LOCUS BBa_K337032 5244 bp DNA linear 09-Jan-2025 DEFINITION pSMB_miTuner Plasmid HD1 (BGH(rc)/shRNA10(rc)/sv40(rc)/RSV/Luc2_sv4 0/CMV/Kozag_hRluc_BGH ) ACCESSION BBa_K337032 VERSION BBa_K337032.1 FEATURES Location/Qualifiers misc_feature 1..255 /label=BGH Terminator misc_feature 261..421 /label=synthetic microRNA promoter 427..846 /label=SV40 promoter 851..1471 /label=RSV CDS 1477..3426 /label=Luc2 promoter 3432..4041 /label=CMV CDS 4047..4989 /label=hRluc misc_feature 4995..5244 /label=BGH Terminator ORIGIN 1 ggatccccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc 61 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg 121 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg 181 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg 241 atcagcgggt ttaaagctag taagcaaacg atgccaagac atttatcgag ggaaggattg 301 cctagcagta gctaggtacc acggtcaccg atattaatag tttagacata atatcggtgg 361 ccgtggtgtc cagctctgct aaggaaactc tgtagccacg aaggtgttaa cttcacctcc 421 agctagtaag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga 481 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat 541 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg 601 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc 661 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc 721 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agctggttct 781 ttccgcctca gaaggtacct aaccaagttc ctctttcaga ggttatttca ggccatggtg 841 ctgcgcgcta gtcaattcat gtttgacagc ttatcatcgc agatccgtat ggtgcactct 901 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt 961 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga 1021 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc 1081 cagatatacg cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt 1141 gtacgcggtt aggagtcccc tcaggatata gtagtttcgc ttttgcatag ggagggggaa 1201 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat 1261 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat 1321 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaatac 1381 cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca 1441 ttcaccacat tggtgtgcac ctccaagctg ggctagtgcc accatggaag atgccaaaaa 1501 cattaagaag ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct 1561 gcacaaagcc atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca 1621 tatcgaggtg gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc 1681 tatgaagcgc tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt 1741 gcagttcttc atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa 1801 cgacatctac aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt 1861 attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat 1921 acaaaagatc atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac 1981 cttcgtgact tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt 2041 cgaccgggac aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa 2101 gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat 2161 cttcggcaac cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg 2221 cttcggcatg ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta 2281 ccgcttcgag gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct 2341 gctggtgccc acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct 2401 aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc 2461 cgtggccaaa cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac 2521 cagcgccatt ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt 2581 gcccttcttc gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca 2641 gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga 2701 ggctacaaac gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg 2761 ggacgaggac gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg 2821 ctaccaggta gccccagccg aactggagag catcctgctg caacacccca acatcttcga 2881 cgccggggtc gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt 2941 gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt 3001 tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact 3061 gaccggcaag ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg 3121 caagatcgcc gtgtaataat tctagccccg gggcggtcag aactcgaggc tagagtcggg 3181 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa 3241 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg 3301 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc 3361 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta 3421 ggatccgcta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 3481 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 3541 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 3601 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 3661 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 3721 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 3781 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 3841 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 3901 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 3961 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact 4021 gcttactggc ttatcgaaat ggctagtgcc accatggctt ccaaggtgta cgaccccgag 4081 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg 4141 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt 4201 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc 4261 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat 4321 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac 4381 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac 4441 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg 4501 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa 4561 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag 4621 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag 4681 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc 4741 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat 4801 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga 4861 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag 4921 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac 4981 gagcagtaag ctagttttaa acccgctgat cagcctcgac tgtgccttct agttgccagc 5041 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 5101 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 5161 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 5221 ctggggatgc ggtgggctct atgg //