LOCUS       BBa_K345669             2072 bp    DNA     linear       25-Nov-2024
DEFINITION  psb1c3 -> BsiWI
ACCESSION   BBa_K345669
VERSION     BBa_K345669.1  
FEATURES             Location/Qualifiers
     misc_feature    1..4
                     /label=LIC site (BsiWI)
     misc_feature    2071..2072
                     /label=LIC site (BsiWI)
     stem_loop       22..114
                     /label=E.coli his operon terminator
     primer_bind     157..176
                     /label=verification reverse(VR) primer binding site
     RBS             261..875
                     /label=rep(pMB1)
     stem_loop       1032..1147
                     /label=T0 terminator
     misc_feature    1262..1831
                     /label=chloramphenicol resistance
     primer_bind     1932..1952
                     /label=verification forward (VF2) primer binding site
     stem_loop       2009..2012
                     /label=end of terminator
     stem_loop       2022..2041
                     /label=stem loop
ORIGIN
        1 tacgagcagc ggccggtgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct
       61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     1081 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     1141 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
     1201 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     1261 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     1321 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     1381 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     1441 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     1501 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     1561 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     1621 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     1681 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     1741 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     1801 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     1861 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
     1921 gatcaactcg agtgccactt gacgtctaag aaaccattat tatcatgaca ttaacctata
     1981 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     2041 atgatttctg gaatttgcgg ccgcttctcg cg
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