BBa_K341000 1 BBa_K341000 recombination sequence + eGFP+I-Sce I cutting site 2010-10-17T11:00:00Z 2015-05-08T01:12:07Z From plasmid containing gene eGFP. The backbone of our part BBa-K341000_eGFP_recombination is PSB1C13, resistant to Amp. Restriction enzyme I-SceI cutting site, Recombination sequence 1, homologous to part of modified E.coli genome, coding sequence of gene eGFP, recombination sequence 2, homologous to another part of modified E.coli genome, I-Scel cutting site( the elements detailed above are listed in the order from 5??? to 3???) are added into the backbone with EcoR I and Pst I. The biobrick we construct in this part is to verify landing pad method, on which our project is based on. false false _468_ 0 6053 9 It's complicated false false Danyang He annotation2090505 1 eGFP range2090505 1 43 759 annotation2090506 1 recombination sequence 1 range2090506 1 43 67 annotation2090507 1 recombination sequence 2 range2090507 1 739 759 annotation2090504 1 I-Sce I cutting site range2090504 1 11 26 BBa_K341000_sequence 1 cagcgtcgactagggataacagggtaataggaggtcactgtaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaaaagcttgtgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z