BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_I719005 1 pT7 T7 Promoter 2007-10-23T11:00:00Z 2015-08-31T04:07:53Z --- Released HQ 2013 Just a T7 Promoter false false _128_ 0 2097 9 In stock true None true Imperial 2007 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K346034 1 BBa_K346034 T7p(BBa_I719005)+RBS(B0034)+Lpp-OmpA+MBP(surface display of mercury binding peptide) 2010-10-13T11:00:00Z 2015-05-08T01:12:09Z . This part is the combination of a T7promoter(BBa_I719005),RBS(B0034)and Lpp-OmpA+MBP(surface display of mercury binding peptide) false false _457_ 0 6045 9 It's complicated false . false Junyi Jiao annotation2086050 1 BBa_B0032 range2086050 1 32 44 annotation2086049 1 conserved range2086049 1 5 8 annotation2090013 1 lpp-ompa-mbp range2090013 1 51 836 annotation2090012 1 BBa_I719005 range2090012 1 1 23 BBa_K346036 1 BBa_K346036 PT7-RBS-lpp-ompa-mbp-terminator 2010-10-17T11:00:00Z 2015-05-08T01:12:09Z Ptet-T7polymerase+Pb(II) Bioabsorbption Device Ptet-T7polymerase+Pb(II) Bioabsorbption Device false false _457_ 0 5916 9 It's complicated false Ptet-T7polymerase+Pb(II) Bioabsorbption Device false Junyi Jiao component2090038 1 BBa_B0015 component2090031 1 BBa_K346034 annotation2090038 1 BBa_B0015 range2090038 1 845 973 annotation2090031 1 BBa_K346034 range2090031 1 1 836 BBa_K346033 1 BBa_K346033 T7p(BBa_I719005)+RBS(B0034)+DsbA+MBP(periplasm display of mercury binding peptide) 2010-10-13T11:00:00Z 2015-05-08T01:12:09Z . This part is a combination of T7promoter(BBa_I719005),RBS(B0034)and DsbA-MBP(periplasm display of mercury binding peptide) false false _457_ 0 6045 9 It's complicated false . false Liang Donghai Teng Xin annotation2090011 1 BBa_I719005 range2090011 1 1 23 annotation2086047 1 conserved range2086047 1 5 8 annotation2090014 1 Dsba-mbp range2090014 1 51 788 annotation2086048 1 BBa_B0030 range2086048 1 32 44 BBa_K346005 1 BBa_K346005 Mercury (II) ions absorption device 2010-10-13T11:00:00Z 2015-05-08T01:12:09Z to be reassure This part contains three subparts,the dsba-mbp, the mbp and the lpp-ompa-mbp. Each subpart is driven by a T7promoter along with a rbs and terminator, which will express separately in the periplasm,the cytoplasm and the membrane to bind Hg(II). false false _457_ 0 6045 9 It's complicated true to be reassure false Junyi Jiao, Liang Donghai, Hu Yang&Teng Xin component2100582 1 BBa_B0015 component2100595 1 BBa_B0015 component2100575 1 BBa_K346033 component2100608 1 BBa_K346036 component2100588 1 BBa_K346032 annotation2100588 1 BBa_K346032 range2100588 1 934 1306 annotation2100575 1 BBa_K346033 range2100575 1 1 788 annotation2100582 1 BBa_B0015 range2100582 1 797 925 annotation2100595 1 BBa_B0015 range2100595 1 1315 1443 annotation2100608 1 BBa_K346036 range2100608 1 1452 2424 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K346003 1 BBa_K346003 RBS(B0032)+MBP(mercury metal binding peptide engineered from MerR) 2010-10-13T11:00:00Z 2015-07-21T02:51:54Z Tn This part is a combination of a rbs, a mbp, coding a mercury metal binding peptide egineered from MerR, and a terminator. false false _457_ 4206 6045 9 It's complicated true no false Huyang Tengxin annotation2090101 1 conserved range2090101 1 5 8 annotation2090102 1 mbp(mercury binding peptide) range2090102 1 19 342 annotation2090100 1 BBa_B0032 range2090100 1 1 13 BBa_K346032 1 BBa_K346032 T7promoter(I719005)_RBS(B0034)_MBP(mercury metal binding peptide) 2010-10-13T11:00:00Z 2015-05-08T01:12:09Z . This part is a combination of T7promoter(I719005),RBS(B0034) and MBP(mercury metal binding peptide). false false _457_ 0 6045 9 It's complicated false . false yang hu component2100566 1 BBa_I719005 component2100570 1 BBa_K346003 annotation2100570 1 BBa_K346003 range2100570 1 32 373 annotation2100566 1 BBa_I719005 range2100566 1 1 23 BBa_K346036_sequence 1 taatacgactcactatagggagatactagagtcacacaggaaagtactagatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggagtcgacatgacacactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgagcgcttggagccacccgcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K346034_sequence 1 taatacgactcactatagggagatactagagtcacacaggaaagtactagatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggagtcgacatgacacactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgagcgcttggagccacccgcag BBa_I719005_sequence 1 taatacgactcactatagggaga BBa_K346032_sequence 1 taatacgactcactatagggagatactagagaaagaggagaaatactagatgacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagtctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgtaa BBa_K346003_sequence 1 aaagaggagaaatactagatgacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagtctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgtaa BBa_K346033_sequence 1 taatacgactcactatagggagatactagagtcacacaggaaagtactagatgaaaaagatttggctggcgctggctggtttagttttagcgtttagcgcatcggcggcgcagtatgaagatggtaaacagtacactaccctggaaaaaccggtagctggcgcgccgcaagtgctggagtttttctctttcttctgcccgcactgctatcagtttgaagaagttctgcatatttctgataatgtgaagaaaaaactgccggaaggcgtgaagatgactaaataccacgtcaacttcatgggtggtgacctgggcaaagatctgactcaggcatgggctgtggcgatggcgctgggcgtggaagacaaagtgactgttccgctgtttgaaggcgtacagaaaacccagaccattcgttctgcttctgatatccgcgatgtatttatcaacgcaggtattaaaggtgaagagtacgacgcggcgtggaacagcttcgtggtgaaatctctggtcgctcagcaggaaaaagctgcagctgacgtgcaattgcgtggcgttccggcgatgtttgttaacggtaaatatcagctgaatccgcagggtatggataccagcaatatggatgtttttgttcagcagtatgctgatacagtgaaatacttaagcgagaaaaaaggatcaactagaggttctggtacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctg BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K346005_sequence 1 taatacgactcactatagggagatactagagtcacacaggaaagtactagatgaaaaagatttggctggcgctggctggtttagttttagcgtttagcgcatcggcggcgcagtatgaagatggtaaacagtacactaccctggaaaaaccggtagctggcgcgccgcaagtgctggagtttttctctttcttctgcccgcactgctatcagtttgaagaagttctgcatatttctgataatgtgaagaaaaaactgccggaaggcgtgaagatgactaaataccacgtcaacttcatgggtggtgacctgggcaaagatctgactcaggcatgggctgtggcgatggcgctgggcgtggaagacaaagtgactgttccgctgtttgaaggcgtacagaaaacccagaccattcgttctgcttctgatatccgcgatgtatttatcaacgcaggtattaaaggtgaagagtacgacgcggcgtggaacagcttcgtggtgaaatctctggtcgctcagcaggaaaaagctgcagctgacgtgcaattgcgtggcgttccggcgatgtttgttaacggtaaatatcagctgaatccgcagggtatggataccagcaatatggatgtttttgttcagcagtatgctgatacagtgaaatacttaagcgagaaaaaaggatcaactagaggttctggtacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaatacgactcactatagggagatactagagaaagaggagaaatactagatgacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagtctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaatacgactcactatagggagatactagagtcacacaggaaagtactagatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggagtcgacatgacacactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgagcgcttggagccacccgcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z