BBa_K343006 1 B-carotene Expresses B-carotene monooxygenase on a constitutive promotor 2010-10-13T11:00:00Z 2015-05-08T01:12:08Z The ninaB gene was originally cloned from drosophila melanogaster, and cDNA was aquired for production of the biobrick from the Drosophila Genome Ressource Center. Promotor+rbs brick BBa_J13002 and terminator BBa_B0015 were used. This generator expresses a beta-carotene 15,15'-monooxygenase,on a constitutive promotor, that catalyses retinal production from beta-carotene via the following reaction: beta-carotene + O2 = 2 retinal false false _465_ 0 6083 9 It's complicated true The brick requires B-carotene supply to function. This can either be supplied externally, or alternatively the BBa_K274210 B-carotene generator will induce full B-carotene synthesis in E. coli. false Christian Kurtzhals component2085991 1 BBa_B0012 component2085984 1 BBa_B0034 component2085989 1 BBa_B0010 component2085978 1 BBa_R0040 component2085988 1 BBa_K343001 annotation2085988 1 BBa_K343001 range2085988 1 81 1943 annotation2085984 1 BBa_B0034 range2085984 1 63 74 annotation2085989 1 BBa_B0010 range2085989 1 1952 2031 annotation2085991 1 BBa_B0012 range2085991 1 2040 2080 annotation2085978 1 BBa_R0040 range2085978 1 1 54 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K343001 1 ninaB B-carotene monooxygenase, Produces retinal from B-carotene. ninaB gene from Drosophila. 2010-07-01T11:00:00Z 2015-05-08T01:12:08Z Sequence taken from cDNA This coding region encodes a beta-carotene deoxygenase, that splits beta-carotene into two retinal molecules. false false _465_ 0 6083 9 It's complicated true No considerations have been made so far concerning design false Christian Kurtzhals annotation2072319 1 Coding sequence range2072319 1 1 1860 annotation2072321 1 stop range2072321 1 1861 1863 annotation2072320 1 met range2072320 1 1 3 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K343001_sequence 1 atggcagccggtgtcttcaagagttttatgcgcgacttctttgcggtgaaatacgatgagcagcgaaatgatccgcaagcggaacgactggatggcaacggacgactgtatcccaactgctcgtcggatgtgtggctgcgatcctgcgagcgggagatagttgatcccattgagggccatcacagcgggcacattcccaaatggatatgcggtagtctgttgcgcaatggacccggcagctggaaggtgggcgacatgaccttcggccatctgttcgactgctccgccctgctgcaccgatttgccattcggaatggacgcgtcacctaccagaatcgcttcgtggacacggaaacactgcgaaagaatcgctctgcccagcggattgtggtcacggagtttggcacagctgctgttccggatccctgtcactcgatcttcgatagatttgcggccatttttcgaccggatagtggaacggataactcgatgatttccatatatcctttcggggatcagtattacacatttacggagacgccttttatgcatagaataaatccctgcactttggccaccgaagcacgaatctgcaccaccgacttcgtgggcgtggtgaaccacacatcgcatccgcatgttcttcccagtggcactgtctacaacctgggcaccacaatgaccagatctggaccggcatacactatactcagtttcccgcacggcgagcagatgttcgaggatgctcatgtggtggccacactgccgtgccgctggaaactgcatcccggttatatgcacaccttcggcttaacggatcactactttgtgattgtggagcagccgttgtccgtttcgcttacggagtatatcaaagcccagctaggtggacagaatttatcggcgtgtctcaagtggttcgaggatcgaccgacactatttcaccttatagatcgggtttccggcaaactggtgcagacctacgaatcggaagccttcttctacctgcacatcatcaactgctttgaacgggatggccacgtggtggtggacatttgcagctacaggaatcccgagatgatcaactgcatgtatctggaggccattgccaatatgcaaacgaatcccaattatgctaccctctttcgtggacgtcccttgagattcgtcctgcccttgggcacaattcctccggcaagcatcgccaagcggggactggtcaagtccttctcccttgctggactaagtgctccgcaggtttctcgcaccatgaagcactcggtctcgcaatatgcggatataacctacatgcccaccaatggaaagcaagccactgctggagaggaaagccccaagcgagatgccaaacgtggccgctatgaggaggagaatcttgtcaatctggttaccatggagggcagtcaagcggaggcgtttcagggcaccaatggcatccaactgcgtccggaaatgctgtgtgattggggctgtgaaacacctaggatctattatgaacggtatatgggcaagaactaccgatacttctacgcgattagctccgatgtggatgcagtgaatccgggcaccctcatcaaggtggatgtgtggaataagagctgtctaacctggtgcgaggagaatgtctatcccagtgagcccatttttgtgccttcgccggatccgaaatccgaggacgatggcgttatcctggcctccatggtgctgggcggtctcaacgatcgctatgtgggcctaattgtgctatgtgccaaaacgatgaccgagctgggccgttgtgatttccataccaatggacccgtgcccaagtgtctccatggatggtttgcacccaatgccatttag BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K343006_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcagccggtgtcttcaagagttttatgcgcgacttctttgcggtgaaatacgatgagcagcgaaatgatccgcaagcggaacgactggatggcaacggacgactgtatcccaactgctcgtcggatgtgtggctgcgatcctgcgagcgggagatagttgatcccattgagggccatcacagcgggcacattcccaaatggatatgcggtagtctgttgcgcaatggacccggcagctggaaggtgggcgacatgaccttcggccatctgttcgactgctccgccctgctgcaccgatttgccattcggaatggacgcgtcacctaccagaatcgcttcgtggacacggaaacactgcgaaagaatcgctctgcccagcggattgtggtcacggagtttggcacagctgctgttccggatccctgtcactcgatcttcgatagatttgcggccatttttcgaccggatagtggaacggataactcgatgatttccatatatcctttcggggatcagtattacacatttacggagacgccttttatgcatagaataaatccctgcactttggccaccgaagcacgaatctgcaccaccgacttcgtgggcgtggtgaaccacacatcgcatccgcatgttcttcccagtggcactgtctacaacctgggcaccacaatgaccagatctggaccggcatacactatactcagtttcccgcacggcgagcagatgttcgaggatgctcatgtggtggccacactgccgtgccgctggaaactgcatcccggttatatgcacaccttcggcttaacggatcactactttgtgattgtggagcagccgttgtccgtttcgcttacggagtatatcaaagcccagctaggtggacagaatttatcggcgtgtctcaagtggttcgaggatcgaccgacactatttcaccttatagatcgggtttccggcaaactggtgcagacctacgaatcggaagccttcttctacctgcacatcatcaactgctttgaacgggatggccacgtggtggtggacatttgcagctacaggaatcccgagatgatcaactgcatgtatctggaggccattgccaatatgcaaacgaatcccaattatgctaccctctttcgtggacgtcccttgagattcgtcctgcccttgggcacaattcctccggcaagcatcgccaagcggggactggtcaagtccttctcccttgctggactaagtgctccgcaggtttctcgcaccatgaagcactcggtctcgcaatatgcggatataacctacatgcccaccaatggaaagcaagccactgctggagaggaaagccccaagcgagatgccaaacgtggccgctatgaggaggagaatcttgtcaatctggttaccatggagggcagtcaagcggaggcgtttcagggcaccaatggcatccaactgcgtccggaaatgctgtgtgattggggctgtgaaacacctaggatctattatgaacggtatatgggcaagaactaccgatacttctacgcgattagctccgatgtggatgcagtgaatccgggcaccctcatcaaggtggatgtgtggaataagagctgtctaacctggtgcgaggagaatgtctatcccagtgagcccatttttgtgccttcgccggatccgaaatccgaggacgatggcgttatcctggcctccatggtgctgggcggtctcaacgatcgctatgtgggcctaattgtgctatgtgccaaaacgatgaccgagctgggccgttgtgatttccataccaatggacccgtgcccaagtgtctccatggatggtttgcacccaatgccatttagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z