##gff-version 3
##sequence-region BBa_K336100 1 860
BBa_K336100	.	terminator	820	860	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K336100	.	polyA_site	847	860	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K336100	.	stem_loop	827	846	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K336100	.	engineered_region	820	860	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K336100	.	stop_codon	853	853	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K336100	.	terminator	732	811	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K336100	.	stem_loop	743	786	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K336100	.	engineered_region	732	811	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K336100	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K336100	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K336100	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K336100	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K336100	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K336100	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K336100	.	CDS	81	698	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K336100	.	engineered_tag	699	723	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K336100	.	engineered_region	81	698	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K336100	.	CDS	81	659	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K336100	.	tag	660	691	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K336100	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K336100	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K336100
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttat
caagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatg
cagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatat
gctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgtttt
gctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaac
acgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagt
tccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaat
gaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgct
agtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata