LOCUS BBa_K337046 5567 bp DNA linear 27-Dec-2024 DEFINITION pSMB_miTuner Plasmid HD8 (BGH(rc)/shRNA10(rc)/RSV(rc)/CMV_TetO2/Luc 2_sv40/RSV/Kozag_hRluc_BGH ) ACCESSION BBa_K337046 VERSION BBa_K337046.1 FEATURES Location/Qualifiers misc_feature 1..255 /label=BGH Terminator misc_feature 261..421 /label=synthetic microRNA promoter 427..1048 /label=RSV promoter 1054..1784 /label=CMV_TetO2 CDS 1790..3739 /label=Luc2 promoter 3745..4364 /label=RSV CDS 4370..5312 /label=hRluc misc_feature 5318..5567 /label=BGH Terminator ORIGIN 1 ggatccccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc 61 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg 121 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg 181 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg 241 atcagcgggt ttaaagctag taagcaaacg atgccaagac atttatcgag ggaaggattg 301 cctagcagta gctaggtacc acggtcaccg atattaatag tttagacata atatcggtgg 361 ccgtggtgtc cagctctgct aaggaaactc tgtagccacg aaggtgttaa cttcacctcc 421 agctagtcag cttggaggtg cacaccaatg tggtgaatgg tcaaatggcg tttattgtat 481 cgagctaggc acttaaatac aatatctctg caatgcggta ttcagtggtt cgtccaatcc 541 atgtcagacc cgtctgttgc cttcctaata aggcacgatc gtaccacctt acttccacca 601 atcggcatgc acggtgcttt ttctctcctt gtaaggcatg ttgctaactc atcgttacca 661 tgttgcaaga ctacaagagt attgcataag actacatttc cccctcccta tgcaaaagcg 721 aaactactat atcctgaggg gactcctaac cgcgtacaac cgaagccccg cttttcgcct 781 aaacacaccc tagtcccctc agatacgcgt atatctggcc cgtacatcgc gaagcagcgc 841 aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt gccttgttgt 901 agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag ggagcagata 961 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata cggatctgcg 1021 atgataagct gtcaaacatg agaattgggc tagtgttgac attgattatt gtctagttat 1081 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 1141 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 1201 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 1261 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 1321 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 1381 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 1441 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 1501 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 1561 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 1621 gaggtctata taagcagagc tctccctatc agtgatagag atctccctat cagtgataga 1681 gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt 1741 tttgacctcc atagaagaca ccgggaccga tccagcctcc ggtggctagt gccaccatgg 1801 aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg 1861 ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct 1921 ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc 1981 ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca 2041 gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg 2101 tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc 2161 agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga 2221 agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc 2281 aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg 2341 tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta 2401 ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg 2461 cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc 2521 catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg 2581 tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga 2641 ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg 2701 acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg 2761 aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc 2821 tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag 2881 taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac 2941 tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg 3001 ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg 3061 acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga 3121 tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc 3181 ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg 3241 ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg 3301 tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg 3361 tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg 3421 ccaagaaggg cggcaagatc gccgtgtaat aattctagcc ccggggcggt cagaactcga 3481 ggctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt 3541 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct 3601 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt 3661 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc 3721 tacaaatgtg gtaggatccg ctagtcaatt catgtttgac agcttatcat cgcagatccg 3781 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc 3841 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc 3901 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc 3961 gcgatgtacg ggccagatat acgcgtatct gaggggacta gggtgtgttt aggcgaaaag 4021 cggggcttcg gttgtacgcg gttaggagtc ccctcaggat atagtagttt cgcttttgca 4081 tagggagggg gaaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat 4141 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt 4201 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg 4261 aaccactgaa taccgcattg cagagatatt gtatttaagt gcctagctcg atacaataaa 4321 cgccatttga ccattcacca cattggtgtg cacctccaag ctgggctagt gccaccatgg 4381 cttccaaggt gtacgacccc gagcaacgca aacgcatgat cactgggcct cagtggtggg 4441 ctcgctgcaa gcaaatgaac gtgctggact ccttcatcaa ctactatgat tccgagaagc 4501 acgccgagaa cgccgtgatt tttctgcatg gtaacgctgc ctccagctac ctgtggaggc 4561 acgtcgtgcc tcacatcgag cccgtggcta gatgcatcat ccctgatctg atcggaatgg 4621 gtaagtccgg caagagcggg aatggctcat atcgcctcct ggatcactac aagtacctca 4681 ccgcttggtt cgagctgctg aaccttccaa agaaaatcat ctttgtgggc cacgactggg 4741 gggcttgtct ggcctttcac tactcctacg agcaccaaga caagatcaag gccatcgtcc 4801 atgctgagag tgtcgtggac gtgatcgagt cctgggacga gtggcctgac atcgaggagg 4861 atatcgccct gatcaagagc gaagagggcg agaaaatggt gcttgagaat aacttcttcg 4921 tcgagaccat gctcccaagc aagatcatgc ggaaactgga gcctgaggag ttcgctgcct 4981 acctggagcc attcaaggag aagggcgagg ttagacggcc taccctctcc tggcctcgcg 5041 agatccctct cgttaaggga ggcaagcccg acgtcgtcca gattgtccgc aactacaacg 5101 cctaccttcg ggccagcgac gatctgccta agatgttcat cgagtccgac cctgggttct 5161 tttccaacgc tattgtcgag ggagctaaga agttccctaa caccgagttc gtgaaggtga 5221 agggcctcca cttcagccag gaggacgctc cagatgaaat gggtaagtac atcaagagct 5281 tcgtggagcg cgtgctgaag aacgagcagt aagctagttt taaacccgct gatcagcctc 5341 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac 5401 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg 5461 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga 5521 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatgg //