##gff-version 3 ##sequence-region BBa_K343005 1 1943 BBa_K343005 . CDS 81 1943 . + 0 ID=BBa_K343001;Name=BBa_K343001 BBa_K343005 . start_codon 81 83 . + 0 ID=annotation2072320;Name=met;Parent=BBa_K343001 BBa_K343005 . stop_codon 1941 1943 . + 0 ID=annotation2072321;Name=stop;Parent=BBa_K343001 BBa_K343005 . CDS 81 1940 . + 0 ID=annotation2072319;Name=Coding sequence;Parent=BBa_K343001 BBa_K343005 . ribosome_entry_site 63 74 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_K343005 . conserved_region 67 70 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K343005 . promoter 1 54 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_K343005 . promoter 20 25 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_K343005 . promoter 43 48 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_K343005 . non_covalent_binding_site 1 19 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_K343005 . engineered_region 1 54 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_K343005 . non_covalent_binding_site 26 44 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 >BBa_K343005 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta gatggcagccggtgtcttcaagagttttatgcgcgacttctttgcggtgaaatacgatgagcagcgaaatgatccgcaa gcggaacgactggatggcaacggacgactgtatcccaactgctcgtcggatgtgtggctgcgatcctgcgagcgggaga tagttgatcccattgagggccatcacagcgggcacattcccaaatggatatgcggtagtctgttgcgcaatggacccgg cagctggaaggtgggcgacatgaccttcggccatctgttcgactgctccgccctgctgcaccgatttgccattcggaat ggacgcgtcacctaccagaatcgcttcgtggacacggaaacactgcgaaagaatcgctctgcccagcggattgtggtca cggagtttggcacagctgctgttccggatccctgtcactcgatcttcgatagatttgcggccatttttcgaccggatag tggaacggataactcgatgatttccatatatcctttcggggatcagtattacacatttacggagacgccttttatgcat agaataaatccctgcactttggccaccgaagcacgaatctgcaccaccgacttcgtgggcgtggtgaaccacacatcgc atccgcatgttcttcccagtggcactgtctacaacctgggcaccacaatgaccagatctggaccggcatacactatact cagtttcccgcacggcgagcagatgttcgaggatgctcatgtggtggccacactgccgtgccgctggaaactgcatccc ggttatatgcacaccttcggcttaacggatcactactttgtgattgtggagcagccgttgtccgtttcgcttacggagt atatcaaagcccagctaggtggacagaatttatcggcgtgtctcaagtggttcgaggatcgaccgacactatttcacct tatagatcgggtttccggcaaactggtgcagacctacgaatcggaagccttcttctacctgcacatcatcaactgcttt gaacgggatggccacgtggtggtggacatttgcagctacaggaatcccgagatgatcaactgcatgtatctggaggcca ttgccaatatgcaaacgaatcccaattatgctaccctctttcgtggacgtcccttgagattcgtcctgcccttgggcac aattcctccggcaagcatcgccaagcggggactggtcaagtccttctcccttgctggactaagtgctccgcaggtttct cgcaccatgaagcactcggtctcgcaatatgcggatataacctacatgcccaccaatggaaagcaagccactgctggag aggaaagccccaagcgagatgccaaacgtggccgctatgaggaggagaatcttgtcaatctggttaccatggagggcag tcaagcggaggcgtttcagggcaccaatggcatccaactgcgtccggaaatgctgtgtgattggggctgtgaaacacct aggatctattatgaacggtatatgggcaagaactaccgatacttctacgcgattagctccgatgtggatgcagtgaatc cgggcaccctcatcaaggtggatgtgtggaataagagctgtctaacctggtgcgaggagaatgtctatcccagtgagcc catttttgtgccttcgccggatccgaaatccgaggacgatggcgttatcctggcctccatggtgctgggcggtctcaac gatcgctatgtgggcctaattgtgctatgtgccaaaacgatgaccgagctgggccgttgtgatttccataccaatggac ccgtgcccaagtgtctccatggatggtttgcacccaatgccatttag