LOCUS BBa_K337044 5446 bp DNA linear 26-Nov-2024 DEFINITION pSMB_miTuner Plasmid HD7 (BGH(rc)/shRNA10(rc)/RSV(rc)/CMV/Luc2_sv40 /RSV/Kozag_hRluc_BGH ) ACCESSION BBa_K337044 VERSION BBa_K337044.1 FEATURES Location/Qualifiers misc_feature 1..255 /label=BGH Terminator misc_feature 261..421 /label=synthetic microRNA CDS 1669..3618 /label=Luc2 promoter 427..1048 /label=RSV promoter 1054..1663 /label=CMV CDS 3624..4243 /label=RSV CDS 4249..5191 /label=hRluc misc_feature 5197..5446 /label=BGH Terminator ORIGIN 1 ggatccccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc 61 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg 121 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg 181 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg 241 atcagcgggt ttaaagctag taagcaaacg atgccaagac atttatcgag ggaaggattg 301 cctagcagta gctaggtacc acggtcaccg atattaatag tttagacata atatcggtgg 361 ccgtggtgtc cagctctgct aaggaaactc tgtagccacg aaggtgttaa cttcacctcc 421 agctagtcag cttggaggtg cacaccaatg tggtgaatgg tcaaatggcg tttattgtat 481 cgagctaggc acttaaatac aatatctctg caatgcggta ttcagtggtt cgtccaatcc 541 atgtcagacc cgtctgttgc cttcctaata aggcacgatc gtaccacctt acttccacca 601 atcggcatgc acggtgcttt ttctctcctt gtaaggcatg ttgctaactc atcgttacca 661 tgttgcaaga ctacaagagt attgcataag actacatttc cccctcccta tgcaaaagcg 721 aaactactat atcctgaggg gactcctaac cgcgtacaac cgaagccccg cttttcgcct 781 aaacacaccc tagtcccctc agatacgcgt atatctggcc cgtacatcgc gaagcagcgc 841 aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt gccttgttgt 901 agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag ggagcagata 961 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata cggatctgcg 1021 atgataagct gtcaaacatg agaattgggc tagttattaa tagtaatcaa ttacggggtc 1081 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 1141 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 1201 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 1261 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 1321 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 1381 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa 1441 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa 1501 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc 1561 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct 1621 ctggctaact agagaaccca ctgcttactg gcttatcgaa atggctagtg ccaccatgga 1681 agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc 1741 cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt 1801 taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg 1861 gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag 1921 cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt 1981 ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca 2041 gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa 2101 gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca 2161 aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt 2221 gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac 2281 cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc 2341 ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc 2401 atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt 2461 cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat 2521 tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga 2581 caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga 2641 ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct 2701 gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt 2761 aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact 2821 gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt 2881 taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga 2941 catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat 3001 caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc 3061 caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc 3121 cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt 3181 ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt 3241 gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc 3301 caagaagggc ggcaagatcg ccgtgtaata attctagccc cggggcggtc agaactcgag 3361 gctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg 3421 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta 3481 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc 3541 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct 3601 acaaatgtgg taggatccgc tagtcaattc atgtttgaca gcttatcatc gcagatccgt 3661 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc 3721 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca 3781 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg 3841 cgatgtacgg gccagatata cgcgtatctg aggggactag ggtgtgttta ggcgaaaagc 3901 ggggcttcgg ttgtacgcgg ttaggagtcc cctcaggata tagtagtttc gcttttgcat 3961 agggaggggg aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg 4021 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta 4081 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga 4141 accactgaat accgcattgc agagatattg tatttaagtg cctagctcga tacaataaac 4201 gccatttgac cattcaccac attggtgtgc acctccaagc tgggctagtg ccaccatggc 4261 ttccaaggtg tacgaccccg agcaacgcaa acgcatgatc actgggcctc agtggtgggc 4321 tcgctgcaag caaatgaacg tgctggactc cttcatcaac tactatgatt ccgagaagca 4381 cgccgagaac gccgtgattt ttctgcatgg taacgctgcc tccagctacc tgtggaggca 4441 cgtcgtgcct cacatcgagc ccgtggctag atgcatcatc cctgatctga tcggaatggg 4501 taagtccggc aagagcggga atggctcata tcgcctcctg gatcactaca agtacctcac 4561 cgcttggttc gagctgctga accttccaaa gaaaatcatc tttgtgggcc acgactgggg 4621 ggcttgtctg gcctttcact actcctacga gcaccaagac aagatcaagg ccatcgtcca 4681 tgctgagagt gtcgtggacg tgatcgagtc ctgggacgag tggcctgaca tcgaggagga 4741 tatcgccctg atcaagagcg aagagggcga gaaaatggtg cttgagaata acttcttcgt 4801 cgagaccatg ctcccaagca agatcatgcg gaaactggag cctgaggagt tcgctgccta 4861 cctggagcca ttcaaggaga agggcgaggt tagacggcct accctctcct ggcctcgcga 4921 gatccctctc gttaagggag gcaagcccga cgtcgtccag attgtccgca actacaacgc 4981 ctaccttcgg gccagcgacg atctgcctaa gatgttcatc gagtccgacc ctgggttctt 5041 ttccaacgct attgtcgagg gagctaagaa gttccctaac accgagttcg tgaaggtgaa 5101 gggcctccac ttcagccagg aggacgctcc agatgaaatg ggtaagtaca tcaagagctt 5161 cgtggagcgc gtgctgaaga acgagcagta agctagtttt aaacccgctg atcagcctcg 5221 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 5281 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 5341 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 5401 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatgg //