BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K338004
1
BBa_K338004
PHA Synthase Composite, Part 2/2
2010-10-25T11:00:00Z
2015-05-08T01:12:07Z
Registry of Standard Biological Parts
This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: ''phaA'', ''phaB1'', ''phaC1''. This half contains ''phaB1'' and ''phaC1'': <partinfo>BBa_K156013</partinfo> & <partinfo>BBa_K156014</partinfo>. It was designed to be ligated downstream of part [[Team:Caltech/BBa_K338003|BBa_K338003]].
false
false
_454_
0
6013
9
It's complicated
true
When ligated downstream of [[Team:Caltech/BBa_K338003|BBa_K338003]], the completed construct was designed to express all three PHA synthase genes required to make PHB oligomers from soybean oil. The three genes would be transcribed polycistronically on a single mRNA transcript under the IPTG-inducible control of the <partinfo>BBa_K215000</partinfo> promoter. Naturally, each gene is preceded by a standard RBS (<partinfo>B0034</partinfo>) and the transcript finishes with a strong terminator (<partinfo>B0015</partinfo>), for a total size of about 3500bp.
Note that these three genes should only cause the production of PHB ''oligomers'' in cells, not hardened plastic. A crosslinking agent is required to link the oligomers and form the final plastic product. Over-expression of the ''phaC1'' gene could cause some crosslinking, but this has not been experimentally verified.
false
Lucas Hartsough
component2101496
1
BBa_K156013
component2101498
1
BBa_B0034
component2101506
1
BBa_B0015
component2101499
1
BBa_K156014
annotation2101496
1
BBa_K156013
range2101496
1
1
741
annotation2101499
1
BBa_K156014
range2101499
1
768
2116
annotation2101498
1
BBa_B0034
range2101498
1
750
761
annotation2101506
1
BBa_B0015
range2101506
1
2125
2253
BBa_K156013
1
Bioplastic
phaB1 (acetyacetyl-CoA reductase)
2008-10-28T12:00:00Z
2015-05-08T01:10:54Z
This coding region is native to Ralstonia eutropha H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031).
This part codes for an enzyme that catalyzes the formation of 3-hydroxybutyryl-CoA from acetoacetyl-CoA in polyhydroxyalkanoate synthesis.
false
false
_246_
0
1475
9
It's complicated
false
This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
false
George McArthur IV
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K156014
1
Bioplastic
phaC1 (Poly(3-hydroxybutyrate) polymerase)
2008-10-28T12:00:00Z
2015-05-08T01:10:54Z
This coding region is native to Ralstonia eutropha H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031).
This part codes for a polyhydroxybutyrate (PHB) synthase, which catalyzes the polymerization of hydroxybutyryl-CoA.
false
false
_246_
0
1475
9
It's complicated
false
This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
false
George McArthur IV
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K156013_sequence
1
atgactcaacgtatcgcatatgtaactggtggtatgggtggtatcggtactgcaatttgccagcgtctggcgaaagacggtttccgtgttgttgcgggctgcggtccgaactccccgcgtcgtgaaaagtggctggaacaacagaaagccctgggcttcgacttcattgcctccgagggtaatgtagctgactgggattccaccaagactgccttcgataaagttaaatctgaagtgggcgaagtagatgtactgatcaacaacgccggtattactcgtgatgtcgtattccgcaaaatgacccgtgcagactgggatgcagttatcgacaccaacctgacgtctctgttcaacgttaccaaacaggttattgatggtatggctgaccgtggctggggccgcatcgtgaacatctctagcgttaacggccaaaaaggccaatttggtcagacgaattacagcacggctaaagcaggcctgcacggtttcaccatggcactggcgcaggaagtggcgaccaaaggtgttaccgttaataccgtttctccaggttacatcgccaccgatatggttaaggctatccgccaagatgttctggacaagatcgtggctaccattccggttaaacgcctgggcctgccggaagaaattgcgtccatctgtgcgtggctgagctccgaagagtctggtttttccaccggtgcggatttctctctgaacggtggtctgcacatgggttga
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K156014_sequence
1
atggcaactggtaaaggtgctgctgcatctactcaagaaggtaaatcccagccgttcaaagtgacccctggtccgttcgatccggcgacctggctggaatggagccgccagtggcagggcactgaaggcaacggccacgcagctgcttctggcattccgggtctggacgctctggcgggcgtaaaaatcgcaccggcacaactgggcgacatccagcaacgttatatgaaagatttcagcgcgctgtggcaggcgatggcggaaggcaaagccgaagcgaccggtccgctgcacgaccgtcgtttcgcaggtgacgcgtggcgtaccaacctgccataccgtttcgctgccgccttttacctgctgaacgcacgtgcgctgaccgaactggctgatgcagttgaagctgacgccaaaacccgccagcgtatccgtttcgctatcagccagtgggtcgacgcgatgagcccggccaacttcctggccacgaacccggaggcccagcgcctgctgatcgaatctggtggtgaatctctgcgtgcgggcgttcgtaacatgatggaggatctgacgcgcggtaagatctctcaaaccgacgagagcgcgtttgaagttggccgtaacgtggcggttactgaaggcgcggttgttttcgagaacgaatatttccagctgctgcaatacaaaccgctgaccgacaaggttcacgctcgtccgctgctgatggtaccgccttgcatcaacaaatactacatcctggacctgcaaccagaatcctctctggttcgtcacgtggttgagcaaggtcacaccgtgttcctggtttcctggcgcaacccggatgcttctatggcaggttctacgtgggatgattacatcgagcacgcggctattcgtgcgatcgaagttgcacgtgatattagcggccaggataagatcaacgtactgggcttttgcgttggcggtactattgttagcactgcactggcagttctggcggcacgtggcgagcatccggctgcatccgttaccctgctgaccaccctgctggattttgcggacactggcatcctggacgtctttgttgatgaaggtcacgtgcagctgcgcgaagcgactctgggtggtggcgcgggtgctccgtgcgcactgctgcgcggcctggaactggctaacactttctcctttctgcgtccgaacgacctggtgtggaactacgttgtggacaactacctgaaaggtaacacgccggtgcctttcgatctgctgttctggaacggcgatgctaccaatctgccgggtccatggtattgctggtacctgcgtcacacttatctgcaaaacgaact
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K338004_sequence
1
atgactcaacgtatcgcatatgtaactggtggtatgggtggtatcggtactgcaatttgccagcgtctggcgaaagacggtttccgtgttgttgcgggctgcggtccgaactccccgcgtcgtgaaaagtggctggaacaacagaaagccctgggcttcgacttcattgcctccgagggtaatgtagctgactgggattccaccaagactgccttcgataaagttaaatctgaagtgggcgaagtagatgtactgatcaacaacgccggtattactcgtgatgtcgtattccgcaaaatgacccgtgcagactgggatgcagttatcgacaccaacctgacgtctctgttcaacgttaccaaacaggttattgatggtatggctgaccgtggctggggccgcatcgtgaacatctctagcgttaacggccaaaaaggccaatttggtcagacgaattacagcacggctaaagcaggcctgcacggtttcaccatggcactggcgcaggaagtggcgaccaaaggtgttaccgttaataccgtttctccaggttacatcgccaccgatatggttaaggctatccgccaagatgttctggacaagatcgtggctaccattccggttaaacgcctgggcctgccggaagaaattgcgtccatctgtgcgtggctgagctccgaagagtctggtttttccaccggtgcggatttctctctgaacggtggtctgcacatgggttgatactagagaaagaggagaaatactagatggcaactggtaaaggtgctgctgcatctactcaagaaggtaaatcccagccgttcaaagtgacccctggtccgttcgatccggcgacctggctggaatggagccgccagtggcagggcactgaaggcaacggccacgcagctgcttctggcattccgggtctggacgctctggcgggcgtaaaaatcgcaccggcacaactgggcgacatccagcaacgttatatgaaagatttcagcgcgctgtggcaggcgatggcggaaggcaaagccgaagcgaccggtccgctgcacgaccgtcgtttcgcaggtgacgcgtggcgtaccaacctgccataccgtttcgctgccgccttttacctgctgaacgcacgtgcgctgaccgaactggctgatgcagttgaagctgacgccaaaacccgccagcgtatccgtttcgctatcagccagtgggtcgacgcgatgagcccggccaacttcctggccacgaacccggaggcccagcgcctgctgatcgaatctggtggtgaatctctgcgtgcgggcgttcgtaacatgatggaggatctgacgcgcggtaagatctctcaaaccgacgagagcgcgtttgaagttggccgtaacgtggcggttactgaaggcgcggttgttttcgagaacgaatatttccagctgctgcaatacaaaccgctgaccgacaaggttcacgctcgtccgctgctgatggtaccgccttgcatcaacaaatactacatcctggacctgcaaccagaatcctctctggttcgtcacgtggttgagcaaggtcacaccgtgttcctggtttcctggcgcaacccggatgcttctatggcaggttctacgtgggatgattacatcgagcacgcggctattcgtgcgatcgaagttgcacgtgatattagcggccaggataagatcaacgtactgggcttttgcgttggcggtactattgttagcactgcactggcagttctggcggcacgtggcgagcatccggctgcatccgttaccctgctgaccaccctgctggattttgcggacactggcatcctggacgtctttgttgatgaaggtcacgtgcagctgcgcgaagcgactctgggtggtggcgcgggtgctccgtgcgcactgctgcgcggcctggaactggctaacactttctcctttctgcgtccgaacgacctggtgtggaactacgttgtggacaactacctgaaaggtaacacgccggtgcctttcgatctgctgttctggaacggcgatgctaccaatctgccgggtccatggtattgctggtacctgcgtcacacttatctgcaaaacgaacttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z