop polyA_site 4466..4479 /label=polya misc_feature 4439..4479 /label=BBa_B0012 terminator 4439..4479 /label=BBa_B0012 stem_loop 4500..4522 /label=stem_loop misc_feature 4488..4533 /label=BBa_B0011 terminator 4488..4533 /label=BBa_B0011 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaaagacagc 241 tatcgcgatt gcagtggcac tggctggttt cgctaccgta gcgcaggcct actagatgac 301 catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt 361 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga 421 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc 481 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc 541 cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac 601 caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg 661 ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat 721 tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta 781 cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga 841 aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg aagatcagga 901 tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca 961 aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga 1021 ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg 1081 gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga 1141 gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg 1201 gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac 1261 gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct 1321 gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc 1381 tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca 1441 gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct 1501 gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt 1561 gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac 1621 gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga 1681 atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc 1741 ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc 1801 gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa 1861 aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc 1921 gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg 1981 tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa 2041 cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt 2101 ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa 2161 acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga 2221 atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa 2281 gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga 2341 actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt 2401 gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg 2461 tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac 2521 caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca 2581 gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg 2641 cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat 2701 tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc 2761 gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc 2821 gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag 2881 tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg 2941 gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg 3001 gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc 3061 attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac 3121 gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg 3181 ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg 3241 cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc 3301 gtcagtatcg gcggaatttc agctgagcgc cggtcgctac cattaccagt tggtctggtg 3361 tcaaaaataa tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 3421 actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta ttttactaga 3481 gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 3541 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 3601 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 3661 agcggataac aatttcacac atactagaga ttaaagagga gaaatactag atggttagca 3721 aaggtgaaga gctgtttact ggtgttgttc cgatcctggt tgaactggac ggtgatgtta 3781 acggccacaa gttctccgtg tctggcgaag gcgagggcga tgctacctac ggtaaactga 3841 ccctgaagtt catctgtacc actggtaaac tgccggtacc gtggccgacc ctggttacca 3901 ccctgagcca tggtgttcag tgttttgcac gttacccgga tcacatgaag cagcatgatt 3961 tcttcaagtc tgcgatgccg gagggttacg tccaggaacg cactatcttc ttcaaagacg 4021 acggcaacta taaaacccgt gccgaggtta agtttgaggg tgacaccctg gtcaaccgta 4081 ttgaactgaa aggtatcgac ttcaaagaag acggcaacat tctgggtcac aaactggagt 4141 acaacttcaa cagccataac gtgtacatca ccgccgataa acagaagaac ggcatcaaag 4201 ctaacttcaa gattcgtcac aacatcgaag atggtggtgt tcaactggct gatcactacc 4261 agcagaacac gccgatcggt gatggcccgg tactgctgcc tgacaaccat tatctgtcta 4321 cccagtctaa actgagcaaa gacccgaatg agaaacgtga tcacatggtg ctgctggagt 4381 tcgtgactgc ggcaggtatc acgctgggta tggacgaact gtataaatga tactagagtc 4441 acactggctc accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa 4501 agccagatta ttaatccggc ttttttatta ttt //