BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K343001 1 ninaB B-carotene monooxygenase, Produces retinal from B-carotene. ninaB gene from Drosophila. 2010-07-01T11:00:00Z 2015-05-08T01:12:08Z Sequence taken from cDNA This coding region encodes a beta-carotene deoxygenase, that splits beta-carotene into two retinal molecules. false false _465_ 0 6083 9 It's complicated true No considerations have been made so far concerning design false Christian Kurtzhals annotation2072319 1 Coding sequence range2072319 1 1 1860 annotation2072321 1 stop range2072321 1 1861 1863 annotation2072320 1 met range2072320 1 1 3 BBa_K343005 1 ninabGen Beta-carotene 15 2010-09-06T11:00:00Z 2015-05-08T01:12:08Z [link:https://dgrc.cgb.indiana.edu/product/View?product=4630 Drosophila genomics ressource centre], Indiana University. Found via [link:http://www.flybase.org flybase], ID: [link:http://flybase.org/reports/FBcl0129473.html FBcl0129473] The Beta-carotene 15'15'-monooxygenase generator from the D. melanogaster ninaB gene, cleaves beta-carotene into two retinal molecules. Used together with BBa_274210 (beta-carotene generator) it should induce retinal biosynthesis in e. coli. false false _465_ 0 6083 9 It's complicated true A constitutive sigma-70 promotor was chosen. Terminator is lacking on this part. false Christian Kurtzhals component2079706 1 BBa_B0034 component2079700 1 BBa_R0040 component2079710 1 BBa_K343001 annotation2079700 1 BBa_R0040 range2079700 1 1 54 annotation2079710 1 BBa_K343001 range2079710 1 81 1943 annotation2079706 1 BBa_B0034 range2079706 1 63 74 BBa_K343005_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcagccggtgtcttcaagagttttatgcgcgacttctttgcggtgaaatacgatgagcagcgaaatgatccgcaagcggaacgactggatggcaacggacgactgtatcccaactgctcgtcggatgtgtggctgcgatcctgcgagcgggagatagttgatcccattgagggccatcacagcgggcacattcccaaatggatatgcggtagtctgttgcgcaatggacccggcagctggaaggtgggcgacatgaccttcggccatctgttcgactgctccgccctgctgcaccgatttgccattcggaatggacgcgtcacctaccagaatcgcttcgtggacacggaaacactgcgaaagaatcgctctgcccagcggattgtggtcacggagtttggcacagctgctgttccggatccctgtcactcgatcttcgatagatttgcggccatttttcgaccggatagtggaacggataactcgatgatttccatatatcctttcggggatcagtattacacatttacggagacgccttttatgcatagaataaatccctgcactttggccaccgaagcacgaatctgcaccaccgacttcgtgggcgtggtgaaccacacatcgcatccgcatgttcttcccagtggcactgtctacaacctgggcaccacaatgaccagatctggaccggcatacactatactcagtttcccgcacggcgagcagatgttcgaggatgctcatgtggtggccacactgccgtgccgctggaaactgcatcccggttatatgcacaccttcggcttaacggatcactactttgtgattgtggagcagccgttgtccgtttcgcttacggagtatatcaaagcccagctaggtggacagaatttatcggcgtgtctcaagtggttcgaggatcgaccgacactatttcaccttatagatcgggtttccggcaaactggtgcagacctacgaatcggaagccttcttctacctgcacatcatcaactgctttgaacgggatggccacgtggtggtggacatttgcagctacaggaatcccgagatgatcaactgcatgtatctggaggccattgccaatatgcaaacgaatcccaattatgctaccctctttcgtggacgtcccttgagattcgtcctgcccttgggcacaattcctccggcaagcatcgccaagcggggactggtcaagtccttctcccttgctggactaagtgctccgcaggtttctcgcaccatgaagcactcggtctcgcaatatgcggatataacctacatgcccaccaatggaaagcaagccactgctggagaggaaagccccaagcgagatgccaaacgtggccgctatgaggaggagaatcttgtcaatctggttaccatggagggcagtcaagcggaggcgtttcagggcaccaatggcatccaactgcgtccggaaatgctgtgtgattggggctgtgaaacacctaggatctattatgaacggtatatgggcaagaactaccgatacttctacgcgattagctccgatgtggatgcagtgaatccgggcaccctcatcaaggtggatgtgtggaataagagctgtctaacctggtgcgaggagaatgtctatcccagtgagcccatttttgtgccttcgccggatccgaaatccgaggacgatggcgttatcctggcctccatggtgctgggcggtctcaacgatcgctatgtgggcctaattgtgctatgtgccaaaacgatgaccgagctgggccgttgtgatttccataccaatggacccgtgcccaagtgtctccatggatggtttgcacccaatgccatttag BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K343001_sequence 1 atggcagccggtgtcttcaagagttttatgcgcgacttctttgcggtgaaatacgatgagcagcgaaatgatccgcaagcggaacgactggatggcaacggacgactgtatcccaactgctcgtcggatgtgtggctgcgatcctgcgagcgggagatagttgatcccattgagggccatcacagcgggcacattcccaaatggatatgcggtagtctgttgcgcaatggacccggcagctggaaggtgggcgacatgaccttcggccatctgttcgactgctccgccctgctgcaccgatttgccattcggaatggacgcgtcacctaccagaatcgcttcgtggacacggaaacactgcgaaagaatcgctctgcccagcggattgtggtcacggagtttggcacagctgctgttccggatccctgtcactcgatcttcgatagatttgcggccatttttcgaccggatagtggaacggataactcgatgatttccatatatcctttcggggatcagtattacacatttacggagacgccttttatgcatagaataaatccctgcactttggccaccgaagcacgaatctgcaccaccgacttcgtgggcgtggtgaaccacacatcgcatccgcatgttcttcccagtggcactgtctacaacctgggcaccacaatgaccagatctggaccggcatacactatactcagtttcccgcacggcgagcagatgttcgaggatgctcatgtggtggccacactgccgtgccgctggaaactgcatcccggttatatgcacaccttcggcttaacggatcactactttgtgattgtggagcagccgttgtccgtttcgcttacggagtatatcaaagcccagctaggtggacagaatttatcggcgtgtctcaagtggttcgaggatcgaccgacactatttcaccttatagatcgggtttccggcaaactggtgcagacctacgaatcggaagccttcttctacctgcacatcatcaactgctttgaacgggatggccacgtggtggtggacatttgcagctacaggaatcccgagatgatcaactgcatgtatctggaggccattgccaatatgcaaacgaatcccaattatgctaccctctttcgtggacgtcccttgagattcgtcctgcccttgggcacaattcctccggcaagcatcgccaagcggggactggtcaagtccttctcccttgctggactaagtgctccgcaggtttctcgcaccatgaagcactcggtctcgcaatatgcggatataacctacatgcccaccaatggaaagcaagccactgctggagaggaaagccccaagcgagatgccaaacgtggccgctatgaggaggagaatcttgtcaatctggttaccatggagggcagtcaagcggaggcgtttcagggcaccaatggcatccaactgcgtccggaaatgctgtgtgattggggctgtgaaacacctaggatctattatgaacggtatatgggcaagaactaccgatacttctacgcgattagctccgatgtggatgcagtgaatccgggcaccctcatcaaggtggatgtgtggaataagagctgtctaacctggtgcgaggagaatgtctatcccagtgagcccatttttgtgccttcgccggatccgaaatccgaggacgatggcgttatcctggcctccatggtgctgggcggtctcaacgatcgctatgtgggcctaattgtgctatgtgccaaaacgatgaccgagctgggccgttgtgatttccataccaatggacccgtgcccaagtgtctccatggatggtttgcacccaatgccatttag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z