LOCUS       BBa_K346055              942 bp    DNA     linear       22-Dec-2024
DEFINITION  PpbrA promoter-GFP(E0840)
ACCESSION   BBa_K346055
VERSION     BBa_K346055.1  
FEATURES             Location/Qualifiers
     promoter        1..56
                     /label=BBa_K346054
     RBS             65..79
                     /label=RBS-1\Strong
     RBS             72..76
                     /label=RBS
     misc_feature    65..79
                     /label=BBa_B0030
     RBS             65..79
                     /label=BBa_B0030
     CDS             86..805
                     /label=GFP protein
     CDS             86..805
                     /label=BBa_E0040
     stem_loop       825..868
                     /label=stem_loop
     misc_feature    814..893
                     /label=BBa_B0010
     terminator      814..893
                     /label=BBa_B0010
     stem_loop       909..928
                     /label=T7 TE
     misc_feature    935..935
                     /label=stop
     polyA_site      929..942
                     /label=polya
     misc_feature    902..942
                     /label=BBa_B0012
     terminator      902..942
                     /label=BBa_B0012
     misc_feature    65..942
                     /label=BBa_E0840
ORIGIN
        1 tgggagatgt cttgactcta tagtaactag agggtgttaa atcggcaacg cgagaatact
       61 agagattaaa gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt
      121 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      181 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
      241 actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc
      301 gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta
      361 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
      421 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
      481 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
      541 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga
      601 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
      661 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
      721 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
      781 catggatgaa ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc
      841 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
      901 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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