BBa_K346018 1 BBa_K346018 PmerT promoter(88)+RBS(B0034)+lacZ alpha(BBa_I732006) 2010-10-17T11:00:00Z 2015-05-08T01:12:09Z PmerT promoter(88)+RBS(B0034)+lacZ alpha(BBa_I732006) PmerT promoter(88)+RBS(B0034)+lacZ alpha(BBa_I732006) false false _457_ 0 5916 9 It's complicated false PmerT promoter(88)+RBS(B0034)+lacZ alpha(BBa_I732006) false Zairan Liu component2094877 1 BBa_B0034 component2094881 1 BBa_I732006 component2094875 1 BBa_K346060 annotation2094877 1 BBa_B0034 range2094877 1 66 77 annotation2094875 1 BBa_K346060 range2094875 1 1 57 annotation2094881 1 BBa_I732006 range2094881 1 84 317 BBa_I732006 1 lacZ-alpha lacZ alpha fragment 2007-07-06T11:00:00Z 2015-08-31T04:07:56Z PCR amplified from BBa_J33202 without the RBS. Released HQ 2013 In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity. false true _156_ 0 1557 9 In stock false None true Zhan Jian annotation1936904 1 lacZ range1936904 1 1 234 annotation1936906 1 STOP range1936906 1 229 234 annotation1936905 1 START range1936905 1 1 3 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K346060 1 BBa_K346060 PmerT promoter mutant 88 2010-10-17T11:00:00Z 2015-05-08T01:12:09Z 88 mutation of PmerT 88 mutation of PmerT false false _457_ 0 5916 9 It's complicated false 88 mutation of PmerT false Ao Liu BBa_B0034_sequence 1 aaagaggagaaa BBa_K346060_sequence 1 ttccatatcgcttgacgccgtacatgagtacggaagtaaggttacgctatccaatcc BBa_K346018_sequence 1 ttccatatcgcttgacgccgtacatgagtacggaagtaaggttacgctatccaatcctactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa BBa_I732006_sequence 1 atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z