BBa_K337057
1
BBa_K337057
synthetic binding site of miR 122 (imperfect) KD: 24%
2010-10-25T11:00:00Z
2015-05-08T01:12:06Z
synthetic
imperfect binding site of miR122 in the tuning construct leads to a knockdown percentage of 64% percent if introduced into the 3'UTR of the gene of interest (GOI). part can be either co-transfected with a synthetic miRNA or transfected into liver cell lines (i.e. Huh7).
false
false
_461_
0
7192
9
Not in stock
false
designed to lead to 30% KD if introduced into the 3'UTR of your GOI and transfected into liver cell lines or co-transfected with miR122.
false
Rebecca Berrens
annotation2104221
1
seed miR122
range2104221
1
15
22
annotation2104222
1
seed mismatch
range2104222
1
15
15
BBa_K337057_sequence
1
cccctgatgggggcgacactcca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z