BBa_K345667 1 BBa_K345667 Lac regulated insertable promoter 2010-10-23T11:00:00Z 2015-05-08T01:12:08Z The part BBa_J04500 was used as a template for the creation of this new part The part BBa_J04500 was used as a template for the creation of this new part. The -35 and -10 promoter elements were converted through mutagenesis into AflII and ClaI (respectively) restriction sites. A double restriction digest was performed and a T7 promoter was introduced in the form of primers. false false _547_ 0 7437 9 It's complicated true We wanted a T7 promoter that could be regulated with the lac system. false Florencia Camus annotation2114591 1 BBa_K345667 range2114591 1 1 220 annotation2114592 1 CAP binding site range2114592 1 86 127 annotation2114596 1 B0034 range2114596 1 209 220 annotation2114590 1 end of LacI coding region (inactive) range2114590 1 1 85 annotation2114594 1 AflII restriction site range2114594 1 136 141 annotation2114593 1 LacI binding site range2114593 1 164 198 annotation2114595 1 ClaI restriction site range2114595 1 156 161 BBa_K345667_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggcttaagactttatgcttccgcatcgattgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z