BBa_K336061
type: ComponentDefinition
persistent identity: BBa K336061
display id: BBa_K336061
version: 1
was generated by: iGEM to SBOL conversion
description: promoter+RBS+ompA signal+lacZ+double termnator+promoter+RBS+SBFP2+double termnator
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:05Z
mutable provenance: Escherichia coli K12 genome
owned by: james
owned by: myers
mutable description: This device is mede up with BBa_K208017, BBa_I732005, BBa_B0012, BBa_B0011, BBa_R0010, BBa_B0030, BBa_K156010, BBa_B0012 and BBa_B0011. This device expresses CyaA (Adenylate cyclase) out of cytoplasm and mRFP1 in cytoplasm. This device have function of inhibiting transcription of the lac operon and show own expression level by coloring. The inhibiting system is catabolic repression.
discontinued: false
dominant: false
experience: experience/None
group u list: _458_
m user id: 0
owner id: 5965
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: immature device
star: false
creator: Ryuto Nitta
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0011
component: BBa_B0012
component: BBa_I732005
component: BBa_K208017
component: BBa_K156010
component: BBa_B0030
component: BBa_R0010
sequence annotation: BBa_K208017
sequence annotation: BBa_I732005
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_K156010
sequence: https://synbiohub.org/public/igem/BBa_K336061_sequence/1

BBa_B0011
type: Component
persistent identity: component2108952
display id: component2108952
version: 1
top level: BBa_K336061
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component2108948
display id: component2108948
version: 1
top level: BBa_K336061
definition: BBa_B0012
access: public

BBa_I732005
type: Component
persistent identity: component2108947
display id: component2108947
version: 1
top level: BBa_K336061
definition: LacZ
access: public

BBa_K208017
type: Component
persistent identity: component2108942
display id: component2108942
version: 1
top level: BBa_K336061
definition: BBa_K208017
access: public

BBa_B0011
type: Component
persistent identity: component2108969
display id: component2108969
version: 1
top level: BBa_K336061
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component2108965
display id: component2108965
version: 1
top level: BBa_K336061
definition: BBa_B0012
access: public

BBa_K156010
type: Component
persistent identity: component2108964
display id: component2108964
version: 1
top level: BBa_K336061
definition: SBFP2
access: public

BBa_B0030
type: Component
persistent identity: component2108962
display id: component2108962
version: 1
top level: BBa_K336061
definition: BBa_B0030
access: public

BBa_R0010
type: Component
persistent identity: component2108954
display id: component2108954
version: 1
top level: BBa_K336061
definition: LacI
access: public

BBa_K208017
type: SequenceAnnotation
persistent identity: annotation2108942
display id: annotation2108942
version: 1
top level: BBa_K336061
location: https://synbiohub.org/public/igem/BBa_K336061/annotation2108942/range2108942/1
component: BBa_K208017

https://synbiohub.org/public/igem/BBa_K336061/annotation2108942/range2108942/1
type: Range
persistent identity: range2108942
display id: range2108942
version: 1
top level: BBa_K336061
direction: direction/reverse
start: 1
end: 289
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation2108947
display id: annotation2108947
version: 1
top level: BBa_K336061
location: https://synbiohub.org/public/igem/BBa_K336061/annotation2108947/range2108947/1
component: BBa_I732005

https://synbiohub.org/public/igem/BBa_K336061/annotation2108947/range2108947/1
type: Range
persistent identity: range2108947
display id: range2108947
version: 1
top level: BBa_K336061
direction: direction/reverse
start: 296
end: 3370
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2108948
display id: annotation2108948
version: 1
top level: BBa_K336061
location: https://synbiohub.org/public/igem/BBa_K336061/annotation2108948/range2108948/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K336061/annotation2108948/range2108948/1
type: Range
persistent identity: range2108948
display id: range2108948
version: 1
top level: BBa_K336061
direction: direction/forward
start: 3379
end: 3419
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2108952
display id: annotation2108952
version: 1
top level: BBa_K336061
location: https://synbiohub.org/public/igem/BBa_K336061/annotation2108952/range2108952/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K336061/annotation2108952/range2108952/1
type: Range
persistent identity: range2108952
display id: range2108952
version: 1
top level: BBa_K336061
direction: direction/forward
start: 3428
end: 3473
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2108954
display id: annotation2108954
version: 1
top level: BBa_K336061
location: https://synbiohub.org/public/igem/BBa_K336061/annotation2108954/range2108954/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K336061/annotation2108954/range2108954/1
type: Range
persistent identity: range2108954
display id: range2108954
version: 1
top level: BBa_K336061
direction: direction/forward
start: 3482
end: 3681
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2108962
display id: annotation2108962
version: 1
top level: BBa_K336061
location: https://synbiohub.org/public/igem/BBa_K336061/annotation2108962/range2108962/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K336061/annotation2108962/range2108962/1
type: Range
persistent identity: range2108962
display id: range2108962
version: 1
top level: BBa_K336061
direction: direction/forward
start: 3690
end: 3704
orientation: inline

BBa_K156010
type: SequenceAnnotation
persistent identity: annotation2108964
display id: annotation2108964
version: 1
top level: BBa_K336061
location: https://synbiohub.org/public/igem/BBa_K336061/annotation2108964/range2108964/1
component: BBa_K156010

https://synbiohub.org/public/igem/BBa_K336061/annotation2108964/range2108964/1
type: Range
persistent identity: range2108964
display id: range2108964
version: 1
top level: BBa_K336061
direction: direction/reverse
start: 3711
end: 4430
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2108965
display id: annotation2108965
version: 1
top level: BBa_K336061
location: https://synbiohub.org/public/igem/BBa_K336061/annotation2108965/range2108965/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K336061/annotation2108965/range2108965/1
type: Range
persistent identity: range2108965
display id: range2108965
version: 1
top level: BBa_K336061
direction: direction/forward
start: 4439
end: 4479
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2108969
display id: annotation2108969
version: 1
top level: BBa_K336061
location: https://synbiohub.org/public/igem/BBa_K336061/annotation2108969/range2108969/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K336061/annotation2108969/range2108969/1
type: Range
persistent identity: range2108969
display id: range2108969
version: 1
top level: BBa_K336061
direction: direction/forward
start: 4488
end: 4533
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

LacZ
type: ComponentDefinition
persistent identity: BBa I732005
display id: BBa_I732005
version: 1
was generated by: iGEM to SBOL conversion
description: lacZ (encoding beta-galactosidase, full-length)
created: 2007-07-05T11:00:00Z
modified: 2015-08-31T04:07:56Z
mutable provenance: PCR amplified and standardized from BL21
owned by: james
owned by: myers
mutable description: LacZ encodes beta-galactosidase, an intracellular enzyme that cleaves the disaccharide lactose into glucose and galactose. Beta-galactosidase can also cleaves X-gal and ONPG into colorful products.
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: true
creator: Zhan Jian
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: lacZ
sequence annotation: C->T
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732005_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation1936871
display id: annotation1936871
version: 1
top level: LacZ
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936871/range1936871/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I732005/annotation1936871/range1936871/1
type: Range
persistent identity: range1936871
display id: range1936871
version: 1
top level: LacZ
direction: direction/forward
start: 1
end: 3
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1936870
display id: annotation1936870
version: 1
top level: LacZ
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936870/range1936870/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I732005/annotation1936870/range1936870/1
type: Range
persistent identity: range1936870
display id: range1936870
version: 1
top level: LacZ
direction: direction/forward
start: 1
end: 3075
orientation: inline

C->T
type: SequenceAnnotation
persistent identity: annotation1936869
display id: annotation1936869
version: 1
top level: LacZ
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936869/range1936869/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I732005/annotation1936869/range1936869/1
type: Range
persistent identity: range1936869
display id: range1936869
version: 1
top level: LacZ
direction: direction/forward
start: 3024
end: 3024
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1936872
display id: annotation1936872
version: 1
top level: LacZ
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936872/range1936872/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I732005/annotation1936872/range1936872/1
type: Range
persistent identity: range1936872
display id: range1936872
version: 1
top level: LacZ
direction: direction/forward
start: 3073
end: 3075
orientation: inline

SBFP2
type: ComponentDefinition
persistent identity: BBa K156010
display id: BBa_K156010
version: 1
was generated by: iGEM to SBOL conversion
description: SBFP2 (strongly enhanced blue fluorescent protein)
created: 2008-10-28T12:00:00Z
modified: 2016-01-26T09:51:52Z
mutable provenance: OFP was cloned from the tentacles of ''Cerianthus'' sp., a tube anemone. The original sequence was obtained from GenBank (VERSION AY296063.1, GI:31616578).
owned by: james
owned by: myers
mutable description: This part codes for orange fluorescent protein (OFP). Fluorescent proteins are useful tools for visualizing and quantifying protein expression in cells. OFP is yet another fluorescent protein, like GFP, that can be utilized as a reporter.
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 4206
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This part was codon-optimized for expression in ''E. coli''. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
star: false
creator: George McArthur IV
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K156010_sequence/1

BBa_K208017
type: ComponentDefinition
persistent identity: BBa K208017
display id: BBa_K208017
version: 1
was generated by: iGEM to SBOL conversion
description: Lac Promoter/RBS/OmpA Signal Peptide
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:24Z
mutable provenance: Composite
owned by: james
owned by: myers
mutable description: Lac
discontinued: false
dominant: false
experience: experience/None
group u list: _310_
m user id: 0
owner id: 3473
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Silver Fusion
star: false
creator: USU iGEM 2009
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_R0010
component: BBa_K208003
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K208003
sequence: https://synbiohub.org/public/igem/BBa_K208017_sequence/1

BBa_B0034
type: Component
persistent identity: component2055718
display id: component2055718
version: 1
top level: BBa_K208017
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2055710
display id: component2055710
version: 1
top level: BBa_K208017
definition: LacI
access: public

BBa_K208003
type: Component
persistent identity: component2055721
display id: component2055721
version: 1
top level: BBa_K208017
definition: BBa_K208003
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2055710
display id: annotation2055710
version: 1
top level: BBa_K208017
location: https://synbiohub.org/public/igem/BBa_K208017/annotation2055710/range2055710/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K208017/annotation2055710/range2055710/1
type: Range
persistent identity: range2055710
display id: range2055710
version: 1
top level: BBa_K208017
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2055718
display id: annotation2055718
version: 1
top level: BBa_K208017
location: https://synbiohub.org/public/igem/BBa_K208017/annotation2055718/range2055718/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K208017/annotation2055718/range2055718/1
type: Range
persistent identity: range2055718
display id: range2055718
version: 1
top level: BBa_K208017
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_K208003
type: SequenceAnnotation
persistent identity: annotation2055721
display id: annotation2055721
version: 1
top level: BBa_K208017
location: https://synbiohub.org/public/igem/BBa_K208017/annotation2055721/range2055721/1
component: BBa_K208003

https://synbiohub.org/public/igem/BBa_K208017/annotation2055721/range2055721/1
type: Range
persistent identity: range2055721
display id: range2055721
version: 1
top level: BBa_K208017
direction: direction/reverse
start: 227
end: 289
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K208003
type: ComponentDefinition
persistent identity: BBa K208003
display id: BBa_K208003
version: 1
was generated by: iGEM to SBOL conversion
description: OmpA Signal Peptide - Silver Fusion Compatible
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:24Z
mutable provenance: Anneal
owned by: james
owned by: myers
mutable description: OmpA
discontinued: false
dominant: false
experience: experience/None
group u list: _310_
m user id: 0
owner id: 3473
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Silver Fusion
star: false
creator: USU iGEM 2009
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: Start Codon
sequence annotation: OmpA
sequence: https://synbiohub.org/public/igem/BBa_K208003_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2034605
display id: annotation2034605
version: 1
top level: BBa_K208003
location: https://synbiohub.org/public/igem/BBa_K208003/annotation2034605/range2034605/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K208003/annotation2034605/range2034605/1
type: Range
persistent identity: range2034605
display id: range2034605
version: 1
top level: BBa_K208003
direction: direction/forward
start: 1
end: 3
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation2034604
display id: annotation2034604
version: 1
top level: BBa_K208003
location: https://synbiohub.org/public/igem/BBa_K208003/annotation2034604/range2034604/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K208003/annotation2034604/range2034604/1
type: Range
persistent identity: range2034604
display id: range2034604
version: 1
top level: BBa_K208003
direction: direction/forward
start: 1
end: 63
orientation: inline

https://synbiohub.org/public/igem/BBa_K336061_sequence/1
type: Sequence
persistent identity: BBa K336061 sequence
display id: BBa_K336061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggcctactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732005_sequence/1
type: Sequence
persistent identity: BBa I732005 sequence
display id: BBa_I732005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtggcgctggatggtaagccgctggcaagcgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K156010_sequence/1
type: Sequence
persistent identity: BBa K156010 sequence
display id: BBa_K156010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttagcaaaggtgaagagctgtttactggtgttgttccgatcctggttgaactggacggtgatgttaacggccacaagttctccgtgtctggcgaaggcgagggcgatgctacctacggtaaactgaccctgaagttcatctgtaccactggtaaactgccggtaccgtggccgaccctggttaccaccctgagccatggtgttcagtgttttgcacgttacccggatcacatgaagcagcatgatttcttcaagtctgcgatgccggagggttacgtccaggaacgcactatcttcttcaaagacgacggcaactataaaacccgtgccgaggttaagtttgagggtgacaccctggtcaaccgtattgaactgaaaggtatcgacttcaaagaagacggcaacattctgggtcacaaactggagtacaacttcaacagccataacgtgtacatcaccgccgataaacagaagaacggcatcaaagctaacttcaagattcgtcacaacatcgaagatggtggtgttcaactggctgatcactaccagcagaacacgccgatcggtgatggcccggtactgctgcctgacaaccattatctgtctacccagtctaaactgagcaaagacccgaatgagaaacgtgatcacatggtgctgctggagttcgtgactgcggcaggtatcacgctgggtatggacgaactgtataaatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K208017_sequence/1
type: Sequence
persistent identity: BBa K208017 sequence
display id: BBa_K208017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K208003_sequence/1
type: Sequence
persistent identity: BBa K208003 sequence
display id: BBa_K208003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggcc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z