##gff-version 3
##sequence-region BBa_K339009 1 3359
BBa_K339009	.	promoter	1	55	.	+	0	ID=BBa_K135000;Name=BBa_K135000
BBa_K339009	.	non_covalent_binding_site	1	15	.	+	0	ID=annotation1982751;Name=CpxR-P Binding site ;Parent=BBa_K135000
BBa_K339009	.	promoter	1	55	.	+	0	ID=annotation1982752;Name=BBa_K135000;Parent=BBa_K135000
BBa_K339009	.	promoter	933	2142	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_K339009	.	promoter	1975	2046	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_K339009	.	promoter	2012	2142	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_K339009	.	sequence_feature	933	2011	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_K339009	.	ribosome_entry_site	2151	2162	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K339009	.	conserved_region	2155	2158	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K339009	.	engineered_region	64	924	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K339009	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K339009	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K339009	.	terminator	884	924	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K339009	.	polyA_site	911	924	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K339009	.	engineered_region	884	924	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K339009	.	stem_loop	891	910	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K339009	.	stop_codon	917	917	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K339009	.	terminator	796	875	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K339009	.	stem_loop	807	850	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K339009	.	engineered_region	796	875	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K339009	.	CDS	82	762	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K339009	.	CDS	82	756	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K339009	.	engineered_tag	763	787	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K339009	.	CDS	2169	3359	.	+	0	ID=BBa_K339001;Name=BBa_K339001
BBa_K339009	.	start_codon	2169	2171	.	+	0	ID=annotation2093639;Name=START;Parent=BBa_K339001
BBa_K339009	.	sequence_alteration	2339	2345	.	+	0	ID=annotation2113544;Name=malE31 Mutation;Parent=BBa_K339001
BBa_K339009	.	stop_codon	3357	3359	.	+	0	ID=annotation2113543;Name=STOP;Parent=BBa_K339001
BBa_K339009	.	tag	2172	2247	.	+	0	ID=annotation2113638;Name=Signal Sequence;Parent=BBa_K339001
>BBa_K339009
gtaaaacaacgtaaagtcatggattagcgacgtctgatgacgtaatttctgcctctactagagaaagaggagaaatact
agatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacga
gttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggt
ccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgaca
tcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgt
taccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtcc
gacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctga
aaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaa
aaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatc
gttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacg
gctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcg
agaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagctt
cgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgc
gacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgac
aagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctc
aagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctg
aaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgcc
agtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctc
tcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcga
atggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcg
gcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactttt
catactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctc
ttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgc
gtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagc
atttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttt
tgggctagctactagagaaagaggagaaatactagatgaaaataaaaacaggtgcacgcatcctcgcattatccgcatt
aacgacgatgatgttttccgcctcggctctcgccaagcttgaagaaggtaaactggtaatctggattaacggcgataaa
ggctataacggtctcgctgaagtcggtaagaaattcgagaaagatacggatccgaaagtcaccgttgagcatccggata
aactggaagagaaattcccacaggttgcggcaactggcgatggccctgacattatcttctgggcacacgaccgctttgg
tggctacgctcaatctggcctgttggctgaaatcaccccggacaaagcgttccaggacaagctgtatccgtttacctgg
gatgccgtacgttacaacggcaagctgattgcttacccgatcgctgttgaagcgttatcgctgatttataacaaagatc
tgctgccgaacccgccaaaaacctgggaagagatcccggcgctggataaagaactgaaagcgaaaggtaagagcgcgct
gatgttcaacctgcaagaaccgtacttcacctggccgctgattgctgctgacgggggttatgcgttcaagtatgaaaac
ggcaagtacgacattaaagacgtgggcgtggataacgctggcgcgaaagcgggtctgaccttcctggttgacctgatta
aaaacaaacacatgaatgcagacaccgattactccatcgcagaagctgcctttaataaaggcgaaacagcgatgaccat
caacggcccgtgggcatggtccaacatcgacaccagcaaagtgaattatggtgtaacggtactgccgaccttcaagggt
caaccatccaaaccgttcgttggcgtgctgagcgcaggtattaacgccgccagtccgaacaaagagctggcgaaagagt
tcctcgaaaactatctgctgactgatgaaggtctggaagcggttaataaagacaaaccgctgggtgccgtagcgctgaa
gtcttacgaggaagagttggcgaaagatccacgtattgccgccaccatggaaaacgcccagaaaggtgaaatcatgccg
aacatcccgcagatgtccgctttctggtatgccgtgcgtactgcggtgatcaacgccgccagcggtcgtcagactgtcg
atgaagccctgaaagacgcgcagactcgtatcaccaagtaa