##gff-version 3
##sequence-region BBa_K336061 1 4533
BBa_K336061	.	engineered_region	1	289	.	+	0	ID=BBa_K208017;Name=BBa_K208017
BBa_K336061	.	tag	227	289	.	+	0	ID=BBa_K208003;Name=BBa_K208003;Parent=BBa_K208017
BBa_K336061	.	tag	227	289	.	+	0	ID=annotation2034604;Name=OmpA;Parent=BBa_K208003
BBa_K336061	.	start_codon	227	229	.	+	0	ID=annotation2034605;Name=Start Codon;Parent=BBa_K208003
BBa_K336061	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K208017
BBa_K336061	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K336061	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K336061	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K336061	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K336061	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K336061	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K336061	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K336061	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K208017
BBa_K336061	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K336061	.	terminator	4439	4479	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K336061	.	engineered_region	4439	4479	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K336061	.	stem_loop	4446	4465	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K336061	.	polyA_site	4466	4479	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K336061	.	stop_codon	4472	4472	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K336061	.	CDS	3711	4430	.	+	0	ID=BBa_K156010;Name=BBa_K156010
BBa_K336061	.	ribosome_entry_site	3690	3704	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K336061	.	ribosome_entry_site	3697	3701	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K336061	.	engineered_region	3690	3704	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K336061	.	ribosome_entry_site	3690	3704	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K336061	.	promoter	3482	3681	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K336061	.	non_covalent_binding_site	3570	3607	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K336061	.	promoter	3642	3647	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K336061	.	engineered_region	3482	3681	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K336061	.	CDS	3482	3569	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K336061	.	non_covalent_binding_site	3647	3681	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K336061	.	promoter	3618	3623	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K336061	.	start_codon	3654	3654	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K336061	.	terminator	3379	3419	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K336061	.	engineered_region	3379	3419	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K336061	.	stem_loop	3386	3405	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K336061	.	polyA_site	3406	3419	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K336061	.	stop_codon	3412	3412	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K336061	.	terminator	3428	3473	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_K336061	.	stem_loop	3440	3462	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K336061	.	engineered_region	3428	3473	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K336061	.	terminator	4488	4533	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_K336061	.	stem_loop	4500	4522	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K336061	.	engineered_region	4488	4533	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K336061	.	CDS	296	3370	.	+	0	ID=BBa_I732005;Name=BBa_I732005
BBa_K336061	.	CDS	296	3370	.	+	0	ID=annotation1936870;Name=lacZ;Parent=BBa_I732005
BBa_K336061	.	point_mutation	3319	3319	.	+	0	ID=annotation1936869;Name=C->T;Parent=BBa_I732005
BBa_K336061	.	stop_codon	3368	3370	.	+	0	ID=annotation1936872;Name=STOP;Parent=BBa_I732005
BBa_K336061	.	start_codon	296	298	.	+	0	ID=annotation1936871;Name=START;Parent=BBa_I732005
>BBa_K336061
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaaagac
agctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggcctactagatgaccatgattacggattca
ctggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctt
tcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcg
ctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtc
gtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgc
cgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggcca
gacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggac
agtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgct
ggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataa
accgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaa
gttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcg
gcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcga
aaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctg
attgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgt
tgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcagga
tatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctg
tgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccg
atgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgat
catctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatcct
tcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtgg
atgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgct
gatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtat
ccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggt
cggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcac
gccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtg
accagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtggcgctggatggtaagccgctggcaa
gcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgc
cgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcacatcagcgcctgg
cagcagtggcgtctggcggaaaacctcagtgtgacgctccccgccgcgtcccacgccatcccgcatctgaccaccagcg
aaatggatttttgcatcgagctgggtaataagcgttggcaatttaaccgccagtcaggctttctttcacagatgtggat
tggcgataaaaaacaactgctgacgccgctgcgcgatcagttcacccgtgcaccgctggataacgacattggcgtaagt
gaagcgacccgcattgaccctaacgcctgggtcgaacgctggaaggcggcgggccattaccaggccgaagcagcgttgt
tgcagtgcacggcagatacacttgctgatgcggtgctgattacgaccgctcacgcgtggcagcatcaggggaaaacctt
atttatcagccggaaaacctaccggattgatggtagtggtcaaatggcgattaccgttgatgttgaagtggcgagcgat
acaccgcatccggcgcggattggcctgaactgccagctggcgcaggtagcagagcgggtaaactggctcggattagggc
cgcaagaaaactatcccgaccgccttactgccgcctgttttgaccgctgggatctgccattgtcagacatgtatacccc
gtacgtcttcccgagcgaaaacggtctgcgctgcgggacgcgcgaattgaattatggcccacaccagtggcgcggcgac
ttccagttcaacatcagccgctacagtcaacagcaactgatggaaaccagccatcgccatctgctgcacgcggaagaag
gcacatggctgaatatcgacggtttccatatggggattggtggcgacgactcctggagcccgtcagtatcggcggaatt
tcagctgagcgccggtcgctaccattaccagttggtctggtgtcaaaaataatactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttac
tagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactgg
aaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttc
cggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatg
gttagcaaaggtgaagagctgtttactggtgttgttccgatcctggttgaactggacggtgatgttaacggccacaagt
tctccgtgtctggcgaaggcgagggcgatgctacctacggtaaactgaccctgaagttcatctgtaccactggtaaact
gccggtaccgtggccgaccctggttaccaccctgagccatggtgttcagtgttttgcacgttacccggatcacatgaag
cagcatgatttcttcaagtctgcgatgccggagggttacgtccaggaacgcactatcttcttcaaagacgacggcaact
ataaaacccgtgccgaggttaagtttgagggtgacaccctggtcaaccgtattgaactgaaaggtatcgacttcaaaga
agacggcaacattctgggtcacaaactggagtacaacttcaacagccataacgtgtacatcaccgccgataaacagaag
aacggcatcaaagctaacttcaagattcgtcacaacatcgaagatggtggtgttcaactggctgatcactaccagcaga
acacgccgatcggtgatggcccggtactgctgcctgacaaccattatctgtctacccagtctaaactgagcaaagaccc
gaatgagaaacgtgatcacatggtgctgctggagttcgtgactgcggcaggtatcacgctgggtatggacgaactgtat
aaatgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagc
cagattattaatccggcttttttattattt