BBa_K337003
1
BBa_K337003
hsa-miR-122 Binding site pattern (4BS)
2010-10-20T11:00:00Z
2015-05-08T01:12:06Z
http://mirbase.org/cgi-bin/mature.pl?mature_acc=MIMAT0000421
hsa-miR-122 binding site pattern, consisting of 4 binding sites (BS). The BS are seperated by sequences of 30 base pairs, which are innert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to standard restriction sites.
Mutations showed up for BS 1 and 2, where BS 3 and 4 are perfectly matching:
1st: CAAACACCATTGTCACACTC-A
perfect: CAAACACCATTGTCACACTCCA
2nd: CAAACACCATTGTCA-ACTCCA
perfect: CAAACACCATTGTCACACTCCA
false
false
_461_
0
7259
9
It's complicated
false
The spacer sequences, present between the miRNA binding sites, should have the lowest compatibility to known miRNAs, to minimize unspecific sequence recognition.
false
Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R
annotation2091205
1
Spacer 1st half
range2091205
1
1
15
annotation2091207
1
Spacer 1st half
range2091207
1
103
117
annotation2091209
1
Spacer 2nd half
range2091209
1
37
51
annotation2091211
1
Spacer 2nd half
range2091211
1
140
154
annotation2091212
1
Spacer 2nd half
range2091212
1
192
206
annotation2091210
1
Spacer 1st half
range2091210
1
155
169
annotation2091206
1
Spacer 1st half
range2091206
1
52
66
annotation2091208
1
Spacer 2nd half
range2091208
1
88
102
BBa_K337003_sequence
1
cactgaatccaactgcaaacaccattgtcacactcagcatacatggactgccactgaatccaactgcaaacaccattgtcaactccagcatacatggactgccactgaatccaactgcaaacaccattgtcacactccagcatacatggactgccactgaatccaactgcaaacaccattgtcacactccagcatacatggactgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z