BBa_K337000 1 BBa_K337000 hsa-miR-122 Binding site pattern (3BS) 2010-10-20T11:00:00Z 2015-05-08T01:12:05Z http://mirbase.org/cgi-bin/mature.pl?mature_acc=MIMAT0000421 hsa-miR-122 binding site pattern, consisting of 3 perfect matching binding sites. The binding sites are seperated by sequences of 30 base pairs, which are innert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to standard restriction sites. false false _461_ 0 7259 9 It's complicated false The spacer sequences, present between the miRNA binding sites, should have the lowest compatibility to known miRNAs, to minimize unspecific sequence recognition. false Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R annotation2091163 1 Spacer 1st half range2091163 1 1 15 annotation2091165 1 Spacer 1st half range2091165 1 105 119 annotation2091168 1 Spacer 2nd half range2091168 1 90 104 annotation2091164 1 Spacer 1st half range2091164 1 53 67 annotation2091167 1 Spacer 2nd half range2091167 1 38 52 annotation2091169 1 Spacer 2nd half range2091169 1 142 156 BBa_K337000_sequence 1 cactgaatccaactgcaaacaccattgtcacactccagcatacatggactgccactgaatccaactgcaaacaccattgtcacactccagcatacatggactgccactgaatccaactgcaaacaccattgtcacactccagcatacatggactgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z