LOCUS       BBa_K339009             3359 bp    DNA     linear       29-Nov-2024
DEFINITION  CpxR Promoter with RFP Reporter and Arabinose Inducible MalE31
ACCESSION   BBa_K339009
VERSION     BBa_K339009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..15
                     /label=CpxR-P Binding site 
     promoter        1..55
                     /label=BBa_K135000
     promoter        1..55
                     /label=BBa_K135000
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..756
                     /label=mrfp1
     misc_feature    763..787
                     /label=Help:Barcodes
     CDS             82..762
                     /label=BBa_E1010
     stem_loop       807..850
                     /label=stem_loop
     misc_feature    796..875
                     /label=BBa_B0010
     terminator      796..875
                     /label=BBa_B0010
     stem_loop       891..910
                     /label=T7 TE
     misc_feature    917..917
                     /label=stop
     polyA_site      911..924
                     /label=polya
     misc_feature    884..924
                     /label=BBa_B0012
     terminator      884..924
                     /label=BBa_B0012
     misc_feature    64..924
                     /label=BBa_I13507
     promoter        2012..2142
                     /label=Pbad
     promoter        1975..2046
                     /label=PC
     misc_feature    933..2011
                     /label=AraC
     promoter        933..2142
                     /label=BBa_I0500
     misc_feature    2155..2158
                     /label=conserved
     RBS             2151..2162
                     /label=BBa_B0034
     misc_feature    2169..2171
                     /label=START
     misc_feature    3357..3359
                     /label=STOP
     misc_feature    2339..2345
                     /label=malE31 Mutation
     misc_feature    2172..2247
                     /label=Signal Sequence
     CDS             2169..3359
                     /label=BBa_K339001
ORIGIN
        1 gtaaaacaac gtaaagtcat ggattagcga cgtctgatga cgtaatttct gcctctacta
       61 gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt
      121 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt
      181 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg
      241 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt
      301 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg
      361 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg
      421 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt
      481 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa
      541 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac
      601 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac
      661 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag
      721 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt
      781 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      841 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      901 cgggtgggcc tttctgcgtt tatatactag agttatgaca acttgacggc tacatcattc
      961 actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat
     1021 acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc
     1081 atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt
     1141 aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa
     1201 acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac
     1261 tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc
     1321 atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct
     1381 tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct
     1441 tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc
     1501 cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga
     1561 cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca
     1621 aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa
     1681 cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc
     1741 gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc
     1801 gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc
     1861 atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc
     1921 ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac
     1981 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
     2041 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg
     2101 caactctcta ctgtttctcc atacccgttt ttttgggcta gctactagag aaagaggaga
     2161 aatactagat gaaaataaaa acaggtgcac gcatcctcgc attatccgca ttaacgacga
     2221 tgatgttttc cgcctcggct ctcgccaagc ttgaagaagg taaactggta atctggatta
     2281 acggcgataa aggctataac ggtctcgctg aagtcggtaa gaaattcgag aaagatacgg
     2341 atccgaaagt caccgttgag catccggata aactggaaga gaaattccca caggttgcgg
     2401 caactggcga tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc
     2461 aatctggcct gttggctgaa atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt
     2521 ttacctggga tgccgtacgt tacaacggca agctgattgc ttacccgatc gctgttgaag
     2581 cgttatcgct gatttataac aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga
     2641 tcccggcgct ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc
     2701 aagaaccgta cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg
     2761 aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc
     2821 tgaccttcct ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca
     2881 tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat
     2941 ggtccaacat cgacaccagc aaagtgaatt atggtgtaac ggtactgccg accttcaagg
     3001 gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc gccagtccga
     3061 acaaagagct ggcgaaagag ttcctcgaaa actatctgct gactgatgaa ggtctggaag
     3121 cggttaataa agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg
     3181 cgaaagatcc acgtattgcc gccaccatgg aaaacgccca gaaaggtgaa atcatgccga
     3241 acatcccgca gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca
     3301 gcggtcgtca gactgtcgat gaagccctga aagacgcgca gactcgtatc accaagtaa
//