LOCUS       BBa_K346058              532 bp    DNA     linear       22-Nov-2024
DEFINITION  T7promoter(BBa_I719005)+RBS(B0034)+phiR73 delta(BBa_I746352)+Termin
            ator(B0015)+PO promoter(BBa_I7463
ACCESSION   BBa_K346058
VERSION     BBa_K346058.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    32..43
                     /label=B0034
     CDS             53..295
                     /label=phiR73 delta
     misc_feature    53..53
                     /label=phiR73 delta
     misc_feature    133..133
                     /label=silent mutation to remove PstI site
     misc_feature    32..295
                     /label=BBa_I746352
     stem_loop       315..358
                     /label=stem_loop
     misc_feature    304..383
                     /label=BBa_B0010
     terminator      304..383
                     /label=BBa_B0010
     stem_loop       399..418
                     /label=T7 TE
     misc_feature    425..425
                     /label=stop
     polyA_site      419..432
                     /label=polya
     misc_feature    392..432
                     /label=BBa_B0012
     terminator      392..432
                     /label=BBa_B0012
     terminator      304..432
                     /label=BBa_B0015
     promoter        441..532
                     /label=PO
     promoter        441..532
                     /label=BBa_I746361
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgatgcgctg
       61 ccctttctgt cgtcattcag cgcatacccg caccagccgg tatgtgagtg acaatgtcaa
      121 agaaagttat ctccagtgcc agaatattta ctgttcggcg acatttaaaa cgcatgagtc
      181 aatttgtgcc gtgattcgtt ctccggtcac ggaggaaaaa ccagcaccgg caagcacagc
      241 accggctgtt gtccgaaaag ttaaaggctg ttacagctca ccattcaacc attaatacta
      301 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      361 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      421 tctgcgttta tatactagag cgcgccccgc gattcgctaa ggtgctgttg tgtcagtgat
      481 aagccatccg ggactgatgg cggaggatgc gcatcgtcgg gaaactgatg cc
//