BBa_K337003 1 BBa_K337003 hsa-miR-122 Binding site pattern (4BS) 2010-10-20T11:00:00Z 2015-05-08T01:12:06Z http://mirbase.org/cgi-bin/mature.pl?mature_acc=MIMAT0000421 hsa-miR-122 binding site pattern, consisting of 4 binding sites (BS). The BS are seperated by sequences of 30 base pairs, which are innert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to standard restriction sites. Mutations showed up for BS 1 and 2, where BS 3 and 4 are perfectly matching: 1st: CAAACACCATTGTCACACTC-A perfect: CAAACACCATTGTCACACTCCA 2nd: CAAACACCATTGTCA-ACTCCA perfect: CAAACACCATTGTCACACTCCA false false _461_ 0 7259 9 It's complicated false The spacer sequences, present between the miRNA binding sites, should have the lowest compatibility to known miRNAs, to minimize unspecific sequence recognition. false Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R annotation2091205 1 Spacer 1st half range2091205 1 1 15 annotation2091207 1 Spacer 1st half range2091207 1 103 117 annotation2091209 1 Spacer 2nd half range2091209 1 37 51 annotation2091211 1 Spacer 2nd half range2091211 1 140 154 annotation2091212 1 Spacer 2nd half range2091212 1 192 206 annotation2091210 1 Spacer 1st half range2091210 1 155 169 annotation2091206 1 Spacer 1st half range2091206 1 52 66 annotation2091208 1 Spacer 2nd half range2091208 1 88 102 BBa_K337003_sequence 1 cactgaatccaactgcaaacaccattgtcacactcagcatacatggactgccactgaatccaactgcaaacaccattgtcaactccagcatacatggactgccactgaatccaactgcaaacaccattgtcacactccagcatacatggactgccactgaatccaactgcaaacaccattgtcacactccagcatacatggactgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z