LOCUS BBa_K346035 5167 bp DNA linear 22-Dec-2024 DEFINITION Ptet-T7polymerase+Hg(II) Bioabsorption Device ACCESSION BBa_K346035 VERSION BBa_K346035.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 32..44 /label=BBa_B0030 promoter 1..23 /label=BBa_I719005 CDS 51..788 /label=Dsba-mbp misc_feature 1..788 /label=BBa_K346033 stem_loop 808..851 /label=stem_loop misc_feature 797..876 /label=BBa_B0010 terminator 797..876 /label=BBa_B0010 stem_loop 892..911 /label=T7 TE misc_feature 918..918 /label=stop polyA_site 912..925 /label=polya misc_feature 885..925 /label=BBa_B0012 terminator 885..925 /label=BBa_B0012 terminator 797..925 /label=BBa_B0015 promoter 934..956 /label=BBa_I719005 misc_feature 965..977 /label=BBa_B0032 misc_feature 969..972 /label=conserved CDS 983..1306 /label=mbp(mercury binding peptide) misc_feature 965..1306 /label=BBa_K346003 misc_feature 934..1306 /label=BBa_K346032 stem_loop 1326..1369 /label=stem_loop misc_feature 1315..1394 /label=BBa_B0010 terminator 1315..1394 /label=BBa_B0010 stem_loop 1410..1429 /label=T7 TE misc_feature 1436..1436 /label=stop polyA_site 1430..1443 /label=polya misc_feature 1403..1443 /label=BBa_B0012 terminator 1403..1443 /label=BBa_B0012 terminator 1315..1443 /label=BBa_B0015 misc_feature 1456..1459 /label=conserved RBS 1483..1495 /label=BBa_B0032 promoter 1452..1474 /label=BBa_I719005 CDS 1502..2287 /label=lpp-ompa-mbp misc_feature 1452..2287 /label=BBa_K346034 stem_loop 2307..2350 /label=stem_loop misc_feature 2296..2375 /label=BBa_B0010 terminator 2296..2375 /label=BBa_B0010 stem_loop 2391..2410 /label=T7 TE misc_feature 2417..2417 /label=stop polyA_site 2411..2424 /label=polya misc_feature 2384..2424 /label=BBa_B0012 terminator 2384..2424 /label=BBa_B0012 terminator 2296..2424 /label=BBa_B0015 misc_feature 1452..2424 /label=BBa_K346036 misc_feature 1..2424 /label=BBa_K346005 RBS 2507..2512 /label=rbs misc_feature 2437..2440 /label=conserved CDS 2513..5167 /label=T7polymerase promoter 2433..2506 /label=Ptet misc_feature 2433..5167 /label=BBa_K346085 ORIGIN 1 taatacgact cactataggg agatactaga gtcacacagg aaagtactag atgaaaaaga 61 tttggctggc gctggctggt ttagttttag cgtttagcgc atcggcggcg cagtatgaag 121 atggtaaaca gtacactacc ctggaaaaac cggtagctgg cgcgccgcaa gtgctggagt 181 ttttctcttt cttctgcccg cactgctatc agtttgaaga agttctgcat atttctgata 241 atgtgaagaa aaaactgccg gaaggcgtga agatgactaa ataccacgtc aacttcatgg 301 gtggtgacct gggcaaagat ctgactcagg catgggctgt ggcgatggcg ctgggcgtgg 361 aagacaaagt gactgttccg ctgtttgaag gcgtacagaa aacccagacc attcgttctg 421 cttctgatat ccgcgatgta tttatcaacg caggtattaa aggtgaagag tacgacgcgg 481 cgtggaacag cttcgtggtg aaatctctgg tcgctcagca ggaaaaagct gcagctgacg 541 tgcaattgcg tggcgttccg gcgatgtttg ttaacggtaa atatcagctg aatccgcagg 601 gtatggatac cagcaatatg gatgtttttg ttcagcagta tgctgataca gtgaaatact 661 taagcgagaa aaaaggatca actagaggtt ctggtaccca ctgcgaggag gccagcagcc 721 tggccgaaca caagctcaag gacgtgcgcg agaagatggc cgacttggcg cgcatggaaa 781 ccgtgctgta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 841 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 901 tcgggtgggc ctttctgcgt ttatatacta gagtaatacg actcactata gggagatact 961 agagaaagag gagaaatact agatgaccca ctgcgaggag gccagcagcc tggccgaaca 1021 caagctcaag gacgtgcgcg agaagatggc cgacttggcg cgcatggaaa ccgtgctgtc 1081 tgaactcgtg tgcgcctgcc atgcacgaaa ggggaatgtt tcctgcccgt tgatcgcgtc 1141 actacaggga tcctcaggca cccactgcga ggaggccagc agtctggccg aacacaagct 1201 caaggacgtg cgcgagaaga tggccgactt ggcgcgcatg gaaaccgtgc tgtctgaact 1261 cgtgtgcgcc tgccatgcac gaaaggggaa tgtttcctgc ccgtaatact agagccaggc 1321 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 1381 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 1441 atatactaga gtaatacgac tcactatagg gagatactag agtcacacag gaaagtacta 1501 gatgaaagct actaaactgg tactgggcgc ggtaatcctg ggttctactc tgctggcagg 1561 ttgctccagc aacgctaaaa tcgatcaggg aattaacccg tatgttggct ttgaaatggg 1621 ttacgactgg ttaggtcgta tgccgtacaa aggcagcgtt gaaaacggtg catacaaagc 1681 tcagggcgtt caactgaccg ctaaactggg ttacccaatc actgacgacc tggacatcta 1741 cactcgtctg ggtggcatgg tatggcgtgc agacactaaa tccaacgttt atggtaaaaa 1801 ccacgacacc ggcgtttctc cggtcttcgc tggcggtgtt gagtacgcga tcactcctga 1861 aatcgctacc cgtctggaat accagtggac caacaacatc ggtgacgcac acaccatcgg 1921 cactcgtccg gacaacggag tcgacatgac acactgcgag gaggccagca gcctggccga 1981 acacaagctc aaggacgtgc gcgagaagat ggccgacttg gcgcgcatgg aaaccgtgct 2041 gtctgaactc gtgtgcgcct gccatgcacg aaaggggaat gtttcctgcc cgttgatcgc 2101 gtcactacag ggatcctcag gcacccactg cgaggaggcc agcagcctgg ccgaacacaa 2161 gctcaaggac gtgcgcgaga agatggccga cttggcgcgc atggaaaccg tgctgtctga 2221 actcgtgtgc gcctgccatg cacgaaaggg gaatgtttcc tgcccgagcg cttggagcca 2281 cccgcagtac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 2341 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 2401 cgggtgggcc tttctgcgtt tatatactag agtccctatc agtgatagag attgacatcc 2461 ctatcagtga tagagatact gagcactact agagaaagag gagaaatact agatgaacac 2521 gattaacatc gctaagaacg acttctctga catcgaactg gctgctatcc cgttcaacac 2581 tctggctgac cattacggtg agcgtttagc tcgcgaacag ttggcccttg agcatgagtc 2641 ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta aagctggtga 2701 ggttgcggat aacgctgccg ccaagcctct catcactacc ctactcccta agatgattgc 2761 acgcatcaac gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt 2821 ccagttcctg caagaaatca agccggaagc cgtagcgtac atcaccatta agaccactct 2881 ggcttgccta accagtgctg acaatacaac cgttcaggct gtagcaagcg caatcggtcg 2941 ggccattgag gacgaggctc gcttcggtcg tatccgtgac cttgaagcta agcacttcaa 3001 gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac gtctacaaga aagcatttat 3061 gcaagttgtc gaggctgaca tgctctctaa gggtctactc ggtggcgagg cgtggtcttc 3121 gtggcataag gaagactcta ttcatgtagg agtacgctgc atcgagatgc tcattgagtc 3181 aaccggaatg gttagcttac accgccaaaa tgctggcgta gtaggtcaag actctgagac 3241 tatcgaactc gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg 3301 catctctccg atgttccaac cttgcgtagt tcctcctaag ccgtggactg gcattactgg 3361 tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa 3421 agcactgatg cgctacgaag acgtttacat gcctgaggtg tacaaagcga ttaacattgc 3481 gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa 3541 gtggaagcat tgtccggtcg aggacatccc tgcgattgag cgtgaagaac tcccgatgaa 3601 accggaagac atcgacatga atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc 3661 tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga 3721 gcaagccaat aagtttgcta accataaggc catctggttc ccttacaaca tggactggcg 3781 cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt aacgatatga ccaaaggact 3841 gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt tactactggc tgaaaatcca 3901 cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct gagcgcatca agttcattga 3961 ggaaaaccac gagaacatca tggcttgcgc taagtctcca ctggagaaca cttggtgggc 4021 tgagcaagat tctccgttct gcttccttgc gttctgcttt gagtacgctg gggtacagca 4081 ccacggcctg agctataact gctcccttcc gctggcgttt gacgggtctt gctctggcat 4141 ccagcacttc tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta acttgcttcc 4201 tagtgaaacc gttcaggaca tctacgggat tgttgctaag aaagtcaacg agattctaca 4261 agcagacgca atcaatggga ccgataacga agtagttacc gtgaccgatg agaacactgg 4321 tgaaatctct gagaaagtca agctgggcac taaggcactg gctggtcaat ggctggctta 4381 cggtgttact cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga 4441 gttcggcttc cgtcaacaag tgctggaaga taccattcag ccagctattg attccggcaa 4501 gggtctgatg ttcactcagc cgaatcaggc tgctggatac atggctaagc tgatttggga 4561 atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg aactggctta agtctgctgc 4621 taagctgctg gctgctgagg tcaaagataa gaagactgga gagattcttc gcaagcgttg 4681 cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg caggaataca agaagcctat 4741 tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc ttacagccta ccattaacac 4801 caacaaagat agcgagattg atgcacacaa acaggagtct ggtatcgctc ctaactttgt 4861 acacagccaa gacggtagcc accttcgtaa gactgtagtg tgggcacacg agaagtacgg 4921 aatcgaatct tttgcactga ttcacgactc cttcggtacc attccggctg acgctgcgaa 4981 cctgttcaaa gcagtgcgcg aaactatggt tgacacatat gagtcttgtg atgtactggc 5041 tgatttctac gaccagttcg ctgaccagtt gcacgagtct caattggaca aaatgccagc 5101 acttccggct aaaggtaact tgaacctccg tgacatctta gagtcggact tcgcgttcgc 5161 gtaataa //