BBa_K338022 1 BBa_K338022 Plastic Intermediate: phaA + RBS 2010-10-26T11:00:00Z 2015-05-08T01:12:07Z The Registry of Standard Biological Parts A construction intermediate used to produce <partinfo>BBa_K338003</partinfo>. Contains the first of three genes required to synthesize polyhydroxybutyrate (PHB). false false _454_ 0 6013 9 It's complicated false Designed to be ligated upstream of <partinfo>BBa_K338023</partinfo> and <partinfo>BBa_K338024</partinfo>. false Lucas Hartsough component2104851 1 BBa_K156012 component2104853 1 BBa_B0034 annotation2104853 1 BBa_B0034 range2104853 1 1191 1202 annotation2104851 1 BBa_K156012 range2104851 1 1 1182 BBa_K156012 1 Bioplastic phaA (acetyl-CoA acetyltransferase) 2008-10-28T12:00:00Z 2015-05-08T01:10:54Z This coding region is native to ''Ralstonia eutropha'' H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031). This part codes for an enzyme that catalyzes the synthesis of acetoacetyl-coenzyme A from two molecules of acetyl-coenzyme A. The enzyme can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation. false false _246_ 0 1475 9 It's complicated false This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI. false George McArthur IV BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K338022_sequence 1 atgactgatgttgttattgtatctgccgctcgtactgctgttggtaaatttggtggtagcctggctaaaatcccggcacctgagctgggcgctgtagttatcaaagctgcgctggaacgtgcgggcgttaaaccggagcaagtgtccgaagtcatcatgggccaagtactgacggcgggcagcggccagaacccggctcgccaggcggccattaaagcaggtctgccggccatggtgccggcaatgaccattaacaaagtttgcggttctggtctgaaagctgttatgctggcagctaatgcaatcatggcaggtgacgcagagattgttgtcgctggtggtcaagaaaacatgagcgctgctccgcatgttctgccaggctcccgtgacggtttccgtatgggtgatgcaaaactggtggacactatgatcgtcgacggcctgtgggatgtgtataaccagtatcacatgggtattaccgcagagaacgttgcgaaagaatatggcattacgcgtgaagcgcaggacgaatttgcagtgggttctcagaacaaagccgaagcggctcagaaagctggtaagttcgacgaagaaatcgtaccggttctgattccgcagcgcaagggtgatccggtagcattcaaaaccgatgagttcgtacgccagggcgctactctggattccatgtctggcctgaaaccggcattcgacaaagcaggtaccgtgacggctgcgaacgcgagcggcctgaacgatggcgcggctgcggttgtggtcatgagcgcggcgaaagctaaagaactgggcctgacgccgctggcgactatcaaatcttacgcgaacgcaggtgtggatccgaaggtgatgggtatgggtcctgtgccagctagcaagcgcgctctgtcccgcgctgaatggactccgcaggatctggatctgatggagattaacgaagctttcgctgcgcaggcgctggcggtacatcagcagatgggttgggacacttctaaagtcaatgtaaacggcggtgccattgcgatcggccacccgatcggcgcttccggttgccgcatcctggtaaccctgctgcacgaaatgaaacgtcgtgacgctaagaaaggtctggcctctctgtgcattggtggcggcatgggtgttgccctggccgttgaacgtaaatgatactagagaaagaggagaaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K156012_sequence 1 atgactgatgttgttattgtatctgccgctcgtactgctgttggtaaatttggtggtagcctggctaaaatcccggcacctgagctgggcgctgtagttatcaaagctgcgctggaacgtgcgggcgttaaaccggagcaagtgtccgaagtcatcatgggccaagtactgacggcgggcagcggccagaacccggctcgccaggcggccattaaagcaggtctgccggccatggtgccggcaatgaccattaacaaagtttgcggttctggtctgaaagctgttatgctggcagctaatgcaatcatggcaggtgacgcagagattgttgtcgctggtggtcaagaaaacatgagcgctgctccgcatgttctgccaggctcccgtgacggtttccgtatgggtgatgcaaaactggtggacactatgatcgtcgacggcctgtgggatgtgtataaccagtatcacatgggtattaccgcagagaacgttgcgaaagaatatggcattacgcgtgaagcgcaggacgaatttgcagtgggttctcagaacaaagccgaagcggctcagaaagctggtaagttcgacgaagaaatcgtaccggttctgattccgcagcgcaagggtgatccggtagcattcaaaaccgatgagttcgtacgccagggcgctactctggattccatgtctggcctgaaaccggcattcgacaaagcaggtaccgtgacggctgcgaacgcgagcggcctgaacgatggcgcggctgcggttgtggtcatgagcgcggcgaaagctaaagaactgggcctgacgccgctggcgactatcaaatcttacgcgaacgcaggtgtggatccgaaggtgatgggtatgggtcctgtgccagctagcaagcgcgctctgtcccgcgctgaatggactccgcaggatctggatctgatggagattaacgaagctttcgctgcgcaggcgctggcggtacatcagcagatgggttgggacacttctaaagtcaatgtaaacggcggtgccattgcgatcggccacccgatcggcgcttccggttgccgcatcctggtaaccctgctgcacgaaatgaaacgtcgtgacgctaagaaaggtctggcctctctgtgcattggtggcggcatgggtgttgccctggccgttgaacgtaaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z