LOCUS       BBa_K346053             2875 bp    DNA     linear       23-Dec-2024
DEFINITION  PpbrA promoter+T3 polymerase+Terminator(B0015)
ACCESSION   BBa_K346053
VERSION     BBa_K346053.1  
FEATURES             Location/Qualifiers
     promoter        1..56
                     /label=BBa_K346054
     CDS             83..2738
                     /label=t3 pol
     RBS             65..78
                     /label=b0032
     misc_feature    65..2738
                     /label=BBa_K346000
     stem_loop       2758..2801
                     /label=stem_loop
     misc_feature    2747..2826
                     /label=BBa_B0010
     terminator      2747..2826
                     /label=BBa_B0010
     stem_loop       2842..2861
                     /label=T7 TE
     misc_feature    2868..2868
                     /label=stop
     polyA_site      2862..2875
                     /label=polya
     misc_feature    2835..2875
                     /label=BBa_B0012
     terminator      2835..2875
                     /label=BBa_B0012
     terminator      2747..2875
                     /label=BBa_B0015
ORIGIN
        1 tgggagatgt cttgactcta tagtaactag agggtgttaa atcggcaacg cgagaatact
       61 agagtcacac aggaaagtac tagatgaaca tcatcgaaaa catcgaaaag aatgacttct
      121 cagaaatcga actggctgct atcccgttca acacactggc tgaccactac ggaagcgcct
      181 tggctaaaga gcagttggct ttagaacatg agtcttatga gctaggcgag cgccgcttcc
      241 tcaagatgct tgagcgtcaa gcgaaagctg gtgagattgc agacaacgca gccgctaagc
      301 cgttactcgc tacgcttctc cctaagttaa ccacacgtat cgtcgagtgg ctcgaagagt
      361 acgcatcgaa gaaaggccgc aagcctagcg catacgcacc gctccagtta ctcaagccgg
      421 aggcctccgc gtttatcacc ctgaaagtta tccttgcgtc actaaccagt acgaacatga
      481 caaccattca ggccgctgct ggtatgctgg ggaaagccat tgaggacgag gcacgatttg
      541 ggcgcatccg tgacctagaa gcgaagcact tcaagaagca cgttgaggaa cagcttaaca
      601 agcgccacgg gcaagtctac aagaaagcat ttatgcaggt ggtcgaggcc gatatgattg
      661 gtcgaggtct gcttggtggc gaggcgtggt ctagctggga taaagaaacc acgatgcacg
      721 tagggattcg cctgattgaa atgctgattg aatccacggg tctggtggaa ttacagcgcc
      781 acaacgcagg taacgcaggc tctgaccatg aggcactgca actggcccaa gagtacgtgg
      841 acgtattagc gaagcgtgca ggcgctctgg cgggtatctc tccgatgttc cagccgtgtg
      901 tcgtaccgcc gaaaccttgg gtagcaatca cagggggcgg ctattgggct aacggtcgca
      961 gacctttggc actcgttcgc actcactcta agaagggctt gatgcgctac gaagacgttt
     1021 acatgccaga agtctacaag gctgtgaacc tcgcgcaaaa caccgcatgg aaaatcaaca
     1081 agaaagttct tgctgttgtc aatgagattg ttaactggaa gaattgcccg gtagcagaca
     1141 ttccatcgct ggagcgccaa gagttaccgc ctaagcctga cgacattgac accaacgagg
     1201 cagcgctcaa ggagtggaag aaagccgctg ctggtatcta tcgcttggac aaggcacgag
     1261 tgtctcgccg tatcagctta gagttcatgc tggagcaggc caacaagttc gcaagtaaga
     1321 aagcaatctg gttcccttac aacatggact ggcgcggtcg tgtgtacgct gtgccgatgt
     1381 tcaacccgca aggcaacgac atgacgaaag gtctgctgac ccttgctaaa ggcaagccaa
     1441 tcggtgagga aggtttctac tggctgaaaa tccacggtgc gaactgtgcg ggtgttgata
     1501 aggttccatt cccggagcgc atcgcgttca ttgagaagca cgtagacgac attctggctt
     1561 gcgctaaaga cccaatcaat aacacttggt gggctgagca ggattcaccg ttctgtttcc
     1621 tcgcgttttg cttcgagtat gcaggcgtta cgcaccacgg tctgagctac aattgctctc
     1681 tgccgctggc gttcgacggg tcttgctctg gtatccagca cttctccgcg atgctccgcg
     1741 atgaggtagg cggtcgtgcg gttaacctgc tgccaagcga aaccgtgcag gacatttacg
     1801 gcatcgttgc acagaaagta aacgagattc tcaaacagga tgcaatcaac ggcacgccta
     1861 acgagatgat taccgtgacc gacaaggaca ccggggaaat ctcagagaag ctcaaacttg
     1921 gaacctcaac gctggcgcaa cagtggctgg catatggtgt aacccgtagc gtaactaaac
     1981 gttcggtcat gacgctggct tacggttcca aggagttcgg ctttcgtcaa caggtattgg
     2041 atgacaccat tcagcctgca attgacagcg gtaagggctt gatgttcacc caaccgaacc
     2101 aagcggctgg ctatatggct aagctgattt gggatgcggt aagcgtgacc gtagttgcag
     2161 cggttgaggc gatgaactgg ctcaaatctg ccgctaagct gctggctgct gaggtcaagg
     2221 acaagaagac caaggagatt ctgcgccacc gttgcgcggt tcactggact acgccggacg
     2281 gcttcccggt ctggcaggaa taccgcaagc cactccagaa gcgtctcgat atgattttct
     2341 tagggcaatt ccgtctgcaa ccgacgatta ataccctcaa ggattcaggc attgacgcac
     2401 acaagcagga gtctggcatc gctcctaact ttgttcactc acaggacggt agccacctcc
     2461 gcatgacagt cgtttatgct cacgagaagt atggcattga gtcctttgcg ctcatccatg
     2521 acagctttgg gactatcccg gcagacgctg gtaagctctt taaggctgtg cgtgaaacga
     2581 tggttatcac ctatgagaac aacgatgtgc tggcagactt ctactctcag tttgccgacc
     2641 agctacacga gacccaactg gacaagatgc ctccgcttcc gaagaaagga aacctgaacc
     2701 tgcaagacat tctcaagtct gactttgcct ttgcataata ctagagccag gcatcaaata
     2761 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2821 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//