BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K338001
1
HSP
Heat Shock Promoter (HSP)
2010-10-23T11:00:00Z
2015-05-08T01:12:07Z
Modified BBa_K112400
This is a modified K112400 heat shock promoter in BBa standard.
false
false
_454_
0
6189
9
It's complicated
true
None
false
2010 Caltech iGEM Team
BBa_K338081
1
BBa_K338081
Heat-Shock Activated Generator
2010-10-26T11:00:00Z
2015-05-08T01:12:07Z
The Registry of Standard Biological Parts
Intermediate designed to accelerate cloning of the HSP promoter (<partinfo>BBa_K338001</partinfo>) by adding an RBS downstream.
false
false
_454_
0
6013
9
It's complicated
false
Designed to be ligated upstream of the desired heat-shock product. Keep in mind that the promoter is somewhat leaky and that cultures should be grown at 30°C.
false
Lucas Hartsough
component2105680
1
BBa_B0034
component2105678
1
BBa_K338001
annotation2105680
1
BBa_B0034
range2105680
1
107
118
annotation2105678
1
BBa_K338001
range2105678
1
1
98
BBa_K338081_sequence
1
ccgaggtccttgttgcgaagattgatgacaatgtgagtgcttcccttgaaaccctgaaactgatccccataataagcgaagttagcgagatgaatgcgtactagagaaagaggagaaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K338001_sequence
1
ccgaggtccttgttgcgaagattgatgacaatgtgagtgcttcccttgaaaccctgaaactgatccccataataagcgaagttagcgagatgaatgcg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z