LOCUS BBa_K337042 5244 bp DNA linear 09-Jan-2025 DEFINITION pSMB_miTuner Plasmid HD6 (BGH(rc)/shRNA10(rc)/sv40(rc)/CMV/RSV/Luc2 _sv40/RSV/Kozag_hRluc_BGH) ACCESSION BBa_K337042 VERSION BBa_K337042.1 FEATURES Location/Qualifiers misc_feature 1..255 /label=BGH Terminator misc_feature 261..421 /label=synthetic microRNA promoter 852..1461 /label=CMV CDS 1467..3416 /label=Luc2 promoter 427..846 /label=SV40 promoter 3422..4041 /label=RSV CDS 4047..4989 /label=hRluc misc_feature 4995..5244 /label=BGH Terminator ORIGIN 1 ggatccccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc 61 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg 121 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg 181 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg 241 atcagcgggt ttaaagctag taagcaaacg atgccaagac atttatcgag ggaaggattg 301 cctagcagta gctaggtacc acggtcaccg atattaatag tttagacata atatcggtgg 361 ccgtggtgtc cagctctgct aaggaaactc tgtagccacg aaggtgttaa cttcacctcc 421 agctagtaag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga 481 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat 541 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg 601 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc 661 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc 721 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agctggttct 781 ttccgcctca gaaggtacct aaccaagttc ctctttcaga ggttatttca ggccatggtg 841 ctgcgcgcta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 901 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 961 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 1021 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 1081 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 1141 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 1201 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 1261 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 1321 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 1381 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact 1441 gcttactggc ttatcgaaat ggctagtgcc accatggaag atgccaaaaa cattaagaag 1501 ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc 1561 atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg 1621 gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc 1681 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc 1741 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac 1801 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc 1861 aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc 1921 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact 1981 tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac 2041 aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc 2101 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac 2161 cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg 2221 ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag 2281 gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc 2341 acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg 2401 cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa 2461 cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt 2521 ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc 2581 gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag 2641 ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac 2701 gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac 2761 gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta 2821 gccccagccg aactggagag catcctgctg caacacccca acatcttcga cgccggggtc 2881 gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac 2941 ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc 3001 aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag 3061 ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc 3121 gtgtaataat tctagccccg gggcggtcag aactcgaggc tagagtcggg gcggccggcc 3181 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 3241 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 3301 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 3361 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta ggatccgcta 3421 gtcaattcat gtttgacagc ttatcatcgc agatccgtat ggtgcactct cagtacaatc 3481 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct 3541 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg 3601 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg 3661 cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt gtacgcggtt 3721 aggagtcccc tcaggatata gtagtttcgc ttttgcatag ggagggggaa atgtagtctt 3781 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag 3841 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat 3901 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaatac cgcattgcag 3961 agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca ttcaccacat 4021 tggtgtgcac ctccaagctg ggctagtgcc accatggctt ccaaggtgta cgaccccgag 4081 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg 4141 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt 4201 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc 4261 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat 4321 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac 4381 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac 4441 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg 4501 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa 4561 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag 4621 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag 4681 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc 4741 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat 4801 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga 4861 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag 4921 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac 4981 gagcagtaag ctagttttaa acccgctgat cagcctcgac tgtgccttct agttgccagc 5041 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 5101 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 5161 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 5221 ctggggatgc ggtgggctct atgg //