LOCUS BBa_K337036 5436 bp DNA linear 22-Nov-2024 DEFINITION pSMB_miTuner Plasmid HD3 (BGH(rc)/shRNA10(rc)/RSV(rc)/CMV/Luc2_sv40 /CMV/Kozag_hRluc_BGH ) ACCESSION BBa_K337036 VERSION BBa_K337036.1 FEATURES Location/Qualifiers misc_feature 1..255 /label=BGH Terminator misc_feature 261..421 /label=synthetic microRNA promoter 427..1048 /label=RSV promoter 1054..1663 /label=CMV misc_feature 1669..3618 /label=Luc2_SV40 promoter 3618..4233 /label=CMV misc_feature 4239..5181 /label=hRluc misc_feature 5187..5436 /label=BGH Terminator ORIGIN 1 ggatccccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc 61 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg 121 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg 181 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg 241 atcagcgggt ttaaagctag taagcaaacg atgccaagac atttatcgag ggaaggattg 301 cctagcagta gctaggtacc acggtcaccg atattaatag tttagacata atatcggtgg 361 ccgtggtgtc cagctctgct aaggaaactc tgtagccacg aaggtgttaa cttcacctcc 421 agctagtcag cttggaggtg cacaccaatg tggtgaatgg tcaaatggcg tttattgtat 481 cgagctaggc acttaaatac aatatctctg caatgcggta ttcagtggtt cgtccaatcc 541 atgtcagacc cgtctgttgc cttcctaata aggcacgatc gtaccacctt acttccacca 601 atcggcatgc acggtgcttt ttctctcctt gtaaggcatg ttgctaactc atcgttacca 661 tgttgcaaga ctacaagagt attgcataag actacatttc cccctcccta tgcaaaagcg 721 aaactactat atcctgaggg gactcctaac cgcgtacaac cgaagccccg cttttcgcct 781 aaacacaccc tagtcccctc agatacgcgt atatctggcc cgtacatcgc gaagcagcgc 841 aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt gccttgttgt 901 agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag ggagcagata 961 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata cggatctgcg 1021 atgataagct gtcaaacatg agaattgggc tagttattaa tagtaatcaa ttacggggtc 1081 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 1141 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 1201 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 1261 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 1321 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 1381 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa 1441 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa 1501 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc 1561 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct 1621 ctggctaact agagaaccca ctgcttactg gcttatcgaa atggctagtg ccaccatgga 1681 agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc 1741 cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt 1801 taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg 1861 gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag 1921 cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt 1981 ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca 2041 gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa 2101 gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca 2161 aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt 2221 gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac 2281 cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc 2341 ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc 2401 atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt 2461 cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat 2521 tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga 2581 caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga 2641 ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct 2701 gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt 2761 aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact 2821 gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt 2881 taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga 2941 catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat 3001 caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc 3061 caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc 3121 cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt 3181 ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt 3241 gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc 3301 caagaagggc ggcaagatcg ccgtgtaata attctagccc cggggcggtc agaactcgag 3361 gctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg 3421 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta 3481 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc 3541 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct 3601 acaaatgtgg taggatccgc tagttattaa tagtaatcaa ttacggggtc attagttcat 3661 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 3721 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 3781 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 3841 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 3901 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 3961 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 4021 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 4081 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 4141 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact 4201 agagaaccca ctgcttactg gcttatcgaa atggctagtg ccaccatggc ttccaaggtg 4261 tacgaccccg agcaacgcaa acgcatgatc actgggcctc agtggtgggc tcgctgcaag 4321 caaatgaacg tgctggactc cttcatcaac tactatgatt ccgagaagca cgccgagaac 4381 gccgtgattt ttctgcatgg taacgctgcc tccagctacc tgtggaggca cgtcgtgcct 4441 cacatcgagc ccgtggctag atgcatcatc cctgatctga tcggaatggg taagtccggc 4501 aagagcggga atggctcata tcgcctcctg gatcactaca agtacctcac cgcttggttc 4561 gagctgctga accttccaaa gaaaatcatc tttgtgggcc acgactgggg ggcttgtctg 4621 gcctttcact actcctacga gcaccaagac aagatcaagg ccatcgtcca tgctgagagt 4681 gtcgtggacg tgatcgagtc ctgggacgag tggcctgaca tcgaggagga tatcgccctg 4741 atcaagagcg aagagggcga gaaaatggtg cttgagaata acttcttcgt cgagaccatg 4801 ctcccaagca agatcatgcg gaaactggag cctgaggagt tcgctgccta cctggagcca 4861 ttcaaggaga agggcgaggt tagacggcct accctctcct ggcctcgcga gatccctctc 4921 gttaagggag gcaagcccga cgtcgtccag attgtccgca actacaacgc ctaccttcgg 4981 gccagcgacg atctgcctaa gatgttcatc gagtccgacc ctgggttctt ttccaacgct 5041 attgtcgagg gagctaagaa gttccctaac accgagttcg tgaaggtgaa gggcctccac 5101 ttcagccagg aggacgctcc agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc 5161 gtgctgaaga acgagcagta agctagtttt aaacccgctg atcagcctcg actgtgcctt 5221 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 5281 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 5341 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 5401 atagcaggca tgctggggat gcggtgggct ctatgg //