LOCUS BBa_K337038 5557 bp DNA linear 23-Dec-2024 DEFINITION pSMB_miTuner Plasmid HD4 (BGH(rc)/shRNA10(rc)/RSV(rc)/CMV_TetO2/Luc 2_sv40/CMV/Kozag_hRluc_BGH) ACCESSION BBa_K337038 VERSION BBa_K337038.1 FEATURES Location/Qualifiers misc_feature 1..255 /label=BGH Terminator promoter 427..1048 /label=RSV misc_feature 261..421 /label=synthetic microRNA promoter 1055..1784 /label=CMV_TetO2 misc_feature 1790..3739 /label=Luc2_SV40 promoter 3745..4354 /label=CMV misc_feature 4360..5302 /label=hRluc misc_feature 5308..5557 /label=BHG Terminator ORIGIN 1 ggatccccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc 61 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg 121 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg 181 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg 241 atcagcgggt ttaaagctag taagcaaacg atgccaagac atttatcgag ggaaggattg 301 cctagcagta gctaggtacc acggtcaccg atattaatag tttagacata atatcggtgg 361 ccgtggtgtc cagctctgct aaggaaactc tgtagccacg aaggtgttaa cttcacctcc 421 agctagtcag cttggaggtg cacaccaatg tggtgaatgg tcaaatggcg tttattgtat 481 cgagctaggc acttaaatac aatatctctg caatgcggta ttcagtggtt cgtccaatcc 541 atgtcagacc cgtctgttgc cttcctaata aggcacgatc gtaccacctt acttccacca 601 atcggcatgc acggtgcttt ttctctcctt gtaaggcatg ttgctaactc atcgttacca 661 tgttgcaaga ctacaagagt attgcataag actacatttc cccctcccta tgcaaaagcg 721 aaactactat atcctgaggg gactcctaac cgcgtacaac cgaagccccg cttttcgcct 781 aaacacaccc tagtcccctc agatacgcgt atatctggcc cgtacatcgc gaagcagcgc 841 aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt gccttgttgt 901 agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag ggagcagata 961 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata cggatctgcg 1021 atgataagct gtcaaacatg agaattgggc tagtgttgac attgattatt gtctagttat 1081 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 1141 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 1201 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 1261 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 1321 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 1381 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 1441 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 1501 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 1561 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 1621 gaggtctata taagcagagc tctccctatc agtgatagag atctccctat cagtgataga 1681 gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt 1741 tttgacctcc atagaagaca ccgggaccga tccagcctcc ggtggctagt gccaccatgg 1801 aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg 1861 ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct 1921 ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc 1981 ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca 2041 gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg 2101 tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc 2161 agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga 2221 agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc 2281 aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg 2341 tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta 2401 ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg 2461 cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc 2521 catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg 2581 tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga 2641 ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg 2701 acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg 2761 aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc 2821 tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag 2881 taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac 2941 tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg 3001 ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg 3061 acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga 3121 tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc 3181 ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg 3241 ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg 3301 tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg 3361 tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg 3421 ccaagaaggg cggcaagatc gccgtgtaat aattctagcc ccggggcggt cagaactcga 3481 ggctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt 3541 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct 3601 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt 3661 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc 3721 tacaaatgtg gtaggatccg ctagttatta atagtaatca attacggggt cattagttca 3781 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 3841 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 3901 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 3961 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 4021 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 4081 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 4141 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 4201 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 4261 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac 4321 tagagaaccc actgcttact ggcttatcga aatggctagt gccaccatgg cttccaaggt 4381 gtacgacccc gagcaacgca aacgcatgat cactgggcct cagtggtggg ctcgctgcaa 4441 gcaaatgaac gtgctggact ccttcatcaa ctactatgat tccgagaagc acgccgagaa 4501 cgccgtgatt tttctgcatg gtaacgctgc ctccagctac ctgtggaggc acgtcgtgcc 4561 tcacatcgag cccgtggcta gatgcatcat ccctgatctg atcggaatgg gtaagtccgg 4621 caagagcggg aatggctcat atcgcctcct ggatcactac aagtacctca ccgcttggtt 4681 cgagctgctg aaccttccaa agaaaatcat ctttgtgggc cacgactggg gggcttgtct 4741 ggcctttcac tactcctacg agcaccaaga caagatcaag gccatcgtcc atgctgagag 4801 tgtcgtggac gtgatcgagt cctgggacga gtggcctgac atcgaggagg atatcgccct 4861 gatcaagagc gaagagggcg agaaaatggt gcttgagaat aacttcttcg tcgagaccat 4921 gctcccaagc aagatcatgc ggaaactgga gcctgaggag ttcgctgcct acctggagcc 4981 attcaaggag aagggcgagg ttagacggcc taccctctcc tggcctcgcg agatccctct 5041 cgttaaggga ggcaagcccg acgtcgtcca gattgtccgc aactacaacg cctaccttcg 5101 ggccagcgac gatctgccta agatgttcat cgagtccgac cctgggttct tttccaacgc 5161 tattgtcgag ggagctaaga agttccctaa caccgagttc gtgaaggtga agggcctcca 5221 cttcagccag gaggacgctc cagatgaaat gggtaagtac atcaagagct tcgtggagcg 5281 cgtgctgaag aacgagcagt aagctagttt taaacccgct gatcagcctc gactgtgcct 5341 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt 5401 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg 5461 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac 5521 aatagcaggc atgctgggga tgcggtgggc tctatgg //