LOCUS       BBa_K343006             2080 bp    DNA     linear       25-Nov-2024
DEFINITION  Expresses B-carotene monooxygenase on a constitutive promotor
ACCESSION   BBa_K343006
VERSION     BBa_K343006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1940
                     /label=Coding sequence
     misc_feature    81..83
                     /label=met
     misc_feature    1941..1943
                     /label=stop
     CDS             81..1943
                     /label=BBa_K343001
     stem_loop       1963..2006
                     /label=stem_loop
     misc_feature    1952..2031
                     /label=BBa_B0010
     terminator      1952..2031
                     /label=BBa_B0010
     stem_loop       2047..2066
                     /label=T7 TE
     misc_feature    2073..2073
                     /label=stop
     polyA_site      2067..2080
                     /label=polya
     misc_feature    2040..2080
                     /label=BBa_B0012
     terminator      2040..2080
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcagccg gtgtcttcaa gagttttatg cgcgacttct
      121 ttgcggtgaa atacgatgag cagcgaaatg atccgcaagc ggaacgactg gatggcaacg
      181 gacgactgta tcccaactgc tcgtcggatg tgtggctgcg atcctgcgag cgggagatag
      241 ttgatcccat tgagggccat cacagcgggc acattcccaa atggatatgc ggtagtctgt
      301 tgcgcaatgg acccggcagc tggaaggtgg gcgacatgac cttcggccat ctgttcgact
      361 gctccgccct gctgcaccga tttgccattc ggaatggacg cgtcacctac cagaatcgct
      421 tcgtggacac ggaaacactg cgaaagaatc gctctgccca gcggattgtg gtcacggagt
      481 ttggcacagc tgctgttccg gatccctgtc actcgatctt cgatagattt gcggccattt
      541 ttcgaccgga tagtggaacg gataactcga tgatttccat atatcctttc ggggatcagt
      601 attacacatt tacggagacg ccttttatgc atagaataaa tccctgcact ttggccaccg
      661 aagcacgaat ctgcaccacc gacttcgtgg gcgtggtgaa ccacacatcg catccgcatg
      721 ttcttcccag tggcactgtc tacaacctgg gcaccacaat gaccagatct ggaccggcat
      781 acactatact cagtttcccg cacggcgagc agatgttcga ggatgctcat gtggtggcca
      841 cactgccgtg ccgctggaaa ctgcatcccg gttatatgca caccttcggc ttaacggatc
      901 actactttgt gattgtggag cagccgttgt ccgtttcgct tacggagtat atcaaagccc
      961 agctaggtgg acagaattta tcggcgtgtc tcaagtggtt cgaggatcga ccgacactat
     1021 ttcaccttat agatcgggtt tccggcaaac tggtgcagac ctacgaatcg gaagccttct
     1081 tctacctgca catcatcaac tgctttgaac gggatggcca cgtggtggtg gacatttgca
     1141 gctacaggaa tcccgagatg atcaactgca tgtatctgga ggccattgcc aatatgcaaa
     1201 cgaatcccaa ttatgctacc ctctttcgtg gacgtccctt gagattcgtc ctgcccttgg
     1261 gcacaattcc tccggcaagc atcgccaagc ggggactggt caagtccttc tcccttgctg
     1321 gactaagtgc tccgcaggtt tctcgcacca tgaagcactc ggtctcgcaa tatgcggata
     1381 taacctacat gcccaccaat ggaaagcaag ccactgctgg agaggaaagc cccaagcgag
     1441 atgccaaacg tggccgctat gaggaggaga atcttgtcaa tctggttacc atggagggca
     1501 gtcaagcgga ggcgtttcag ggcaccaatg gcatccaact gcgtccggaa atgctgtgtg
     1561 attggggctg tgaaacacct aggatctatt atgaacggta tatgggcaag aactaccgat
     1621 acttctacgc gattagctcc gatgtggatg cagtgaatcc gggcaccctc atcaaggtgg
     1681 atgtgtggaa taagagctgt ctaacctggt gcgaggagaa tgtctatccc agtgagccca
     1741 tttttgtgcc ttcgccggat ccgaaatccg aggacgatgg cgttatcctg gcctccatgg
     1801 tgctgggcgg tctcaacgat cgctatgtgg gcctaattgt gctatgtgcc aaaacgatga
     1861 ccgagctggg ccgttgtgat ttccatacca atggacccgt gcccaagtgt ctccatggat
     1921 ggtttgcacc caatgccatt tagtactaga gccaggcatc aaataaaacg aaaggctcag
     1981 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2041 cacactggct caccttcggg tgggcctttc tgcgtttata
//