LOCUS BBa_K336101 2764 bp DNA linear 22-Dec-2024 DEFINITION p(tetR)+α-amylase+luxR+lux reciever+ccdB ACCESSION BBa_K336101 VERSION BBa_K336101.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 misc_feature 1007..1025 /label=TetR 1 misc_feature 1007..1060 /label=BBa_R0040 promoter 1026..1031 /label=-35 misc_feature 1032..1050 /label=TetR 2 promoter 1049..1054 /label=-10 promoter 1007..1060 /label=BBa_R0040 misc_feature 1073..1076 /label=conserved RBS 1069..1080 /label=BBa_B0034 misc_feature 1087..2095 /label=BBa_K336012 CDS 1087..2095 /label=BBa_K336012 misc_feature 1069..2095 /label=BBa_K336013 stem_loop 2115..2158 /label=stem_loop misc_feature 2104..2183 /label=BBa_B0010 terminator 2104..2183 /label=BBa_B0010 stem_loop 2199..2218 /label=T7 TE misc_feature 2225..2225 /label=stop polyA_site 2219..2232 /label=polya misc_feature 2192..2232 /label=BBa_B0012 terminator 2192..2232 /label=BBa_B0012 misc_feature 1007..2232 /label=BBa_K336015 misc_feature 2260..2265 /label=-35 Region misc_feature 2282..2287 /label=-10 Region misc_feature 2241..2260 /label=LuxR promoter 2241..2295 /label=BBa_K091156 misc_feature 2308..2311 /label=conserved RBS 2304..2315 /label=BBa_B0034 misc_feature 2322..2324 /label=start misc_feature 2625..2627 /label=stop CDS 2322..2624 /label=cds CDS 2322..2627 /label=BBa_K145151 stem_loop 2647..2690 /label=stem_loop misc_feature 2636..2715 /label=BBa_B0010 terminator 2636..2715 /label=BBa_B0010 stem_loop 2731..2750 /label=T7 TE misc_feature 2757..2757 /label=stop polyA_site 2751..2764 /label=polya misc_feature 2724..2764 /label=BBa_B0012 terminator 2724..2764 /label=BBa_B0012 misc_feature 2241..2764 /label=BBa_K336016 misc_feature 1007..2764 /label=BBa_K336017 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat 1021 agagattgac atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa 1081 tactagatgt tcatggtgtt ttgatcattt taaattttta tatggcgggt ggtgggcaac 1141 tcgcttgcgc gggcaactcg cttaccgatt acgttagggc tgatatttac gtaaaaatcg 1201 tcaagggatg caagaccaaa gtagtaaaac cccggagtca acagcatcca agcccaagtc 1261 cttcacggag aaaccccagc gtccacatca cgagcgaagg accacctcta ggcatcggac 1321 gcaccatcca attagaagca gcaaagcgaa acagcccaag aaaaaggtcg gcccgtcggc 1381 cttttctgca acgctgatca cgggcagcga tccaaccaac accctccaga gtgactaggg 1441 gcggaaattt aaagggatta atttccactc aaccacaaat cacagtcgtc cccggtattg 1501 tcctgcagaa tgcaatttaa actcttctgc gaatcgcttg gattccccgc ccctggccgt 1561 agagcttaaa gtatgtccct tgtcgatgcg atgtatcaca acatataaat actagcaagg 1621 gatgccatgc ttggaggata gcaaccgaca acatcacatc aagctctccc ttctctgaac 1681 aataaacccc acagaaggca tttatgatgg tcgcgtggtg gtctctattt ctgtacggcc 1741 ttcaggtcgc ggcacctgct ttggctgcaa cgcctgcgga ctggcgatcg caatccattt 1801 atttccttct cacggatcga tttgcaagga cggatgggtc gacgactgcg acttgtaata 1861 ctgcggatca ggtgtgttgt tacctactag ctttcagaaa gaggaatgta aactgacttg 1921 atatagaaat actgtggtgg aacatggcag ggcatcatcg acaaggtaaa ttgccccttt 1981 atcaaaaaaa aagaaggaaa agcagaagaa aaataaaata aaaagaactc tagtcctaac 2041 catcacatag ttggactata tccagggaat gggcttcaca gccatctggt aataatacta 2101 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 2161 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 2221 tctgcgttta tatactagag acctgtagga tcgtacaggt ttaatcaaga aaatggtttg 2281 ttatagtcga ataaatacta gagaaagagg agaaatacta gatgcagttt aaggtttaca 2341 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca 2401 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct 2461 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg 2521 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg 2581 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataatac tagagccagg 2641 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2701 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2761 tata //