BBa_K341456
1
BBa_K341456
Kanamycin resistance(with Promoter) + I-sceI site+ Recombination site
2010-10-20T11:00:00Z
2015-05-08T01:12:08Z
Kanamycin resistance gene is form plasmid "pKD13"
Kanamycin resistance(with Promoter) + I-sceI site+ Recombination site+DraIII cutting site.
This part can be used as a recombination unit between a plasmid and the chromosome in the "In-vivo Recombination System" in iGEM project "Tsinghua 2010"
false
false
_468_
0
4058
9
It's complicated
true
This part involves DraIII cutting site at two ends of the unit, this cutting site is compatible with BBF RFC 61
false
Teng Li
annotation2091141
1
promoter
range2091141
1
863
1229
annotation2091137
1
Dra III cutting site
range2091137
1
1273
1281
annotation2091135
1
Recombination site
range2091135
1
33
57
annotation2091139
1
I-sceI cutting site
range2091139
1
15
32
annotation2091136
1
Recombination site
range2091136
1
1230
1254
annotation2091138
1
Dra III cutting site
range2091138
1
6
14
annotation2091134
1
Km Resistance gene
range2091134
1
68
862
annotation2091140
1
I-sceI cutting site
range2091140
1
1255
1272
BBa_K341456_sequence
1
attatcacggagtgtagggataacagggtaatgatggcgcctcatccctgaagccaagatccccttattagaagaactcgtcaagaaggcgatagaaggcgatgcgctgcgaatcgggagcggcgataccgtaaagcacgaggaagcggtcagcccattcgccgccaagctcttcagcaatatcacgggtagccaacgctatgtcctgatagcggtccgccacacccagccggccacagtcgatgaatccagaaaagcggccattttccaccatgatattcggcaagcaggcatcgccatgggtcacgacgagatcctcgccgtcgggcatgcgcgccttgagcctggcgaacagttcggctggcgcgagcccctgatgctcttcgtccagatcatcctgatcgacaagaccggcttccatccgagtacgtgctcgctcgatgcgatgtttcgcttggtggtcgaatgggcaggtagccggatcaagcgtatgcagccgccgcattgcatcagccatgatggatactttctcggcaggagcaaggtgagatgacaggagatcctgccccggcacttcgcccaatagcagccagtcccttcccgcttcagtgacaacgtcgagcacagctgcgcaaggaacgcccgtcgtggccagccacgatagccgcgctgcctcgtcctgtagttcattcagggcaccggacaggtcggtcttgacaaaaagaaccgggcgcccctgcgctgacagccggaacacggcggcatcagagcagccgattgtctgttgtgcccagtcatagccgaatagcctctccacccaagcggccggagaacctgcgtgcaatccatcttgttcaatcatgcgaaacgatcctcatcctgtctcttgatcagatcttgatcccctgcgccatcagatccttggcggcaagaaagccatccagtttactttgcagggcttcccaaccttaccagagggcgccccagctggcaattccggttcgcttgctgtccataaaaccgcccagtctagctatcgccatgtaagcccactgcaagctacctgctttctctttgcgcttgcgttttcccttgtccagatagcccagtagctgacattcatccggggtcagcaccgtttctgcggactggctttctacgtgttccgcttcctttagcagcccttgcgccctgagtgcttgcggcagcgtgagcttcaaaagcgctcttcaccgtttggaccttggggccgtaattaccctgttatccctacaccaggtgataat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z