BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K338024 1 BBa_K338024 Plastic Intermediate: phaC1 + Terminator 2010-10-26T11:00:00Z 2015-05-08T01:12:07Z The Registry of Standard Biological Parts A construction intermediate used to produce <partinfo>BBa_K338004</partinfo>. Contains the third of three genes required to synthesize polyhydroxybutyrate (PHB). false false _454_ 0 6013 9 It's complicated false Designed to be ligated downstream of <partinfo>K338022</partinfo> and <partinfo>K338023</partinfo>. false Lucas Hartsough component2104861 1 BBa_B0015 component2104854 1 BBa_K156014 annotation2104854 1 BBa_K156014 range2104854 1 1 1349 annotation2104861 1 BBa_B0015 range2104861 1 1358 1486 BBa_K156014 1 Bioplastic phaC1 (Poly(3-hydroxybutyrate) polymerase) 2008-10-28T12:00:00Z 2015-05-08T01:10:54Z This coding region is native to Ralstonia eutropha H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031). This part codes for a polyhydroxybutyrate (PHB) synthase, which catalyzes the polymerization of hydroxybutyryl-CoA. false false _246_ 0 1475 9 It's complicated false This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI. false George McArthur IV BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K156014_sequence 1 atggcaactggtaaaggtgctgctgcatctactcaagaaggtaaatcccagccgttcaaagtgacccctggtccgttcgatccggcgacctggctggaatggagccgccagtggcagggcactgaaggcaacggccacgcagctgcttctggcattccgggtctggacgctctggcgggcgtaaaaatcgcaccggcacaactgggcgacatccagcaacgttatatgaaagatttcagcgcgctgtggcaggcgatggcggaaggcaaagccgaagcgaccggtccgctgcacgaccgtcgtttcgcaggtgacgcgtggcgtaccaacctgccataccgtttcgctgccgccttttacctgctgaacgcacgtgcgctgaccgaactggctgatgcagttgaagctgacgccaaaacccgccagcgtatccgtttcgctatcagccagtgggtcgacgcgatgagcccggccaacttcctggccacgaacccggaggcccagcgcctgctgatcgaatctggtggtgaatctctgcgtgcgggcgttcgtaacatgatggaggatctgacgcgcggtaagatctctcaaaccgacgagagcgcgtttgaagttggccgtaacgtggcggttactgaaggcgcggttgttttcgagaacgaatatttccagctgctgcaatacaaaccgctgaccgacaaggttcacgctcgtccgctgctgatggtaccgccttgcatcaacaaatactacatcctggacctgcaaccagaatcctctctggttcgtcacgtggttgagcaaggtcacaccgtgttcctggtttcctggcgcaacccggatgcttctatggcaggttctacgtgggatgattacatcgagcacgcggctattcgtgcgatcgaagttgcacgtgatattagcggccaggataagatcaacgtactgggcttttgcgttggcggtactattgttagcactgcactggcagttctggcggcacgtggcgagcatccggctgcatccgttaccctgctgaccaccctgctggattttgcggacactggcatcctggacgtctttgttgatgaaggtcacgtgcagctgcgcgaagcgactctgggtggtggcgcgggtgctccgtgcgcactgctgcgcggcctggaactggctaacactttctcctttctgcgtccgaacgacctggtgtggaactacgttgtggacaactacctgaaaggtaacacgccggtgcctttcgatctgctgttctggaacggcgatgctaccaatctgccgggtccatggtattgctggtacctgcgtcacacttatctgcaaaacgaact BBa_K338024_sequence 1 atggcaactggtaaaggtgctgctgcatctactcaagaaggtaaatcccagccgttcaaagtgacccctggtccgttcgatccggcgacctggctggaatggagccgccagtggcagggcactgaaggcaacggccacgcagctgcttctggcattccgggtctggacgctctggcgggcgtaaaaatcgcaccggcacaactgggcgacatccagcaacgttatatgaaagatttcagcgcgctgtggcaggcgatggcggaaggcaaagccgaagcgaccggtccgctgcacgaccgtcgtttcgcaggtgacgcgtggcgtaccaacctgccataccgtttcgctgccgccttttacctgctgaacgcacgtgcgctgaccgaactggctgatgcagttgaagctgacgccaaaacccgccagcgtatccgtttcgctatcagccagtgggtcgacgcgatgagcccggccaacttcctggccacgaacccggaggcccagcgcctgctgatcgaatctggtggtgaatctctgcgtgcgggcgttcgtaacatgatggaggatctgacgcgcggtaagatctctcaaaccgacgagagcgcgtttgaagttggccgtaacgtggcggttactgaaggcgcggttgttttcgagaacgaatatttccagctgctgcaatacaaaccgctgaccgacaaggttcacgctcgtccgctgctgatggtaccgccttgcatcaacaaatactacatcctggacctgcaaccagaatcctctctggttcgtcacgtggttgagcaaggtcacaccgtgttcctggtttcctggcgcaacccggatgcttctatggcaggttctacgtgggatgattacatcgagcacgcggctattcgtgcgatcgaagttgcacgtgatattagcggccaggataagatcaacgtactgggcttttgcgttggcggtactattgttagcactgcactggcagttctggcggcacgtggcgagcatccggctgcatccgttaccctgctgaccaccctgctggattttgcggacactggcatcctggacgtctttgttgatgaaggtcacgtgcagctgcgcgaagcgactctgggtggtggcgcgggtgctccgtgcgcactgctgcgcggcctggaactggctaacactttctcctttctgcgtccgaacgacctggtgtggaactacgttgtggacaactacctgaaaggtaacacgccggtgcctttcgatctgctgttctggaacggcgatgctaccaatctgccgggtccatggtattgctggtacctgcgtcacacttatctgcaaaacgaacttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z