LOCUS BBa_K337040 5254 bp DNA linear 27-Dec-2024 DEFINITION pSMB_miTuner Plasmid HD5 (BGH(rc)/shRNA10(rc)/sv40(rc)/RSV/Luc2_sv4 0/RSV/Kozag_hRluc_BGH) ACCESSION BBa_K337040 VERSION BBa_K337040.1 FEATURES Location/Qualifiers misc_feature 1..255 /label=BGH Terminator misc_feature 261..421 /label=synthetic microRNA promoter 427..846 /label=SV40 promoter 852..1471 /label=RSV CDS 1477..3426 /label=Luc2 promoter 3432..4051 /label=RSV CDS 4057..4999 /label=hRluc misc_feature 5005..5254 /label=BGH Terminator ORIGIN 1 ggatccccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc 61 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg 121 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg 181 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg 241 atcagcgggt ttaaagctag taagcaaacg atgccaagac atttatcgag ggaaggattg 301 cctagcagta gctaggtacc acggtcaccg atattaatag tttagacata atatcggtgg 361 ccgtggtgtc cagctctgct aaggaaactc tgtagccacg aaggtgttaa cttcacctcc 421 agctagtaag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga 481 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat 541 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg 601 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc 661 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc 721 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agctggttct 781 ttccgcctca gaaggtacct aaccaagttc ctctttcaga ggttatttca ggccatggtg 841 ctgcgcgcta gtcaattcat gtttgacagc ttatcatcgc agatccgtat ggtgcactct 901 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt 961 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga 1021 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc 1081 cagatatacg cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt 1141 gtacgcggtt aggagtcccc tcaggatata gtagtttcgc ttttgcatag ggagggggaa 1201 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat 1261 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat 1321 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaatac 1381 cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca 1441 ttcaccacat tggtgtgcac ctccaagctg ggctagtgcc accatggaag atgccaaaaa 1501 cattaagaag ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct 1561 gcacaaagcc atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca 1621 tatcgaggtg gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc 1681 tatgaagcgc tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt 1741 gcagttcttc atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa 1801 cgacatctac aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt 1861 attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat 1921 acaaaagatc atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac 1981 cttcgtgact tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt 2041 cgaccgggac aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa 2101 gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat 2161 cttcggcaac cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg 2221 cttcggcatg ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta 2281 ccgcttcgag gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct 2341 gctggtgccc acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct 2401 aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc 2461 cgtggccaaa cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac 2521 cagcgccatt ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt 2581 gcccttcttc gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca 2641 gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga 2701 ggctacaaac gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg 2761 ggacgaggac gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg 2821 ctaccaggta gccccagccg aactggagag catcctgctg caacacccca acatcttcga 2881 cgccggggtc gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt 2941 gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt 3001 tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact 3061 gaccggcaag ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg 3121 caagatcgcc gtgtaataat tctagccccg gggcggtcag aactcgaggc tagagtcggg 3181 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa 3241 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg 3301 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc 3361 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta 3421 ggatccgcta gtcaattcat gtttgacagc ttatcatcgc agatccgtat ggtgcactct 3481 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt 3541 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga 3601 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc 3661 cagatatacg cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt 3721 gtacgcggtt aggagtcccc tcaggatata gtagtttcgc ttttgcatag ggagggggaa 3781 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat 3841 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat 3901 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaatac 3961 cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca 4021 ttcaccacat tggtgtgcac ctccaagctg ggctagtgcc accatggctt ccaaggtgta 4081 cgaccccgag caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca 4141 aatgaacgtg ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc 4201 cgtgattttt ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca 4261 catcgagccc gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa 4321 gagcgggaat ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga 4381 gctgctgaac cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc 4441 ctttcactac tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt 4501 cgtggacgtg atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat 4561 caagagcgaa gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct 4621 cccaagcaag atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt 4681 caaggagaag ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt 4741 taagggaggc aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc 4801 cagcgacgat ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat 4861 tgtcgaggga gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt 4921 cagccaggag gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt 4981 gctgaagaac gagcagtaag ctagttttaa acccgctgat cagcctcgac tgtgccttct 5041 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc 5101 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt 5161 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat 5221 agcaggcatg ctggggatgc ggtgggctct atgg //