BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_I719005
1
pT7
T7 Promoter
2007-10-23T11:00:00Z
2015-08-31T04:07:53Z
---
Released HQ 2013
Just a T7 Promoter
false
false
_128_
0
2097
9
In stock
true
None
true
Imperial 2007
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K346034
1
BBa_K346034
T7p(BBa_I719005)+RBS(B0034)+Lpp-OmpA+MBP(surface display of mercury binding peptide)
2010-10-13T11:00:00Z
2015-05-08T01:12:09Z
.
This part is the combination of a T7promoter(BBa_I719005),RBS(B0034)and Lpp-OmpA+MBP(surface display of mercury binding peptide)
false
false
_457_
0
6045
9
It's complicated
false
.
false
Junyi Jiao
annotation2086050
1
BBa_B0032
range2086050
1
32
44
annotation2086049
1
conserved
range2086049
1
5
8
annotation2090013
1
lpp-ompa-mbp
range2090013
1
51
836
annotation2090012
1
BBa_I719005
range2090012
1
1
23
BBa_K346036
1
BBa_K346036
PT7-RBS-lpp-ompa-mbp-terminator
2010-10-17T11:00:00Z
2015-05-08T01:12:09Z
Ptet-T7polymerase+Pb(II) Bioabsorbption Device
Ptet-T7polymerase+Pb(II) Bioabsorbption Device
false
false
_457_
0
5916
9
It's complicated
false
Ptet-T7polymerase+Pb(II) Bioabsorbption Device
false
Junyi Jiao
component2090038
1
BBa_B0015
component2090031
1
BBa_K346034
annotation2090038
1
BBa_B0015
range2090038
1
845
973
annotation2090031
1
BBa_K346034
range2090031
1
1
836
BBa_K346033
1
BBa_K346033
T7p(BBa_I719005)+RBS(B0034)+DsbA+MBP(periplasm display of mercury binding peptide)
2010-10-13T11:00:00Z
2015-05-08T01:12:09Z
.
This part is a combination of T7promoter(BBa_I719005),RBS(B0034)and DsbA-MBP(periplasm display of mercury binding peptide)
false
false
_457_
0
6045
9
It's complicated
false
.
false
Liang Donghai Teng Xin
annotation2090011
1
BBa_I719005
range2090011
1
1
23
annotation2086047
1
conserved
range2086047
1
5
8
annotation2090014
1
Dsba-mbp
range2090014
1
51
788
annotation2086048
1
BBa_B0030
range2086048
1
32
44
BBa_K346005
1
BBa_K346005
Mercury (II) ions absorption device
2010-10-13T11:00:00Z
2015-05-08T01:12:09Z
to be reassure
This part contains three subparts,the dsba-mbp, the mbp and the lpp-ompa-mbp. Each subpart is driven by a T7promoter along with a rbs and terminator, which will express separately in the periplasm,the cytoplasm and the membrane to bind Hg(II).
false
false
_457_
0
6045
9
It's complicated
true
to be reassure
false
Junyi Jiao, Liang Donghai, Hu Yang&Teng Xin
component2100582
1
BBa_B0015
component2100595
1
BBa_B0015
component2100575
1
BBa_K346033
component2100608
1
BBa_K346036
component2100588
1
BBa_K346032
annotation2100588
1
BBa_K346032
range2100588
1
934
1306
annotation2100575
1
BBa_K346033
range2100575
1
1
788
annotation2100582
1
BBa_B0015
range2100582
1
797
925
annotation2100595
1
BBa_B0015
range2100595
1
1315
1443
annotation2100608
1
BBa_K346036
range2100608
1
1452
2424
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K346003
1
BBa_K346003
RBS(B0032)+MBP(mercury metal binding peptide engineered from MerR)
2010-10-13T11:00:00Z
2015-07-21T02:51:54Z
Tn
This part is a combination of a rbs, a mbp, coding a mercury metal binding peptide egineered from MerR, and a terminator.
false
false
_457_
4206
6045
9
It's complicated
true
no
false
Huyang Tengxin
annotation2090101
1
conserved
range2090101
1
5
8
annotation2090102
1
mbp(mercury binding peptide)
range2090102
1
19
342
annotation2090100
1
BBa_B0032
range2090100
1
1
13
BBa_K346032
1
BBa_K346032
T7promoter(I719005)_RBS(B0034)_MBP(mercury metal binding peptide)
2010-10-13T11:00:00Z
2015-05-08T01:12:09Z
.
This part is a combination of T7promoter(I719005),RBS(B0034) and MBP(mercury metal binding peptide).
false
false
_457_
0
6045
9
It's complicated
false
.
false
yang hu
component2100566
1
BBa_I719005
component2100570
1
BBa_K346003
annotation2100570
1
BBa_K346003
range2100570
1
32
373
annotation2100566
1
BBa_I719005
range2100566
1
1
23
BBa_K346036_sequence
1
taatacgactcactatagggagatactagagtcacacaggaaagtactagatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggagtcgacatgacacactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgagcgcttggagccacccgcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K346034_sequence
1
taatacgactcactatagggagatactagagtcacacaggaaagtactagatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggagtcgacatgacacactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgagcgcttggagccacccgcag
BBa_I719005_sequence
1
taatacgactcactatagggaga
BBa_K346032_sequence
1
taatacgactcactatagggagatactagagaaagaggagaaatactagatgacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagtctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgtaa
BBa_K346003_sequence
1
aaagaggagaaatactagatgacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagtctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgtaa
BBa_K346033_sequence
1
taatacgactcactatagggagatactagagtcacacaggaaagtactagatgaaaaagatttggctggcgctggctggtttagttttagcgtttagcgcatcggcggcgcagtatgaagatggtaaacagtacactaccctggaaaaaccggtagctggcgcgccgcaagtgctggagtttttctctttcttctgcccgcactgctatcagtttgaagaagttctgcatatttctgataatgtgaagaaaaaactgccggaaggcgtgaagatgactaaataccacgtcaacttcatgggtggtgacctgggcaaagatctgactcaggcatgggctgtggcgatggcgctgggcgtggaagacaaagtgactgttccgctgtttgaaggcgtacagaaaacccagaccattcgttctgcttctgatatccgcgatgtatttatcaacgcaggtattaaaggtgaagagtacgacgcggcgtggaacagcttcgtggtgaaatctctggtcgctcagcaggaaaaagctgcagctgacgtgcaattgcgtggcgttccggcgatgtttgttaacggtaaatatcagctgaatccgcagggtatggataccagcaatatggatgtttttgttcagcagtatgctgatacagtgaaatacttaagcgagaaaaaaggatcaactagaggttctggtacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctg
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K346005_sequence
1
taatacgactcactatagggagatactagagtcacacaggaaagtactagatgaaaaagatttggctggcgctggctggtttagttttagcgtttagcgcatcggcggcgcagtatgaagatggtaaacagtacactaccctggaaaaaccggtagctggcgcgccgcaagtgctggagtttttctctttcttctgcccgcactgctatcagtttgaagaagttctgcatatttctgataatgtgaagaaaaaactgccggaaggcgtgaagatgactaaataccacgtcaacttcatgggtggtgacctgggcaaagatctgactcaggcatgggctgtggcgatggcgctgggcgtggaagacaaagtgactgttccgctgtttgaaggcgtacagaaaacccagaccattcgttctgcttctgatatccgcgatgtatttatcaacgcaggtattaaaggtgaagagtacgacgcggcgtggaacagcttcgtggtgaaatctctggtcgctcagcaggaaaaagctgcagctgacgtgcaattgcgtggcgttccggcgatgtttgttaacggtaaatatcagctgaatccgcagggtatggataccagcaatatggatgtttttgttcagcagtatgctgatacagtgaaatacttaagcgagaaaaaaggatcaactagaggttctggtacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaatacgactcactatagggagatactagagaaagaggagaaatactagatgacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagtctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaatacgactcactatagggagatactagagtcacacaggaaagtactagatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggagtcgacatgacacactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgagcgcttggagccacccgcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z